Role of Nigella sativa seed oil on corneal injury induced by formaldehyde in adult male albino rats

• Autorzy: Salem N.A. , Mahmoud O.M. , Al Badawi M.H. , Gab-Alla A.A.
• Języki publikacji: EN

Abstrakty

EN

Formaldehyde is frequently used in occupational environments, its toxicity concerns to all who work closely with it such as anatomists. Nigella sativa is an amazing herb with a rich historical and religious background; its seeds are the source of its active ingredients. The purpose of the present study was to evaluate the role of Nigella sativa oil on corneal injury induced by formaldehyde in adult male albino rats. Forty adult male albino rats were divided randomly to four groups 10 rats each: I — Control group, II — Formaldehyde exposed group, III — Nigella sativa oil group (40 mg/kg/day) via intragastric tube daily for 2 weeks and IV — Formaldehyde and Nigella sativa group. Rats were sacrificed with ether, the corneas were extracted, one processed for haematoxylin and eosin stain and the other was used for transmission electron microscopic examination. Our results in group II revealed marked disorganisation, erosion, vacuolation and necrosis of epithelial cells with loss of parts of epithelial layer. Large congested invasion of blood vessels with separation and disorganisation of stromal fibrils. The corneas of group IV showed intact layers of epithelial cells with appearance close to control group. Star shaped cells (limbal stem cells) were obviously noticed in basal and intermediate layer with intact Bowman’s membrane. Stroma showed regular parallel collagen, limbal stem cells were also noticed in group III. We concluded that Nigella sativa oil can ameliorate the toxic changes of formaldehyde on rat corneas. (Folia Morphol 2016; 75, 4: 518–526)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2016
• Tom: 75
• Numer: 4
• Opis fizyczny: p.518-526,fig.,ref.

Twórcy

autor

Salem N.A.

• Department of Anatomy, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

autor

Mahmoud O.M.

• Department of Anatomy, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

autor

Al Badawi M.H.

• Department of Anatomy, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

autor

Gab-Alla A.A.

• Department of Ophthalmology, Faculty of Medicine, Suez Canal University, Ismailia, Egypt

Bibliografia

• 1. Bashir MU, Qureshi HJ (2010) Analgesic effect of Nigella sativa seeds extract on experimentally induced pain in albino mice. J Coll Physicians Surg Pak, 20: 464–467.
• 2. Beyazyıldız E, Alpaslan F, Beyazyıldız O, Beden O, Delibaş T (2012) A modified rat model for corneal alkali burn. Niche, 1: 31–33.
• 3. Bourgou S, Pichette A, Marzouk B, Legault J (2012) Antioxidant, anti-inflammatory, anticancer and antibacterial activities of extracts from Nigella sativa (black cumin) plant parts. J Food Biochem, 36: 539–546.
• 4. Chopra RN, Chopra IC, Handa KL, Kapur LD (1982) Indigenous Drugs of India, Academic Publishers; Calcutta, New Delhi, India, p. 582.
• 5. Dua HS, Joseph A, Shanmuganathan VA, Jones RE (2003). Stem cell differentiation and the effects of deficiency. Eye (Lond), 17: 877–885.
• 6. Ebato B, Friend J, Thoft RA (1988).Comparison of limbal and peripheral human corneal epithelium in tissue culture. Invest Ophthalmol Vis Sci, 29, 1533–1537.
• 7. Fini ME (1999) Keratocyte and fibroblast phenotypes in the repairing cornea. Prog Retin Eye Res, 18: 529–551.
• 8. Ghosheh OA, Houdi AA, Crooks PA (1999) High performance liquid chromatographic analysis of the pharmacologically active quinones and related compounds in the oil of the black seed (Nigella sativa L.). J Pharm Biomed Anal, 19: 757–762.
• 9. Goreja WG (2003) Black Seed: Nature’s Miracle Remedy. Amazing Herbs Press, New York, NY7.
• 10. Hoenerhoff MJ, Hong HH, Ton TV, Lahousse SA, Sills RC (2009) A review of the molecular mechanisms of chemically induced neoplasia in rat and mouse models in National Toxicology Program bioassays and their relevance to human cancer. Toxicol Pathol, 37: 835–848.
• 11. Houghton PJ, Zarka R, de las Heras B, Hoult JR (1995) Fixed oil of Nigella sativa and derived thymoquinone inhibit eicosanoid generation in leukocytes and membrane lipid peroxidation. Planta Med, 61: 33–36.
• 12. Huang AJ, Tseng SC (1991) Corneal epithelial wound healing in the absence of limbal epithelium. Invest Ophthalmol Vis Sci, 32: 96–105.
• 13. Huffman MA (2003) Animal self-medication and ethnomedicine: exploration and exploitation of the medicinal properties of plants. Proc Nutr Soc, 62: 371–381.
• 14. IARC (2006) Formaldehyde, 2-butoxyethanol and 1-tertbutoxypropan- 2-ol.Monogr Eval Carcinog. Risks Hum, 88, 1–478.
• 15. Jester JV, Li L, Molai A, Maurer JK (2001) Extent of initial corneal injury as a basis for alternative eye irritation tests. Toxicol Vitro, 15: 115–130.
• 16. Kalus U, Pruss A, Bystron J, Jurecka M, Smekalova A, Lichius JJ, Kiesewetter H (2003) Effect of Nigella sativa (black seed) on subjective feeling in patients with allergic diseases. Phytother Res, 17: 1209–1214.
• 17. Kawakita T, Espana EM, He H, Li W, Liu CY, Tsenq SC (2005) Intrastromal invasion by limbal epithelial cells is mediated by epithelial-mesenchymal transition activated by air exposure. Am J Pathol, 167: 381–393.
• 18. Kawashima M, Kawakita T, Satake Y, Higa K, Shimazaki J (2007) Phenotypic study after cultivated limbal epithelial transplantation for limbal stem cell deficiency. Arch Ophthalmol, 125: 1337–1344.
• 19. Khazaei M, Salehi H (2006) Protective effect of falcaria vulgaris extract on ethanol induced gastric ulcer in rat. Iran J Pharmcol Ther, 5: 43–46.
• 20. Kriebel D, Myers D, Cheng M, Woskie S, Cocanour B (2001) Short-term effects of formaldehyde on peak expiratory flow and irritant symptoms. Arch Environ Health, 56: 11–18.
• 21. Lai L-J, Hsu W-H, Wu AM, Wu JH (2013) Ocular injury by transient formaldehyde exposure in a rabbit eye model. PLoS One, 8: e66649.
• 22. Lavker RM, Sun TT (2003) Epithelial stem cells: the eye provides a vision. Eye, 17: 937–942.
• 23. Mann I (1944) A study of epithelial regenration in the living eye. Br J Ophthalmol, 28: 26–40.
• 24. Maurer JK, Molai A, Parker RD, Li LI, Carr GJ, Petroll WM, Cavanagh HD, Jester JV (2001) Pathology of ocular irritation with acetone, cyclohexanol, parafluoroaniline, and formaldehyde in the rabbit low-volume eye test. Toxicol Pathol, 29: 187–199.
• 25. Nakazawa H, Ikeda H, Yamashita T, Hara I, Kumai Y, Endo G, Endo Y (2005) A case of sick building syndrome in a Japanese office worker. Ind Health, 43: 341–345.
• 26. Nagi MN, Mansour MA (2000) Protective effect of thymoquinone against doxorubicin-induced cardiotoxicity in rats: a possible mechanism of protection. Pharmacol Res, 41: 283–289.
• 27. Pellegrini G, Golisano O, Paterna P, Lambiase A, Bonini S, Rama P, De Luca M (1999) Location and clonal analysis of stem cells and their differentiated progeny in the human ocular surface. J Cell Biol, 145: 769–782.
• 28. Recio L, Sisk S, Pluta L, Bermudez E, Gross EA, Chen Z, Morgan K, Walker C (1992) P53 Mutations in formaldehydeinduced nasal squamous cell carcinomas in rats. Cancer Res, 52: 6113–6116.
• 29. Rolando M, Zierhut M (2001) The ocular surface and tear film and their dysfunction in dry eye disease. Surv Ophthalmol, 45: S203–S210.
• 30. Saika S, Yamanaka O, Sumioka T, Miyamoto T, Miyazaki K, Okada Y, Kitano A, Shirai K, Tanaka S, Ikeda K (2008) Fibrotic disorders in the eye: targets of gene therapy. Prog Retin Eye Res, 27: 177–196.
• 31. Salem ML (2005) Immunomodulatory and therapeutic properties of the Nigella sativa L. seed. International Immunopharmacology, 5: 1749–1770
• 32. Solomons K, Cochrane JW (1984) Formaldehyde toxicity. Part II. Review of acute and chronic effects on health. S Afr Med J, 66: 103–106.
• 33. Suryavanshi VL, Sathe PA, Baing MM, Singh GR, Lakshmi SN (2007) Determination of Rutin in Amaranthus spinosus Linn. Whole Plant Powder by HPTLC. Int Immunopharmacol, 5: 1749–1770.
• 34. Tang XQ, Ren YK, Chen RQ, Zhuang YY, Fang HR, Xu JH, Wang CY, Hu B (2011) Formaldehyde induces neurotoxicity to PC12 cells involving inhibition of paraoxonase-1 expression and activity. Clin Exp Pharmacol Physiol, 38: 208–214.
• 35. Tanko Y, Mohammed A, Okasha MA, Shuaibu A, Magaji MG, Yaro AH (2007) Analgesic and anti-inflammatory activities of ethanol seed extract of Nigella sativa (black cumin) inmice and rats. Eur J Sci Res, 18: 277–281.
• 36. Tseng SC, Li DQ (1996) Comparison of protein kinase C subtype expression between normal and aniridic human ocular surfaces: implications for limbal cell dysfunction in aniridia. Cornea, 15: 168–178.
• 37. Welge-Lussen, U, May CA, Neubauer AS, Priglinger S (2001) Role of tissue growth factors in aqueous humor homestasis. Curr Opin Ophthalmol, 12: 94–99.
• 38. West-Mays JA, Dwivedi DJ (2006) The keratocyte: corneal stromal cell with variable repair phenotypes. Int J Biochem Cell Biol, 38: 1625–1631.
• 39. Zwart A, Woutersen RA, Wilmer JW, Spit BJ, Feron VJ (1988) Cytotoxic and adaptive effects in rat nasal epithelium after 3-day and 13-week exposure to low concentrations of formaldehyde vapour. Toxicology, 51: 87–99.

Identyfikatory

DOI

10.5603/FM.a2016.0010