Expression of Kiss-1/GPR54 mRNA in the hypothalamic-pituitary -gonadalaxis in obese and diabetic male rats

• Autorzy: Gawalek M. , Kolodziejski P. , Pruszynska-Oszmalek E. , Kaczmarek P. , Sliwowska J. K.
• Języki publikacji: EN

Abstrakty

EN

Obesity is now dramatically on the rise and is a major risk factor for diabetes. Besides primary metabolic health problems occurring in people with obesity and diabetes there are numerous secondary problems including disruptions of the reproductive system. Kisspeptins and its receptor GPR54 play a key role in regulation of reproduction and integration of metabolic and reproductive systems. We hypothesized that obese and/or diabetic male rats would have altered Kiss-1 and/or GPR54 mRNA levels in the hypothalamic-pituitary-gonadal (HPG) axis. Rats were fed with high fat diet (HFD) for 5 weeks to induce obesity (DIO group). Injections of STZ were performed to induce diabetes type 1 (STZ group) or diabetes type 2 (HFD/STZ group). Control animals (C group) were fed with lab chow diet. Real-time PCR was performed. We have found that: (1) Kiss-1 and GPR54 expression in HPG axis was related to the rat metabolic status; (2) both STZ and HFD/STZ rats had elevated GPR54 mRNA level in the hypothalamus and (3) STZ rats had decreased Kiss-1 mRNA levels in the pituitary and decreased GPR54 levels in the testis. We have concluded that observed changes may contribute to reproductive failure in animals with diabetes. Supported by grant NCN 2011/01/B/NZ4/04992.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2013
• Tom: 73
• Numer: Suppl.1
• Opis fizyczny: p.26

Twórcy

autor

Gawalek M.

• Laboratory of Neurobiology, Institute of Zoology, Poznan University of Life Sciences, Poznan, Poland

autor

Kolodziejski P.

• Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Poznan, Poland

autor

Pruszynska-Oszmalek E.

• Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Poznan, Poland

autor

Kaczmarek P.

• Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, Poznan, Poland

autor

Sliwowska J. K.

• Laboratory of Neurobiology, Institute of Zoology, Poznan University of Life Sciences, Poznan, Poland