Endogenous carbon monoxide (CO) as a modulating factor of molecular biological clock in the hypothalamic structures involved in light transmission in pig and wild boar hybrid during long and short day season

• Autorzy: Gilun P. , Koziorowska-Gilun M. , Wasowska B. , Sowa-Kucma M. , Koziol K. , Romerowicz-Misielak M. , Kordan W. , Koziorowski M.
• Języki publikacji: EN

Abstrakty

EN

Mature males of a wild boar-pig crossbreed, during the long and short day season, were used for the study which demonstrates that the chemical light carrier CO regulates the expression of biological clock genes in the hypothalamus via humoral pathways. Autologous blood with experimentally elevated concentrations of endogenous CO (using lamps with white light-emitting diodes) was infused into the ophthalmic venous sinus via the right dorsal nasal vein. Molecular biology methods: qPCR and Western Blot were used to determine the expression of genes and biological clock proteins. The results showed that elevated endogenous CO levels, through blood irradiation, induces changes in genes expression involved in the functioning of the main biological clock located in suprachiasmatic nuclei. Changes in the expression of the transcription factors Bmal1, Clock and Npas2 have a similar pattern in both structures, where a very large decrease in gene expression was shown after exposure to elevated endogenous CO levels. The changes in the gene expression of PER 1-2, CRY 1-2, and REV-ERB α-β and ROR β are not the same for both POA and DH hypothalamic structures, indicating that both structures respond differently to the humoral signal received. The results indicate that CO is a chemical light molecule whose production in an organism depends on the amount of light. An adequate amount of light is an essential factor for the proper functioning of the main biological clock.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2023
• Tom: 26
• Numer: 1
• Opis fizyczny: p.65-82,fig.,ref.

Twórcy

autor

Gilun P.

• Department of Local Physiological Regulations, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Tuwima 10, 10-748, Olsztyn, Poland

autor

Koziorowska-Gilun M.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, Poland

autor

Wasowska B.

• Department of Local Physiological Regulations, Institute of Animal Reproduction and Food Research of the Polish Academy of Sciences in Olsztyn, Tuwima 10, 10-748, Olsztyn, Poland

autor

Sowa-Kucma M.

• Department of Human Physiology, Institute of Medical Sciences, Medical College of Rzeszow University, Kopisto 2a, Rzeszow, 35-959, Poland

autor

Koziol K.

• Department of Animal Physiology and Reproduction, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland

autor

Romerowicz-Misielak M.

• Department of Animal Physiology and Reproduction, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland

autor

Kordan W.

• Department of Animal Biochemistry and Biotechnology, Faculty of Animal Bioengineering, University of Warmia and Mazury, Oczapowskiego 5, 10-719 Olsztyn, Poland

autor

Koziorowski M.

• Department of Animal Physiology and Reproduction, Institute of Biology and Biotechnology, Collegium Scientarium Naturalium, University of Rzeszow, Werynia 502, 36-100 Kolbuszowa, Poland

Identyfikatory

DOI

10.24425/pjvs.2023.145008