The relationship between extracellular K-plus and Ca2-plus on aminopyrine accumulation in rat parietal cells

• Autorzy: Ostrowski J. , Zych W. , Dolowy K. , Butruk E.
• Języki publikacji: EN

Abstrakty

EN

The effects of extracellular K⁺ in relation to extracellular Ca²⁺ on acid production were studied. Studies were performed in vitro using isolated cells from rat stomachs, and acid production was indirectly determined by ¹⁴C-aminopyrine (AP) accumulation. In the absence of K⁺ in the incubation medium histamine-stimulated AP accumulation ratios were significantly decreased independently in the presence or absence of extracellular Ca²⁺. Under basal conditions, in the absence of extracellular Ca²⁺ , increasing concentrations of extracellular K⁺ enhanced AP accumulation ratios to significantly higher than those found in the presence of Ca²⁺. In histamine-, cAMP-, and carbachol-stimulated parietal cells, high K⁺ concentrations increased AP accumulation significantly less in Ca²⁺-free than in Ca²⁺-containing media. High K⁺ also induced significantly both an increase in cytosolic free Ca²⁺ concentration and ⁴⁵ Ca²⁺ uptake. The present results confirmed the importance of K⁺ in gastric acid production and suggested a role for Ca²⁺ as a modulator of mechanisms of parietal cell stimulation.

Słowa kluczowe

EN

parietal cell; rat; stomach; acid secretion; extracellular potassium; extracellular calcium; aminopyrine accumulation

• Wydawca: -
• Czasopismo: Journal of Physiology and Pharmacology
• Rocznik: 1991
• Tom: 42
• Numer: 3
• Opis fizyczny: p.279-291,fig.,ref.

Twórcy

autor

Ostrowski J.

• Department of Gastroenterology and Metabolism, Medical Center of Postgraduate Education, Goszczynskiego 1, 02-616 Warsaw, Poland

autor

Zych W.

• Department of Gastroenterology and Metabolism, Medical Center of Postgraduate Education, Goszczynskiego 1, 02-616 Warsaw, Poland

autor

Dolowy K.

• Department of Biophysics and Biomathematic, Medical Center of Postgraduate Education, Goszczynskiego 1, 02-616 Warsaw, Poland

autor

Butruk E.

• Department of Gastroenterology and Metabolism, Medical Center of Postgraduate Education, Goszczynskiego 1, 02-616 Warsaw, Poland

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