Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 36 | 02 |
Tytuł artykułu

OsCPK20 positively regulates Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against Magnaporthe grisea

Warianty tytułu
Języki publikacji
Calcium-dependent protein kinases are important decoders of calcium signals in plants, which are involved in plant immunity. We report isolation and functional characterization of a pathogen-responsive OsCPK20 gene in rice. The expression of OsCPK20 in rice was significantly induced following treatment with a Magnaporthe grisea elicitor. Overexpression of constitutively active OsCPK20 in Arabidopsis enhanced the resistance to infection with Pseudomonas syringae pv. tomato, associated with elevated expression of both SAand JA-related defense genes. Similarly, transgenic rice plants containing constitutively active OsCPK20 exhibited enhanced resistance to blast fungus M. grisea. The enhanced resistance in the transgenic Arabidopsis and rice was associated with activated expression of both SA- and JA-related defense genes. We also found that OsCPK20 was significantly induced by drought stress, indicating that OsCPK20 might be involved in plant response to drought stress. Taken together, our results indicate that rice OsCPK20 positively regulates Arabidopsis resistance against Pseudomonas syringae pv. tomato and rice resistance against M. grisea, and that it may enhance disease resistance by activating both SA- and JA-dependent defense responses.
Słowa kluczowe
Opis fizyczny
  • State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China
  • State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China
  • State Key Laboratory of Protein and Plant Gene Research, College of Life Sciences, Peking University, Beijing, 100871, China
  • Agrawal GK, Rakwal R, Jwa N-S (2000) Rice (Oryza sativa L.) OsPR1b gene is phytohormonally regulated in close interaction with light signals. Biochem Biophys Res Commun 278:290–298.
  • Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ (1990) Basic local alignment search tool. J Mol Biol 215:403–410.
  • Asano T, Hayashi N, Kobayashi M, Aoki N, Miyao A, Mitsuhara I, Ichikawa H, Komatsu S, Hirochika H, Kikuchi S, Ohsugi R (2012) A rice calcium-dependent protein kinase OsCPK12 oppositely modulates salt-stress tolerance and blast disease resistance. Plant J 69:26–36.
  • Boudsocq M, Willmann MR, McCormack M, Lee H, Shan L, He P, Bush J, Cheng S-H, Sheen J (2010) Differential innate immune signalling via Ca²⁺ sensor protein kinases. Nature 464:418.
  • Carrington JC, Freed DD (1990) Cap-independent enhancement of translation by a plant potyvirus 50 nontranslated region. J Virol 64:1590–1597.
  • Cheng S-H, Willmann MR, Chen H-C, Sheen J (2002) Calcium signaling through protein kinases. The Arabidopsis calcium-dependent protein kinase gene family. Plant Physiol 129: 469–485.
  • Coca M, San Segundo B (2010) AtCPK1 calcium-dependent protein kinase mediates pathogen resistance in Arabidopsis. Plant J 63:526–540.
  • Harmon AC, Gribskov M, Gubrium E, Harper JF (2001) The CDPK superfamily of protein kinases. New Phytol 151:175–183.
  • Harper JF, Harmon A (2005) Plants, symbiosis and parasites: a calcium signalling connection. Nat Rev Mol Cell Biol 6:555–566.
  • Harper JF, Huang J-F, Lloyd SJ (1994) Genetic identification of an autoinhibitor in CDPK, a protein kinase with a calmodulin-like domain. Biochemistry 33:7267–7277.
  • Harper JE, Breton G, Harmon A (2004) Decoding Ca²⁺ signals through plant protein kinases. Annu Rev Plant Biol 55:263–288.
  • He S, Tan G, Liu Q, Huang K, Ren J, Zhang X, Yu X, Huang P, An C (2011) The LSD1-interacting protein GILP is a LITAF domain protein that negatively regulates hypersensitive cell death in Arabidopsis. PLoS One 6:e18750.
  • Hrabak EM, Chan CWM, Gribskov M, Harper JF, Choi JH, Halford N, Kudla J, Luan S, Nimmo HG, Sussman MR, Thomas M, Walker-Simmons K, Zhu JK, Harmon AC (2003) The Arabidopsis CDPK-SnRK superfamily of protein kinases. Plant Physiol 132:666–680.
  • Jia Y, Valent B, Lee FN (2003) Determination of host responses to Magnaporthe grisea on detached rice leaves using a spot inoculation method. Plant Dis 87:129–133.
  • Kobayashi M, Ohura I, Kawakita K, Yokota N, Fujiwara M, Shimamoto K, Doke N, Yoshioka H (2007) Calcium-dependent protein kinases regulate the production of reactive oxygen species by potato NADPH oxidase. Plant Cell 19:1065–1080.
  • Kobayashi M, Yoshioka M, Asai S, Nomura H, Kuchimura K, Mori H, Doke N, Yoshioka H (2012) StCDPK5 confers resistance to late blight pathogen but increases susceptibility to early blight pathogen in potato via reactive oxygen species burst. New Phytol 196:223–237.
  • Koch E, Slusarenko A (1990) Arabidopsis is susceptible to infection by a downy mildew fungus. Plant Cell 2:437–445.
  • Lecourieux D, Raneva R, Pugin A (2006) Calcium in plant defence-signalling pathways. New Phytol 171:249–269.
  • Lee DH, Choi HW, Hwang BK (2011) The pepper E3 ubiquitin ligase RING1 gene, CaRING1, is required for cell death and the salicylic acid-dependent defense response. Plant Physiol 156:2011–2025.
  • Li J, Brader G, Kariola T, Palva ET (2006) WRKY70 modulates the selection of signaling pathways in plant defense. Plant J 46:477–491.
  • Liu XQ, Bai XQ, Qian Q, Wang XH, Chen MS, Chu CC (2005) OsWRKY03, a rice transcriptional activator that functions in defense signaling pathway upstream of OsNPR1. Cell Res 15:593–603.
  • Lorenzo O, Piqueras R, Sánchez-Serrano JJ, Solano R (2003) ETHYLENE RESPONSE FACTOR1 integrates signals from ethylene and jasmonate pathways in plant defense. Plant Cell 15:165–178.
  • Lu J, Ju H, Zhou G, Zhu C, Erb M, Wang X, Wang P, Lou Y (2011) An EAR-motif-containing ERF transcription factor affects herbivore-induced signaling, defense and resistance in rice. Plant J 68:583–596.
  • Murillo I, Jaeck E, Cordero MJ, San Segundo B (2001) Transcriptional activation of a maize calcium-dependent protein kinase gene in response to fungal elicitors and infection. Plant Mol Biol 45:145–158.
  • Peng YL, Shishiyama J (1988) Temporal sequence of cytological events in rice leaves infected with Pyricularia oryzae. Can J Bot Rev Can Bot 66:730–735.
  • Qiu D, Xiao J, Ding X, Xiong M, Cai M, Cao Y, Li X, Xu C, Wang S (2007) OsWRKY13 mediates rice disease resistance by regulating defense-related genes in salicylate- and jasmonate-dependent signaling. Mol Plant Microbe Interact 20:492–499.
  • Rakwal R, Agrawal GK, Agrawal VP (2001) Jasmonate, salicylate, protein phosphatase 2A inhibitors and kinetin up-regulate OsPR5 expression in cut-responsive rice (Oryza sativa). J Plant Physiol 158:1357–1362.
  • Romeis T, Piedras P, Jones JDG (2000) Resistance gene-dependent activation of a calcium-dependent protein kinase in the plant defense response. Plant Cell 12:803–815.
  • Romeis T, Ludwig AA, Martin R, Jones JDG (2001) Calciumdependent protein kinases play an essential role in a plant defence response. EMBO J 20:5556–5567.
  • Shah J (2003) The salicylic acid loop in plant defense. Curr Opin Plant Biol 6:365–371.
  • Shan X-Y, Wang Z-L, Xie D (2007) Jasmonate signal pathway in Arabidopsis. J Integr Plant Biol 49:81–86.
  • Sheen J (1996) Ca²⁺-dependent protein kinases and stress signal transduction in plants. Science 274:1900–1902.
  • Tsai T-M, Chen Y-R, Kao T-W, Tsay W-S, Wu C-P, Huang D-D, Chen W-H, Chang C-C, Huang H-J (2007) PaCDPK1, a gene encoding calcium-dependent protein kinase from orchid, Phalaenopsis amabilis, is induced by cold, wounding, and pathogen challenge. Plant Cell Rep 26:1899–1908.
  • Wan B, Lin Y, Mou T (2007) Expression of rice Ca²⁺-dependent protein kinases (CDPKs) genes under different environmental stresses. FEBS Lett 581:1179–1189.
  • Wang L, An C, Qian W, Liu T, Li J, Chen Z (2004) Detection of the putative cis-region involved in the induction by a Pyricularia oryzae elicitor of the promoter of a gene encoding phenylalanine ammonia-lyase in rice. Plant Cell Rep 22:513–518.
  • Xiong LZ, Yang YN (2003) Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell 15:745–759.
  • Xu J, Tian Y-S, Peng R-H, Xiong A-S, Zhu B, Jin X-F, Gao F, Fu X-Y, Hou X-L, Yao Q-H (2010) AtCPK6, a functionally redundant and positive regulator involved in salt/drought stress tolerance in Arabidopsis. Planta 231:1251–1260.
  • Yang GX, Shen SH, Yang SH, Komatsu S (2003) OsCDPK13, a calcium-dependent protein kinase gene from rice, is induced in response to cold and gibberellin. Plant Physiol Biochem 41: 369–374.
  • Zhang XR, Henriques R, Lin SS, Niu QW, Chua NH (2006) Agrobacterium-mediated transformation of Arabidopsis thaliana using the floral dip method. Nat Protoc 1:641–646.
  • Zhang X, Wang C, Zhang Y, Sun Y, Mou Z (2012) The Arabidopsis mediator complex subunit16 positively regulates salicylate-mediated systemic acquired resistance and jasmonate/ethylene-induced defense pathways. Plant Cell 24:4294–4309.
  • Zhu S-Y, Yu X-C, Wang X-J, Zhao R, Li Y, Fan R-C, Shang Y, Du S-Y, Wang X-F, Wu F-Q, Xu Y-H, Zhang X-Y, Zhang D-P (2007) Two calcium-dependent protein kinases, CPK4 and CPK11, regulate abscisic acid signal transduction in Arabidopsis. Plant Cell 19:3019–3036.
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.