Identification of main virulence markers of food-borne pathogens recovered from bovine carcasses as an aid in assessing public health risk

• Autorzy: Wieczorek K. , Kania I. , Osek J.
• Języki publikacji: EN

Abstrakty

EN

The prevalence of potentially pathogenic Campylobacter sp., L. monocytogens, and VTEC strains through the detection of their virulence markers was estimated by the PCR method. The presence of eleven genes was detected in C. jejuni and C. coli strains. The majority of the isolates showed the cadF, cdtA, cdtB, cdtC,flhA, ciaB, docA, flaA, and iam genes, whereas wlaN was amplified in one isolate and virB11 gene was not found among the tested Campylobacter strains. Two stx2 Shiga toxin genes - stx2e and stx2d gene subtypes were observed among the VTEC strains examined. Multiplex PCR was performed to discriminate four major L. monocytogenes serovars. The majority (five of seven strains) of the Listeria isolates were identified as the 1/2a serovar, followed by l/2c (two strains). These data suggest that the bacteria recovered from bovine carcasses are potentially pathogenic for humans and may have important public health implications.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2009
• Tom: 53
• Numer: 3
• Opis fizyczny: p.425-432,ref.

Twórcy

autor

Wieczorek K.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Kania I.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

autor

Osek J.

• Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

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