Cadmium-induced responses in duckweed Lemna minor L.

• Autorzy: Tkalec M. , Prebeg T. , Roje V. , Pevalek-Kozlina B. , Ljubesic N.
• Języki publikacji: EN

Abstrakty

EN

Although duckweed Lemna minor L. is a known accumulator of cadmium, detailed studies on its physiological and/or defense responses to this metal are still lacking. In this study, the effects of 10 μM CdCl2 on Lemna minor were monitored after 6 and 12 days of treatment, while growth was estimated every 2 days. Cadmium treatment resulted in progressive accumulation of the metal in the plants and led to a decrease in the growth rate to 54% of the control value. The metal also considerably impaired chloroplast ultrastructure and caused a significant reduction in pigment content, i.e., at day 12, by 30 and 34% for chlorophylls a and b, and by 25% for carotenoids. During cadmium treatment, the contents of malondialdehyde and endogenous H2O2 progressively increased (rising 77 and 46% above the controls by day 12), indicating that cadmium induced considerable oxidative stress. On the other hand, higher activities of pyrogallol peroxidase (PPX), ascorbate peroxidase (APX) and catalase (CAT), as well as the induction of a new APX isoform, in cadmium-treated plants, clearly showed activation of an antioxidative response. At day 6, only PPX activity was significantly above the controls (15%), while, at day 12, PPX, APX and CAT activities were increased (74, 78 and 63%). Cadmium also led to accumulation of the heat shock protein 70 (HSP70) and induced an additional isoform of this protein. The obtained results suggest that cadmium (10 μM) is phytotoxic to Lemna minor, inducing oxidative stress, and that antioxidative enzymes and HSP70 play important roles in the defense against cadmium toxicity.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2008
• Tom: 30
• Numer: 6
• Opis fizyczny: p.881-890,fig.,ref.

Twórcy

autor

Tkalec M.

• Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Rooseveltov tgr. 6, 10000 Zagreb, Croatia

autor

Prebeg T.

• Division of Molecular Biology, Ruder Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia

autor

Roje V.

• Division for Marine and Environmental Research, Ruder Boscovic Institute, Bijenicka 54, 10000 Zagreb, Croatia

autor

Pevalek-Kozlina B.

• Department of Botany, Division of Biology, Faculty of Science, University of Zagreb, Rooseveltov tgr. 6, 10000 Zagreb, Croatia

autor

Ljubesic N.

• Division of Molecular Biology, Ruder Boskovic Institute, Bijenicka 54, 10000 Zagreb, Croatia

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