Glial expression of purinergic P2X7 receptor in rat brain during the course of experimental autoimmune encephalomyelitis

• Autorzy: Grygorowicz T. , Sulejczak D. , Sulkowski G. , Struzynska L.
• Języki publikacji: EN

Abstrakty

EN

Multiple sclerosis is a common neurodegenerative disease with prevalence in Poland about 15 per 10000 people. It is characterized by inflamed lesions in myelin sheaths surrounding axons in the white matter of the brain and spinal cord. These changes lead to the damage of axons and, in consequence, to a broad spectrum of neurological symptoms. Experimental autoimmune encephalomyelitis (EAE) is the well known and commonly used animal model of MS. In the present study the temporal pattern of glial activation (microglia and astroglia) together with P2X7R expression were investigated in brain of Lewis rats during the course of EAE. This receptor, activated under pathological conditions, may participate in the regulation of inflammatory response and cell death. It was shown to induce the release of inflammatory mediators like Il-1b and TNF-a in different types of glial cells. It is also pathologically involved in the release of potentially cytotoxic substances like glutamate and ATP. Western blot analysis was used to assess the relative concentration of P2X7R protein in glial fraction whereas its cellular localization was studied by immunohistochemical method. Tissue was labeled with the specific markers (Iba1 - microglial marker, GFAP - astroglial marker) and examined in the different stages post immunization (2, 4, 6, 8, 10 days). We observed the early overexpression of P2X7R protein (2-4 d.p.i.) in glial fraction obtained from brains of EAE rats with parallel enhancement of glial markers. Double immunofluorescent labeling showed colocalization of the receptor with glial markers. The results revealed that activation of both astroglia and microglia takes place very early post immunization, well before the neurological symptoms of the disease occur and is temporary connected with the overexpression of P2X7R. This suggests the involvement of P2X7R-mediated signals into the early pathological mechanisms operating during the disease.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: 1
• Opis fizyczny: p.162

Twórcy

autor

Grygorowicz T.

• Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Sulejczak D.

• Department of Experimental Pharmacology, Mossakowski Medical Research Centre Polish Academy of Sciences, Warsaw, Poland

autor

Sulkowski G.

• Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland

autor

Struzynska L.

• Laboratory of Pathoneurochemistry, Department of Neurochemistry, Mossakowski Medical Research Centre, Polish Academy of Sciences, Warsaw, Poland