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2016 | 160 | 12 |

Tytuł artykułu

Ligninolityczne właściwości izolatów Heterobasidion parviporum w hodowli na drewnie świerka

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Treść / Zawartość

Warianty tytułu

EN
Ligninolytic activity of Heterobasidion parviporum isolates in cultivation on Norway spruce wood

Języki publikacji

PL

Abstrakty

EN
The aim of this preliminary study was to assess the activity of laccase, diverse peroxidases as well as the level of micromolecular compounds in Heterobasidion parviporum isolates grown on pieces of Norway spruce wood (sapwood and heartwood) during 50 days of incubation under the laboratory conditions. H. parviporum isolates secreted extracellular enzymes: laccase, manganese peroxidase (MnP), lignin peroxidase (LiP) and versatile peroxidase (VP). Hydroxy− and methoxyphenols were also released during this process. The above−mentioned enzymes showed low activity in mycelium grown on both sapwood and heartwood. The activity of laccase ranged from 0 to 0.513 mU/mg protein on sapwood, and from 0 to 0.106 mU/mg protein – on heartwood. MnP activity of H. parviporum isolates ranged from 0.024 to 0.667 mU/mg protein on sapwood, and from 0.038 to 1.585 mU/mg protein – on heartwood. LiP activity was small and ranged from 0 to 1.281 mU/mg protein on sapwood, and from 0.013 to 0.166 mU/mg protein – on heartwood. Activity of VP oxidizing manganese ions was low. It ranged from 0 to 3.063 mU/mg protein on sapwood, and from 0.059 to 3.054 mU/mg protein – on heartwood. The activity of VP oxidizing guaiacol ranged from 0.006 to 1.490 mU/mg protein on sapwood and from 0.038 to 1.147 mU/mg protein – on heartwood. The hydroxyphenols produced by H. parviporum isolates ranged from 15.037 to 110.149 mg of protocatechuic acid/ml on sapwood, and from 11.236 to 27.220 mg of protocatechuic acid/ml – on heartwood. Methoxyphenols produced by H. parviporum isolates ranged from 3.393 to 24.253 mg of vanillic acid/ml on sapwood, and from 4.955 to 12.005 mg of vanillic acid/ml – on heartwood.

Wydawca

-

Czasopismo

Rocznik

Tom

160

Numer

12

Opis fizyczny

s.1027-1035,rys.,bibliogr.

Twórcy

autor
  • Zakład Ochrony Lasu, Instytut Badawczy Leśnictwa, Sękocin Stary, ul.Braci Leśniej 3, 05-090 Raszyn
autor
  • Uniwersytet Przyrodniczy w Lublinie, ul.St.Leszczyńskiego 7, 20-069 Lublin

Bibliografia

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Bibliografia

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