Orientation tuning in mouse primary visual cortex – Intrinsic signal optical imaging study

• Autorzy: Raciborska I. , Kordecka K. , Posluszny A. , Waleszczyk W.J.
• Języki publikacji: EN

Abstrakty

EN

INTRODUCTION: Accumulating body of research has shown a cardinal bias for preference of spatially oriented targets in different species including humans, indicating greater neuronal responses in the primary visual cortex for horizontal or vertical contours in opposite to oblique ones. AIM(S): We used intrinsic signal optical imaging, a popular tool to map cortical function in rodents to verify the hypothesis whether a cardinal bias is present also in mouse primary visual cortex. METHOD(S): The experiments were performed on 7 week old wild mice under isoflurane anaesthesia. Intrinsic signals were recorded using CCD camera set above the visual cortex. Visual stimuli, square-wave black-and-white gratings (spatial frequency 0.05 cycle/degree, and temporal frequency 2 Hz, four orientations: 0, 45, 90, 135 degree) drifting in two directions, back and forth, were presented in random order with uniform grey images in 16 trials. Imaging was performed under the control of Imager 3001 system. Data were collected with 10 Hz resolution from 1 s before stimulus onset, during 7 s of visual stimulation and to 1 s after stimulus offset with 7 s interval between recordings. RESULTS: Using the described protocol of visual stimulation and data collection we could successfully map cortical responses to visual stimuli of different orientations. Collected images showed the strongest responses for horizontally and vertically oriented gratings. CONCLUSIONS: Our results support the hypothesis of the bias toward cardinal orientation preference in mouse visual cortex. FINANCIAL SUPPORT: Supported by the Polish National Science Center grant Symfonia 1 (2013/08/W/ NZ4/00691).

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2017
• Tom: 77
• Numer: Suppl.1
• Opis fizyczny: p.82

Twórcy

autor

Raciborska I.

• Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Kordecka K.

• Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Posluszny A.

• Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland

autor

Waleszczyk W.J.

• Nencki Institute of Experimental Biology Polish Academy of Sciences, Warsaw, Poland