Antioxidative system's responses in the leaves of six Caragana species dueing drought stress and recovery

• Autorzy: Kang H.-M. , Chen K. , Bai J. , Wang G.
• Języki publikacji: EN

Abstrakty

EN

For investigating the protective roles of antioxidative system in desiccation tolerance of Caragana species as they adapt to arid environments, we monitored a variety of ecophysiological parameters in the leaves of Caragana arborescens (mesophyte), C. microphylla (semiarid species), C. roborovskyi, C. stenophylla, C. acanthophylla, and C. tragacanthoides (xerophyte) grown under a drying-rehydration cycle. Relative leaf water content and chlorophyll content were decreased by 17.4–39.2 %, and by 14–40 %, respectively, after exposure to 48 days of drought stress. Malondialdehyde did not increase in xeric Caragana species. Hydrogen peroxide concentrations increased by 13.1–43.9 % except in C. acanthophylla. The activities of superoxide dismutase (SOD), guaiacol peroxidase (POD), ascorbate peroxidase (APX), glutathione reductase (GR) and reduced glutathione (GSH) in xeric Caragana species were significantly elevated with progressing drought stress. However, catalase in all species decreased markedly before drought stress treatment reached 40 days. The xeric Caragana species showed higher SOD, POD, APX, and GR activities, as well as ascorbate content, and more manganese SOD isoenzymes. C. arborescens and C. microphylla accumulated more free proline. Our data indicate that SOD and POD with the ascorbate–glutathione cycle have important protective effects in xeric Caragana species under drought stress. Free proline may be crucial in the resistance of C. arborescens and C. microphylla to drought stress.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 6
• Opis fizyczny: p.2145-2154,fig.,ref.

Twórcy

autor

Kang H.-M.

• Key Laboratory of Arid and Grassland Agroecology of Ministry of Lanzhou University, 730000 Lanzhou, People's of Republic of China
• Institute of Biology, Gansu Academy of Sciences, 730000 Lanzhou, People,s Republic of China

autor

Chen K.

• Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, People's Republic of China

autor

Bai J.

• College of Life Sciences, Northwest A&F University, Xi'an 712100, People's Republic of China

autor

Wang G.

• Key Laboratory of Arid and Grassland Agroecology of Ministry of Lanzhou University, 730000 Lanzhou, People's of Republic of China

Bibliografia

• Aebi HE (1983) Catalase. In: Bergmeyer HU (ed) Methods of enzymatic analysis, vol 3., Verlag ChmieWeiheim, German, pp 273–282
• Alia P, Mohanty P, Matysik J (2001) Effect of proline on the production of singlet oxygen. Amino Acids 21:195–200
• Alscher RG, Donahue JL, Cramer CA (1997) Reactive oxygen species and antioxidants: relationships in green plant cells. Physiol Plant 100:224–233
• Asada K (1999) The water–water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 50:601–639
• Bai J, Gong CM, Chen K, Kang HM, Wang G (2009) Examination of antioxidative system’s responses in the different phases of drought stress and during recovery in desert plant Reaumuria soongorica (Pall.) Maxim. J Plant Biol 52:417–425
• Barrs HD, Weatherley PE (1962) A re-examination of the relative turgidity technique for estimating water deficits in leaves. Aust J Biol Sci 15:413–428
• Bates LS, Waldren RP, Teare ID (1973) Rapid determination of free proline for water-stress studies. Plant Soil 39:205–207
• Beauchamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
• Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248–254
• Chai TT, Fadzillah NM, Kusnan M, Mahmood M (2005) Water stress-induced oxidative damage and antioxidant responses in micropropagated banana plantlets. Biol Plant 49:153–156
• Chance B, Maehly AC (1955) Assay of catalase and peroxidases. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 2. Acadamic Press, New York, pp 764–775
• Chen JX, Wang XF (2002) Experimental guidance in plant physiology. South China University of Technology Press, Guangzhou, pp 125–127 (in Chinese)
• Close TJ (1996) Dehydrins: emergence of a biochemical role of a family of plant dehydration proteins. Physiol Plant 97:795–803
• Feierabend J, Engel S (1986) Photoinactivation of catalase in vitro and in leaves. Arch Biochem Biophys 251:567–576
• Foyer CH, Noctor G (2000) Tansley Review 112. Oxygen processing in photosynthesis: regulation and signaling. New Phytol 146:359–388
• Fridovich I (1986) Superoxide dismutases. In: Meister A (ed) Advances in enzymology and related areas of molecular biology, vol 58. Wiley, New York, pp 61–97
• Giannopolities CN, Ries SK (1977) Superoxide dismutase. I. Occurrence in higher plants. Plant Physiol 59:309–314
• Grace SC, Logan BA (1996) Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species. Plant Physiol 112:1631–1640
• Heath RL, Packer L (1968) Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125:189–198
• Hernández JA, Ferrer MA, Jiménez A, Barceló AR, Sevilla F (2001) Antioxidant systems and O₂⁻ /H₂O₂ production in the apoplast of pea leaves. Its relation with salt-induced necrotic lesions in minor veins. Plant Physiol 127:817–831
• Hodges DM, DeLong JM, Forney CF, Prange RK (1999) Improving the thiobarbituric acid-reactive substances assay for estimating lipid peroxidation in plant tissues containing anthocyanin and other interfering compounds. Planta 207:604–611
• Hsiao TC (1973) Plant response to water stress. Annu Rev Plant Physiol 24:519–570
• Iannucci A, Russo M, Arena L, Fonzo ND, Martiniello P (2002) Water deficit effects on osmotic adjustment and solute accumulation in leaves of annual clovers. Eur J Agron 16:111–122
• Jiang YW, Huang BR (2001) Drought and heat stress injury to two cool-season turfgrasses in relation to antioxidant metabolism and lipid peroxidation. Crop Sci 41:436–442
• Jung S (2004) Variation in antioxidant metabolism of young and mature leaves of Arabidopsis thaliana subjected to drought. Plant Sci 166:459–466
• Lichtenthaler HK (1987) Chlorophylls and carotenoids—pigments of photosynthetic biomembranes. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol 148. Academic Press, San Diego, pp 350–382
• Ma CC, Gao YB, Wang JL (2004a) Ecological adaptation of Caragana opulens on the Inner Mongolia plateau: photosynthesis and water metabolism. Acta Phytoecol Sin 28:305–311 (in Chinese)
• Ma CC, Gao YB, Wang JL (2004b) The comparison studies of photosynthetic characteristics and protective enzymes of Caragana microphylla and Caragana stenophylla. Acta Ecol Sin 24:1594–1601 (in Chinese)
• Ma CC, Gao YB, Guo HY, Wang JL, Wu JB, Xu JS (2008) Physiological adaptation of four dominant Caragana species in the desert region of the Inner Mongolia Plateau. J Arid Environ 72:247–254
• Mittler R (2002) Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7:405–410
• Möller IM (2001) Plant mitochondria and oxidative stress: electron transport, ADPH turnover, and metabolism of reactive oxygen species. Annu Rev plant Physiol Plant Mol Biol 52:561–591
• Nakano Y, Asada K (1981) Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplast. Plant Cell Physiol 22:867–880
• Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279
• Pan Y, Wu LJ, Yu ZL (2006) Effect of salt and drought stress on antioxidant enzymes activities and SOD isoenzymes of liquorice (Glycyrrhiza uralensis Fisch). Plant Growth Regul 49:157–165
• Parida AK, Dagaonkar VS, PhalakMS,UmalkarGV, Aurangabadkar LP (2007) Alterations in photosynthetic pigments, protein and osmotic components in cotton genotypes subjected to short-term drought stress followed by recovery. Plant Biotechnol Rep 1:37–48
• Patterson BD, MacRae EA, Ferguson IB (1984) Estimation of hydrogen peroxide in plant extracts using titanium (IV). Anal Biochem 139:487–492
• Polle A (1997) Defense against photooxidative damage in plants. In: Scandalios JG (ed) Oxidative stress and the molecular biology of antioxidant defense. Cold Spring Harbor Laboratory Press, New York, pp 623–666
• Proctor MCF, Tuba Z (2002) Poikilohydry and homoihydry: antithesis or spectrum of possibilities?New Phytol 156:327–349
• Radotic K, Ducic T, Mutavdzic D (2000) Changes in peroxidase activity and isozymes in spruce needles after exposure to different concentrations of cadmium. Environ Exp Bot 44:105–113
• Ren J, Tao L, Liu XM (2002) Effect of different microhabitats and stand age on survival of introduced sand-fixing plants. J Arid Environ 51:413–421
• Ruth GA, Neval E, Lenwood SH (2002) Role of superoxide dismutases (SODs) in colltrolling oxidative stress in plants. J Exp Bot 53:1331–1341
• Seevers PM, Daly JM, Catedral FF (1971) The role of peroxidase isozymes in resistance to wheat stem rust disease. Plant Physiol 48:353–360
• Silveira JAG, Viégas RA, Rocha IMA, Moreira ACOM, Moreiro RA (2003) Proline accumulation and glutamine synthetase activity are increased by salt-induced proteolysis in cashew leaves. J Plant Physiol 160:115–123
• Sinhababu A, Kumar Kar R (2003) Comparative responses of three fuel wood yielding plants to PEG-induced water stress at seedling stage. Acta Physiol Plant 25:403–409
• Smirnoff N (1993) The role of active oxygen in the response of plants to water deficit and desiccation. New Phytol 125:27–58
• Sofo A, Tuzio AC, Dichio B, Xiloyannis C (2005) Influence of water deficit and rewatering on the components of the ascorbateglutathione cycle in four interspecific Prunus hybrids. Plant Sci 169:403–412
• Tűrkan İ, Bor M, Özdemir F, Koca H (2005) Differential responses of lipid peroxidation and antioxidants in the leaves of droughttolerant P. acutifolius Gray and drought-sensitive P. vulgaris L. subjected to polyethylene glycol mediated water stress. Plant Sci 168:223–231
• Vaidyanathan H, Sivakumar P, Chakrabarty R, Thomas G (2003) Scavenging of reactive oxygen species in NaCl-stressed rice (Oryza sativa L.)-differential response in salt-tolerant and sensitive varieties. Plant Sci 165:1411–1418
• Wang FZ, Wang QB, Kwonb SY, Kwakb SS, Sua WA (2005) Enhanced drought tolerance of transgenic rice plants expressing a pea manganese superoxide dismutase. J Plant Physiol 162:465–472
• Woodbury W, Spencer AK, Stahmann MA (1971) An improved procedure using ferricyanide for detecting catalase isozymes. Anal Biochem 44:301–305
• Yan L, Li H, Liu Y (2002) The anatomical ecology studies on the leaf of 13 species in Caragana genus. J Arid Land Res Environ 16:100–106 (in Chinese)
• Zgallaï H, Steppe K, Lemeur R (2006) Effects of different levels of water stress on leaf water potential, stomatal resistance, protein and chlorophyll content and certain anti-oxidative enzymes in tomato plants. J Integr Plant Biol 48:679–685
• Zhang ML (1998) A Preliminary Analytic Biogeography in Caragana (Fabaceae). Acta Bot Yunnan 20:1–11 (in Chinese)
• Zlatev ZS, Lidon FC, Ramalho JC, Yordanov IT (2006) Comparison of resistance to drought of three bean cultivars. Biol Plant 50:389–394

Uwagi

Rekord w opracowaniu