Increasing leaf glutathione through stem feeding does not acclimate Eucalyptus camaldulensis seedlings towards high-light stress

• Autorzy: Dempsey R.W. , Merchant A. , Tausz M.
• Języki publikacji: EN

Abstrakty

EN

Elevated foliar concentrations of glutathione (GSH) are a common stress response and potentially crucial in conferring increased stress tolerance. The present study addressed the following questions: can increased foliar GSH levels be achieved in the short term by applying a stem feeding technique to tree seedlings? If yes, will elevated GSH concentrations provide improved tolerance to the adverse effects of high-light stress? To this end Eucalyptus camaldulensis seedlings were stem fed a 5 mM GSH solution for 6–7 h before subjecting them to highlight exposure designed to induce photoinhibition. GSH in leaves was measured using a standard photometric method, and the effect of the high-light treatment was evaluated by the decrease in the optimum quantum efficiency of photosystem II (PSII) measured by chlorophyll fluorescence (Fv/Fm). Stem feeding GSH significantly increased GSH concentrations in the leaves up to 40% above control plants. Exposure to artificial high-light intensity for 3 h induced significant photoinhibition in leaves, measured by a 15% decrease in Fv/Fm. At the same time, photosynthesis and stomatal conductance measurements indicated that leaf physiology was not disrupted as a result of the stem feeding technique. However, we have no indication that elevated GSH increased tolerance; neither did it increase sensitivity of plants to high light-induced photoinhibition. This result was accompanied by maintained rates of photochemistry before and after light stress. Unlike previous GSH-related experiments increased tolerance by increasing the rate of photochemistry was not achieved in the present experiment.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 1
• Opis fizyczny: p.221-225,fig.,ref.

Twórcy

autor

Dempsey R.W.

• Department of Forest and Ecosystem Science, Melbourne School of Land and Environment, University of Melbourne, Creswick, VIC 3363, Australia

autor

Merchant A.

• Faculty of Agriculture, Food and Natural Resources, University of Sydney, Sydney, NSW 2006, Australia

autor

Tausz M.

• Department of Forest and Ecosystem Science, Melbourne School of Land and Environment, University of Melbourne, Creswick, VIC 3363, Australia

Bibliografia

• Alscher RG (1989) Biosynthesis and antioxidant function of glutathione in plants. Physiol Plant 77:457–464
• Creissen G, Firmin J, Fryer M, Kular B, Leyland N, Reynolds H, Pastori G, Wellburn A, Mullineaux PM (1999) Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress. Plant Cell 11:1277–1291
• De Kok LJ, Tausz M (2001) The role of glutathione in plant reactions and adaptation to air pollutants. In: Grill D, Tausz M, De Kok LJ (eds) Significance of glutathione in the plant adaptation to the environment. Kluwer, Dordrecht, pp 27–56
• Elstner EF, Osswald W (1994) Mechanisms of oxygen activation during plant stress. Proc Roy Soc Edinb 102B:131–154
• Foyer CH, Noctor G (2001) The molecular biology and metabolism of glutathione. In: Grill D, Tausz M, De Kok LJ (eds) Significance of glutathione in the plant adaptation to the environment. Kluwer, Dordrecht, pp 27–56
• Foyer CH, Souriau N, Perret S, Lelandais M, Kunert KJ, Pruvost C, Jouanin L (1995) Overexpression of glutathione-reductase but not glutathione synthetase leads to increases in antioxidant capacity and resistance to photoinhibition in poplar trees. Plant Physiol 109:1047–1057
• Gomez LD, Noctor G, Knight MR, Foyer CH (2004) Regulation of calcium signalling and gene expression by glutathione. J Exp Bot 55:1851–1859
• Karpinski S, Reynolds H, Karpinska B, Wingsle G, Creissen G, Mullineaux P (1999) Systemic signaling and acclimation in response to excess excitation energy in Arabidopsis. Science 284:654–657
• Kornyeyev D, Logan BA, Payton PR, Allen RD, Holaday AS (2003) Elevated chloroplastic glutathione reductase activities decrease chilling-induced photoinhibition by increasing rates of photochemistry, but not thermal energy dissipation, in transgenic cotton. Funct Plant Biol 30:101–110
• Kranner I, Grill D (1996) Determination of glutathione and glutathione disulphide in lichens: a comparison of frequently used methods. Phytochem Anal 7:24–28
• Lappartient AG, Touraine B (1997) Glutathione-mediated regulation of ATP-sulfurylase activity, SO₄²⁻ uptake, and oxidative stress response in intact canola roots. Plant Physiol 114:177–183
• Larcher L (2003) Physiological plant ecology. Springer
• Lenth RV (2006) Java applets for power and sample size. Computer software. Retrieved 11/16/2004, from http://www.stat.uiowa. edu/～rlenth/Power
• Maxwell K, Johnson GN (2000) Chlorophyll fluorescence—a practical guide. J Exp Bot 51:659–668
• Niyogi KK (1999) Photoprotection revisited: genetic and molecular approaches. Annu Rev Plant Physiol Plant Mol Biol 50:333
• Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279
• Noctor G, Gomez L, Vanacker H, Foyer CH (2002) Interactions between biosynthesis, compartmentation and transport in the control of glutathione homeostasis and signalling. J Exp Bot 53:1283–1304
• Osmond CB, Austin MP, Berry JA, Billings WD, Boyer JS, Dacey JWH, Nobel PS, Smith SD, Winner WE (1987) Stress physiology and the distribution of plants: the survival of plants in any ecosystem depends on their physiological reactions to various stresses of the environment. Bioscience 37:38–48
• Paoletti E, Manning WJ, Spaziani F, Tagliaferro F (2007) Gravitational infusion of ethylenediurea (EDU) into trunks protected adult European ash trees (Fraxinus excelsior L.) from foliar ozone injury. Environ Pollut 145:869–873
• Rennenberg H (2001) Glutathione an ancient metabolite with modern tasks. In: Grill D, Tausz M, De Kok LJ (eds) Significance of Glutathione in the plant adaptation to the environment. Kluwer, Dordrecht, pp 1–11
• Russell CA, Fillery IRP (1996) In situ ¹⁵N labeling of lupin below ground biomass. Aust J Agric Res 47:1035–1046
• Šircelj H, Tausz M, Grill G, Batič F (2005) Biochemical responses in leaves of two apple tree cultivars subjected to progressing drought. J Plant Physiol 162:1218–1308
• Štroch M, Špunda V, Kurasová I (2004) Non-radiative dissipation of absorbed excitation energy within photosynthetic apparatus of higher plants. Photosynthetica 42:323–337
• Tausz M (2001) The role of glutathione in plant response and adaptation to natural stress. In: Grill D, Tausz M, De Kok LJ (eds) Significance of glutathione in the plant adaptation to the environment. Kluwer, Dordrecht, pp 101–122
• Tausz M, Šircelj H, Grill D (2004) The glutathione system as a stress marker in plant ecophysiology: is a stress-response concept valid? J Exp Bot 55:1955–1962
• Will B, Jouanin L, Rennenberg H (2001) Protection from paraquatmediated photo-oxidative stress by glutathione in poplar (Populus tremula × P-alba) plants. Plant Biol 3:272–278
• Wingsle G, Karpinski S (1996) Differential redox regulation by glutathione of glutathione reductase and CuZn-superoxide dismutase gene expression in Pinus sylvestris L. needles. Planta 198:151–157

Identyfikatory

DOI

10.1007/s11738-010-0518-6