A novel pig gene, CPNE1, differentially expressed in the muscle tissues from Wujin pigs and Large White Pigs

• Autorzy: Shifei P. , Chuisi K. , Yonggang L.
• Języki publikacji: EN

Abstrakty

EN

The mRNA differential display technique was performed to investigate gene expression differences in the longissimus dorsi muscle from Wujin and Large White pigs. A fragment of one differentially expressed gene was isolated and sequenced. A complete cDNA sequence of the gene was obtained using the rapid amplification of cDNA ends (RACE) method. The open reading frame of this gene encodes a protein of 537 amino acids, which is homologous with the Copine I (CPNE1) of eight species: greater horseshoe bat (95%), mouse (92% ), rat (93%), cattle (95%), sheep (95%), rabbit (94%), human (95%) and dog (94%). This newly identified gene was respectively defined as the swine CPNE1 gene and had been assigned GeneID: 100233174. The phylogenetic analysis revealed that the swine CPNE1 gene has a closer genetic relationship with the CPNE1 gene of a dog. The tissue expression analysis indicated that the swine CPNE1 gene has a broad tissue distribution. The experiment described is the first to establish the primary foundation for further research on the swine CPNE1 gene.

• Wydawca: -
• Czasopismo: Animal Science Papers and Reports
• Rocznik: 2012
• Tom: 30
• Numer: 2
• Opis fizyczny: p.131-138,fig.,ref.

Twórcy

autor

Shifei P.

• Department of Animal Husbandry and Veterinary, Yunnan Vocational and Technical College of Agriculture, Kunming 650212, China

autor

Chuisi K.

autor

Yonggang L.

Bibliografia

• LIANG P., AVERBOUKH L., PARDEE A.B., 1993 – Distribution and cloning of eukaryotic mRNAs by means of differential display: Refinements and optimization. Nucleic Acids Research 21(14),3269-3275.
• LIANG P., PARDEE A.B., 1992 – Differential display of eukaryotic messenger RNA by means of polymerase chain reaction. Science 257(5072), 967-971.
• LIU G.Y., 2009 – A novel HADHA gene differentially expressed in muscle and other tissues from black-boned vs. ordinary sheep. Animal Science Papers and Reports 27(2), 127-137.
• LIU G.Y., XIONG Y.Z., 2009–Molecular cloning, polymorphism and association analyses of a novel porcine mRNA differentially expressed in the Longissimus muscle tissues from Meishan and Large White pigs. Molecular Biology Reports 36(6), 1393-1398.
• LIU G.Y., XIONG Y.Z., DENG C.Y., ZUO B., ZHANG J.H., 2004 – Comparison of gene expression patterns in longissimus dorsi of pig between the high-parent heterosis cross combination Landrace x Large White and mid-parent heterosis cross combination Large White x Meishan. Asian-Australian Journal of Animal Science 17(9), 1192-1196.
• PAN P.W., ZHAO S.H., YU M., XIONG T.A., LI K., 2003 – Identification of differentially Expressem genes in the longissimus dorsi tissue between Duroc and Erhualian pig by mRNA differential display.Asian-Australian Journal of Animal Science 16 (7), 1066-1070.
• Tomsig J.L., Sohma H., Creutz C.E., 2004 – Calcium-dependent regulation of tumour necrosis factor-alpha receptor signalling by copine. Biochemical Journal 378(Pt 3), 1089-1094.
• YAMAZAKI M, SAITO K., 2002–Differential display analysis of gene expression in plants.Cellular and Molecular Life Sciences 59(8), 1246-1255.
• Yan D.W., Bi B.L., Liu Y.G., 2010 – A novel pig gene, FASTK, differentially expressed in the muscle tissues from Wujin and Large White pigs. Animal Science Papers and Reports 28 (4), 363-371.
• Yang W., Ng P., Zhao M., Wong T.K., Yiu S.M., Lau Y.L., 2008 - Promoter-sharing by different genes in human genome--CPNE1 and RBM12 gene pair as an example. BMC Genomics 9, 456.
• Zhang Y., Gao Q., Duan S., He Y., Sun X., Jiang R., Duan Y., Zhong X., Ge J., 2008 - Upregulation of Copine1 in trabecular meshwork cells of POAG patients: a membrane proteomics approach. Molecular Vision 30(14), 1028-1036

Uwagi

PL

Rekord w opracowaniu