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2015 | 14 | 3 |
Tytuł artykułu

Physicochemical and antimicrobial properties of copaiba oil: implications on product quality control

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Background. The copaiba oil is a common natural product used in cosmetic industry and as a nutraceutical product. However, lack of quality control and scarce knowledge about its antimicrobial activity is a point of concern. The proposal of this study was to investigate the physicochemical properties and the antimicrobial activity of five commercial brands of copaiba oil. Material and methods. Acidity and ester index, refractory index, solubility in alcohol, and thin layer chromatography were performed to verify the physicochemical properties of five commercial copaiba oils sold in local pharmacies. Ultra performance liquid chromatography coupled with diode-array detection and electrospray ionization quadrupole time-of-flight mass spectrometry (UPLC-DAD/ESI-Q-TOF-MS) was used to investigate diterpene acids while the volatile compounds were analysed by gas chromatography-mass spectrometry (GC-MS). Antibacterial and antifungal activities were also evaluated by agar diffusion technique; and minimal inhibitory concentration and maximal bactericidal concentration were defined for each sample and bacteria. Results. The physical-chemical analysis revealed heterogeneity between all samples analysed. The A1 sample showed characteristics of copaiba oil and was mainly composed by hydrocarbon sesquiterpenes (29.95% β-bisabolene, 25.65% Z-a-bergamotene and 10.27% β-cariophyllene). Among diterpene acids, the UPLC- DAD/ESI-Q-TOF-MS data are compatible with presence of copalic and/or kolavenic acid (m/z 305 [M + H]+). Candida albicans was sensitive to almost all samples at high concentration and Saccaromyces. cere- visiae showed sensitivity to A1 sample at 100 mg/mL. Although variable, all samples showed antibacterial activity. Significant activity was seen for A3 (19.0 ±0 and 15.6 ±0.5 mm), A4 (16.6 ±0.5 and 15.6 ±0 mm), and A5 (17.1 ±0 and 17.1 ±0 mm) on Staphylococcus saprophyticus and S. aureus, respectively. All samples were active against Klebsiella pneumoniae showing >15 mm diameter halo inhibition; and only A2 was active against Eschirichia coli. Phytopatogens tested revealed resistance of Ralstonia solanacearum CGH12 to all samples and susceptibility of Xcv 112 strain of Xanthomonas campestris pv campestris to almost all samples. MIC and MMC showed bacteriostatic effect against clinical interest bacteria and bactericidal effect against phytopatogens. Conclusion. The results from physicochemical analysis reinforce the fact that it is imperative to include simple conventional methods in the analysis of oil products. The analysis of copaiba oil gives safe products and purity which ensure products with quality. Also, since copaiba oil is an over-the-counter product the results indicate that pharmacosurveillance must be improved by the governmental regulation agency to avoid microorganism resistance selection and to achieve better international quality products.
Wydawca
-
Rocznik
Tom
14
Numer
3
Opis fizyczny
p.215-225,fig.,ref.
Twórcy
autor
  • Biological Science Department, State University of Santa Cruz, 16 km Jorge Amado Road, 45662-900, Ilheus, Bahia, Brazil
autor
  • Pharmaceutical Science Graduate Program, Federal University of Rio Grande do Sul, 2752 Ipiranga Av., 90610-000, Porto Alegre, Rio Grande do Sul, Brazil
autor
  • Pharmaceutical Science Graduate Program, Federal University of Rio Grande do Sul, 2752 Ipiranga Av., 90610-000, Porto Alegre, Rio Grande do Sul, Brazil
  • Pharmaceutical Science Graduate Program, Federal University of Rio Grande do Sul, 2752 Ipiranga Av., 90610-000, Porto Alegre, Rio Grande do Sul, Brazil
  • Biological Science Department, State University of Santa Cruz, 16 km Jorge Amado Road, 45662-900, Ilheus, Bahia, Brazil
autor
  • Basic and Instrumental Studies Department, State University of Southeast Bahia, BR Road 415 03 Km, 45700-000, Itapetinga, Bahia, Brazil
autor
  • Basic and Instrumental Studies Department, State University of Southeast Bahia, BR Road 415 03 Km, 45700-000, Itapetinga, Bahia, Brazil
autor
  • Exact and Technological Sciences Department, State University of Santa Cruz, 16 km Jorge Amado Road, 45662-900, Ilheus, Bahia, Brazil
  • Biological Science Department, State University of Santa Cruz, 16 km Jorge Amado Road, 45662-900, Ilheus, Bahia, Brazil
Bibliografia
  • Adams, R. P. (2009). Identification of essential oil components by gas chromatography/mass spectrometry. Illinois: Allured.
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  • Barbosa, K. S., Yoshida, M., Scudeller, V. V. (2009). Detection of adulterated copaiba (Copaifera multijuga Hayne) oil-resins by refractive index and thin layer chromatography. Rev. Brasil. Farmac., 19, 1A, 57-60.
  • Barbosa, P. C. S., Medeiros, R. S., Sampaio, P. T. B., Vieira, G„ Wiedemann, L. S. M., V.-J., Valdir, F. (2012). Influence of abiotic factors on the chemical composition of copaiba oil (Copaifera multijuga Hayne): soil composition, seasonality and diameter at breast height. J. Braz. Chem. Soc., 23, 1823-1833.
  • Biavatti, M. W., Dossin, D., Deschamps, F. C., Lima, M. P. (2006). Análise de óleos-resinas de copaiba: contribuifao para o seu controle de qualidade [Copaiba oil-resin analysis: contribution to quality control]. Rev. Bras. Farmac., 16, 2, 230-235.
  • Correia, A. F., Segovia, J. F. O., Gonsalves, M. C. A., de Oliveira, V. L., Silveira, D., Carvalho, J. C. T., Kanzaki,
  • L. I. B. (2008). Amazonian plant crude extract screening for activity against multidrug-resistant bacteria. Eur. Rev. Med. Pharm. Sci., 12, 369-380.
  • Deus, R. J. A. D., Alves, C. N., Arruda, M. S. P. (2011). Evaluation of the antifungal effect of oleoresin and essential oil of copaiba (Copaifera multijuga Hayne). Braz. J. Med. Plants, 13, 1-7.
  • Estevao, L. R. M., Medeiros, J. P., Scognamillo-Szabo, M. V. R., Baratella-Evencio, L., Guimaraes, E. C., Camara, C. A. G., Evencio-Neto, J. (2009). Neoangiogenesis of skin flaps in rats treated with copaiba oil. Pesquisa Agro- pec. Brasil., 44, 406-412.
  • Leandro, L. M., Vargas, de F. S., Barbosa, P. C. S., Neves, J. K. O., da Silva, J. A., da Veiga-Junior, V. F. (2012). Chemistry and biological activities of terpenoids from copaiba (Copaifera spp.) oleoresins. Molecules, 17, 3866-3889.
  • Marchesini, A. M., Prado, G. G., Messiano, G. B., Machado, M. B., Lopes, L. M. X. (2009). Chemical constituents of Aristolochia giberti. J. Brazil. Chem. Soc., 20, 1598-1608.
  • Mendonfa, D. E., Onofre, S. B. (2009). Antimicrobial activity of the oil-resin produced by copaiba Copaifera multijuga Hayne (Leguminosae). Braz. J. Pharmac., 19, 2B, 577-581.
  • Messiano, G. B., Vieira, L., Machado, M. B., Lopes, L. M. X., de Bortoli, S. A., Zukerman-Schpector, J. (2008). Evaluation of insecticidal activity of diterpenes and lignans from Aristolochia malmeana against Anticarsia gemmatalis. J. Agric. Food Chem., 56, 2655-2659.
  • Oliveira, E. C. P., Lameira, O. A., Zoghbi, M. G. B. (2006). Identification of the time for collecting the oil-resin of copaiba (Copaifera spp.) in the city of Moju, Para state, Brazil. Braz. J. Med. Plants, 8, 14-23.
  • Pedroso, R. S., Menezes, R. P., Ferreira, J. C., Penatti, M. P. A., Sd, W. M., Malvino, L. D. S., Candido, R. C., Moreira, T. A. (2014). Antifungal susceptibility of isolates of Candida spp. by disk diffusion and broth microdilution. Biosci. J, 30, 1,304-311.
  • Pieri, F. A., Mussi, M. C., Moreira, M. A. S. (2009). Copaiba oil (Copaifera sp.): history, extraction, industrial applications and medicinal properties. Braz. J. Med. Plants, 11, 465-472.
  • Pieri, F. A., Mussi, M. C. M., Fiorini, J. E., Moreira, A. S., Schneedorf, J. M. (2012). Bacteriostatic effect of copaiba oil (Copaifera officinalis) against Streptococcus mutans. Brazil. Dent. J., 23, 36-38.
  • Santos, A. O., Ueda-Nakamura, T., Dias Filho, B. P., Veiga Jr., V. F., Pinto, A. C., Nakamura, C. V. (2008). Antimicrobial activity of Brazilian copaiba oils obtained from different species of the Copaifera genus. Memór. Inst. Oswaldo Cruz, 103, 277-281.
  • Sartoratto, A., Machado, A. L. M., Delarmelina, C., Figueira, G. M., Duarte, M. C. T., Rehder, V. L. G. (2004). Composition and antimicrobial activity of essential oils from aromatic plants used in Brasil. Brazil. J. Microbiol., 35,275-280.
  • Souza, A. B., Moreira, M. R., Borges, C. H. G., Simáo, M. R., Bastos, J. K., de Sousa, J. R B., ..., Veneziani, R. C. S. (2013). Development and validation of a rapid RP- HPLC method for analysis of (-)-copalic acid in copaiba oleoresin. Biomed. Chromat., 27, 280-283.
  • Tempone, A. G. Sartorelli, P., Teixeira, D., Prado, F. O., Calixto, I. A., Lorenzi, H., Melhem, M. S. C. (2008). Brazilian flora extracts as source of novel antileishmanial and antifungal compounds. Memôr. Inst. Oswaldo Cruz, 103, 443-449.
  • Vasconcelos, A. F. F., Godinho, O. E. S. (2002). Use of classical analytical methods to investigate the authenticity of copaiba oil. Quim. Nova, 25, 6/B, 1057-1060.
  • Veiga Jr., V. F., Patitucci, M. L., Pinto, A. C. (1997). Authenticity control of commercial copaiba oils by high resolution gas chromatography. QuimicaNova, 20, 612-615.
  • Veiga Jr., V. F., Pinto, A. C. (2002). The Copaifera L. genus. Quim. Nova, 25, 273-286.
  • Vieira, R. C., Bombardiere, E., Oliveira, J. J., Lino, J. R. R. S., Brito, L. A. B., Junqueira-Kipnis, A. P. (2008). Influence of Copaifera langsdorjjji oil on the repair of a surgical wound in the presence of foreign body. Brazil. J. Vet. Res., 28, 358-366.
Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
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