Plant growth-promoting traits of phosphate solubilizing bacteria isolated from Hippophae rhamnoides L. (Sea-buckthorn) growing in cold desert Trans-Himalayan Lahul and Spiti regions of India

• Autorzy: Kumar A. , Guleria S. , Mehta P. , Walia A. , Chauhan A. , Shirkot C.K.
• Języki publikacji: EN

Abstrakty

EN

One hundred and six P-solubilizing bacteria (PSB) from rhizosphere soil (RS) and root endosphere (ER) of Hippophae rhamnoides L.(Sea-buckthorn) of two locations viz. Lahaul and Spiti of Himachal Pradesh were isolated using culture-dependent procedures and were screened for various plant growth-promoting traits (PGPTs). Indole acetic acid produced was detected in 76.41 % isolates, siderophore synthesis in 43.39 % isolates and hydrogen cyanide in 19.4 % isolates. The percent growth inhibition against Fusarium oxysporum and Alternaria sp. was detected in 25.5 and 47.2 % isolates, respectively. Overall, 23.56 % of PSB isolates from RS and 16.85 % from ER showed none of the PGPTs tested. Among the PSB showing PGPTs, 36.58 % had single trait and 43.08 % had multiple traits showing two (29.68 %) and three (13.82 %) types of PGPTs. The Shannon–Weaver diversity index revealed that the proportion of PSB isolates possessing PGPTs was higher for RS than for the ER. Furthermore, the five best performing PSBs under in vitro conditions were selected for pot experiment to evaluate their efficiency for growth promotion of tomato seedlings under net house conditions. Among five isolates, Bacillus subtilis CKS1 exhibited significant increase in shoot length (13.82 %), root length (25.07 %), shoot dry weight (29.47 %) and root dry weight (33.33 %). Plant growth-promoting and antifungal activities of Bacillus subtilis CKS1 depict that it may be utilized as a potential biofertilizer for tomato.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 03
• Opis fizyczny: Article: 48 [12 p.], fig.,ref.

Twórcy

autor

Kumar A.

• Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry Nauni, Solan 173230, H.P., India

autor

Guleria S.

• Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry Nauni, Solan 173230, H.P., India

autor

Mehta P.

• DBT-IOC Centre for Bioenergy Research, Indian Oil R and D Centre, Sector-13, Faridabal 121007, Haryana, India

autor

Walia A.

• Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry Nauni, Solan 173230, H.P., India

autor

Chauhan A.

• Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry Nauni, Solan 173230, H.P., India

autor

Shirkot C.K.

• Department of Basic Sciences (Microbiology Section), Dr. Y. S. Parmar University of Horticulture and Forestry Nauni, Solan 173230, H.P., India

Bibliografia

• Adesemoye AO, Torbert HA, Kloepper JW (2008) Enhanced plant nutrient use efficiency with PGPR and AMF in an integrated nutrient management system. Can J Microbiol 54:876–886
• Alstrom S, Burns RG (1989) Cyanide production by rhizobacteria as a possible mechanism of plant growth inhibition. BiolFertil Soils 7:232–238
• Babi AA, Anderson JD (1973) Vigour determination in soybean seed by multiple criteria. Crop Sci 13:630–633
• Baker, Schippers (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas sp. mediated plant growth stimulation. Soil Biol Biochem 19:451–457
• Barriuso J, Ramos Solano B, Santamaria C, Daza A, Gutierrez Manero FJ (2008) Effect of inoculum with putative plant growthpromoting rhizobacteria isolated from Pinus sp. on Pinuspinea growth, mycorrhization and rhizosphere microbial communities. J Appl Microbiol 105(5):1298–1309
• Dubeikovsky AN, Mordukhova EA, Kochetkov VV, Polikarpova FY, Boronin AM (1993) Growth promotion of blackcurrant softwood cuttings by recombinant strain Pseudomonas fluorescens BSP53a synthesizing an increased amount of indole-3-acetic acid. Soil Biol Biochem 25:1277–1281
• Felsenstein J (1993) PHYLIP (Phylogeny Inference Package) version 3.5c. Seattle (WA): Department of Genetics, University of Washington
• Glick BR (2012) Plant growth promoting bacteria: Mechanisms and Applications. Scientifica. doi:10.6064/2012/963401
• Gulati A, Rahi P, Vyas P (2008) Characterization of phosphatesolubilizing fluorescent Pseudomonads from the rhizosphere of seabuckthorn growing in the cold deserts of Himalayas. Curr Microbiol 56:73–79
• Herman MAB, Nault BA, Smart CD (2008) Effects of plant growth promoting rhizobacteria on bell pepper production and green peach aphid infestations in New York. Crop Prot 27:996–1002
• Higgins D, Thompson J, Gibson T, Thompson JD, Higgins DG, Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22:4673–4680
• Holt JG, Krieg NR, Sneathm PHA, Staley JT, Williams ST (1994) Bergey’s manual of determinative bacteriology, 9th edn. Williams and Williams, Baltimore
• Joshi P, Bhatt AB (2011) Characterization of rhizobacteria diversity isolated from Oryza sativa cultivated at different altitude in North Himalaya. Adv Appl Sci Res 2(4):208–216
• Koo SY, Hong SH, Ryu HW, Cho KS (2010) Plant growth promoting trait of rhizobacteria isolated from soil contaminated with petroleum and heavy metals. J Microbiol Biotechnol 20:587–593
• Kumar D, Shivay YS, Dhar S, Kumar C, Prasad R (2013) Rhizospheric flora and the influence of agronomic practices on them—a review. Proc Natl Acad Sci India Sect B Biol Sci 83(1):1–14
• Mehta P, Chauhan A, Mahajan R, Mahajan PK, Shirkot CK (2010) Strain of Bacillus circulansisolated from apple rhizosphere showing plant growth promoting potential. Curr Sci 98:538–542
• Mehta P, Walia A, Chauhan A, Shirkot CK (2013) Plant growth promoting traits of phosphate-solubilizing rhizobacteria isolated from apple trees in trans Himalayan region of Himachal Pradesh. Arch Microbiol. doi:10.1007/s00203-013-0881-y
• Mittal S, Johri BN (2007) Assessment of rhizobacterial diversity of Triticumaestivum and Eleusinecoracana from Northern region of India. Curr Sci 93(11):1530–1537
• Mohamed HI, Gomaa EZ (2012) Effect of plant growth promoting Bacillus subtilis and Pseudomonas fluorescens on growth and pigment composition of radish plants (Raphanussativus) under NaCl stress. Photosynthetica 50(2):263–272
• Pikovskaya RI (1948) Mobilization of phosphorus in soil in connection with the vital activity of some microbial species. Microbiologiya 7:362–370
• Ramos Solano B, García JAL, Villaraco AG, Algar E, Cristobal JG, Manero FJG (2010) Siderophore and chitinase producing isolates from the rhizosphere of Nicotianaglauca Graham enhance growth and induce systemic resistance in Solanumlycopersicum L. Plant Soil 334:189–197
• Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56
• Shishido MC, Breuil P, Christopher C (1999) Endophytic colonization of spruce by plant growth-promoting rhizobacteria. FEMS Microbiol Ecol 29:191–196
• Singh RP, Gupta MK (1990) Soil and vegetation study of Lahauland Spiti cold desert of Western Himalayas. Indian For 116:785–790
• Ventorino V, Chiurazzi M, Aponte M, Pepe O, Moschetti G (2007) Genetic diversity of a natural population of Rhizobium leguminosarumbv. Vicianodulating plants of Viciafaba in the Vesuvian area. Curr Microbiol 55:512–517
• Verma JP, Yadav J, Tiwari KN, Lavakush Singh V (2010) Impact of plant growth promoting rhizobacteria on crop production. Int J Agric Res 5:954–983
• Walia A, Mehta P, Chauhan A, Shirkot CK (2013) Effect of Bacillus subtilis strain CKT1 as inoculum on growth of tomato seedlings under net house conditions. Proc Natl Acad Sci India Sect B Biol Sci. doi:10.007/s40011-013-0189-3
• Warnock DW (1989) Methods with antifungal drugs. In: Evans EGV, Richardson MD (eds) Medical mycology-a practical approach. IEL Press, Oxford, pp 235–259

Identyfikatory

DOI

10.1007/s11738-014-1793-z