PL EN


Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 70 | 10 |

Tytuł artykułu

Age-related astrocytic changes in the periaqueductal gray matter (PAG) in rats

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Astrocytes are glial cells prone to morphological changes associated with age. The aim of the study was to investigate the immunoreactivity of glial fibrillary acidic protein (GFAP) in astrocytes of the periaqueductal gray matter (PAG) of the midbrain in adult and old male rats to demonstrate morphological changes associated with age and to assess morphometrically the number of astrocytes and the digital immunostaining intensity of the examined protein in PAG astrocytes of both groups of animals. In the study, 10 male Wistar rats in two age groups were used. The first group consisted of five 100-day-old animals, whereas the second comprised five 3-year-old rats. After euthanasia, the midbrain, containing PAG, was collected and embedded in paraffin blocks. Immnunohistochemical peroxidase-antiperoxidase reaction was carried out on coronal tissue sections with the use of the specific primary antibody against GFAP, goat anti-mouse IgG, peroxidase-antiperoxidase complex, and diaminobenzidine chromogen. GFAP-immunopositive PAG astrocytes were observed under a light microscope and subjected to morphometric analysis to determine their number and digital immunostaining intensity for the protein examined. GFAP-immunoreactive PAG astrocytes in 100-day-old rats showed uniform distribution. Numerous processes branching into secondary ones protruded from intensely GFAP-immunostained stellate cells. In contrast, in 3-year-old rats a significantly lower number of glial cells of different morphology was observed compared to young animals. Astrocytes had fewer primary processes without secondary branches. Morphometric analysis confirmed microscopic observations. Our findings indicate that PAG astrocytes are prone to quantitative and morphological changes with age, which, in turn, can cause disorders in emotional, pain, and defensive reactions.

Wydawca

-

Rocznik

Tom

70

Numer

10

Opis fizyczny

p.589-593,fig.,ref.

Twórcy

  • Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin
autor
  • Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin
autor
  • Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin
  • Department of Animal Anatomy and Histology, University of Life Sciences in Lublin, Akademicka 12, 20-950 Lublin

Bibliografia

  • 1. Amenta F., Bronzetti E., Sabbatini M., Vega J. A.: Astrocyte changes in aging cerebral cortex and hippocampus: A quantitative immunohistochemical study. Microsc. Res. Techniq. 1998, 43, 29-33.
  • 2. Behbehani M. M.: Functional characteristics of the midbrain periaqueductal gray. Prog. Neurobiol. 1995, 46, 575-605.
  • 3. Cerbai F., Lana D., Nosi D., Petkova-Kirova P., Zecchi S., Brothers H. M., Wenk G. L., Giovannini M. G.: The neuron-astrocyte-microglia triad in normal brain ageing and in a model of neuroinflammation in the rat hippocampus. PLoS OnE 2012, 7, p.e45250.
  • 4. Derouiche A., Frotscher M.: Astroglial processes around identified glutamatergic synapses contain glutamine synthetase: evidence for transmitter degradation. Brain Res. 1991, 552, 346-350.
  • 5. Eng L. F., Ghirnikar R. S., Lee Y. L.: Glial fibrillary acidic protein: GFAPThirty-one years (1969-2000). Neurochem. Res. 2000, 25, 1439-1451.
  • 6. Finch C. E.: Neurons, glia, and plasticity in normal brain aging. Neurobiol. Aging 2003, 24, 123-127.
  • 7. Hayakawa N., Kato H., Araki T.: Age-related changes of astrocytes, oligodendrocytes and microglia in the mouse hippocampal CA1 sector. Mech. Ageing Dev. 2007, 128, 311-316.
  • 8. Hwang I. K., Choi J. H., Li H., Yoo K.-Y., Kim D. W., Lee Ch. H., Yi S. S., Seong J. K., Lee I. S., Yoon Y. S., Won M.-H.: Changes in glial fibrillary acidic protein immunoreactivity in the dentate gyrus and hippocampus proper of adult and aged dogs. J. Vet. Med. Sci. 2008, 70, 965-969.
  • 9. Jalenques I., Burette A., Albuisson E., Romand R.: Age-related changes in GFAP-immunoreactive astrocytes in the rat ventral cochlear nucleus. Hearing Res. 1997, 107, 113-124.
  • 10. Ke Z. J., Gibson G. E.: Selective response of various brain cell types during neurodegeneration induced by mild impairment of oxidative metabolism. Neurochem. Int. 2004, 45, 361-369.
  • 11. Kushner P. D., Stephenson D. T., Wright S.: Reactive astrogliosis is widespread in the subcortical white matter of amyotrophic lateral sclerosis brain. J. Neuropathol. Exp. Neurol. 1991, 50, 263-277.
  • 12. Lynch A. M., Murphy K. J., Deighan B. F., O’Reilly J.-A., Gun’ko Y. K., Cowley T. R., Gonzalez-Reyes R. E., Lynch M. A.: The impact of glial activation in the aging brain. Aging Dis. 2010, 1, 262-278.
  • 13. Middeldorp J., Hol E. M.: GFAP in health and diesease. Prog. Neurobiol. 2011, 93, 421-443.
  • 14. Morgan T. E., Rozovsky I., Goldsmith S. K., Stone D. J., Yoshida T., Finch C. E.: Increased transcription of the astrocytes gene GFAP during middle-age is attenuated by food restriction: Implications for the role of oxidative stress. Free Radic. Biol. Med. 1997, 23, 524-528.
  • 15. Nichols N. R., Day J. R., Laping N. J., Johnson S. A., Finch C. E.: GFAP mRNA increase with age in rat and human brain. Neurobiol. Aging 1993, 14, 421-429.
  • 16. Raghavendra V., Deleo J. A.: The role of astrocytes and microglia in persistent pain. Adv. Mol. Cell Biol. 2004, 31, 951-966.
  • 17. Sloane J. A., Hollander W., Rosene D. L., Moss M. B., Kemper T., Abraham C. R.: Astrocytic hypertrophy and altered GFAP degradation with age in subcortical white matter of the rhesus monkey. Brain Res. 2000, 862, 1-10.
  • 18. Sofroniew M. V., Vinters H. V.: Astrocytes: biology and pathology. Acta Neuropathol. 2010, 119, 7-35.
  • 19. Sohal R. S., Weindruch R.: Oxidative stress, caloric restriction, and aging. Science 1996, 273, 59-63.
  • 20. Sturrock R. R.: Stability of neuron and glial number in the parabigeminal nucleus of the ageing mouse. Acta Anat. 1989, 134, 322-326.
  • 21. Vianna D. M. L., Brandão M. L.: Anatomical connections of the periaqueductal gray: specific neural substrates for different kinds of fear. Braz. J. Med. Biol. Res. 2003, 36, 557-566.
  • 22. Virgili M., Monti B., Polazzi E., Angiolini G., Contestabile A.: Topography of neurochemical alterations in the CNS of aged rats. Int. J. Dev. Neurosci. 2001, 19, 109-116.
  • 23. Walz W.: Role of glial cells in the regulation of the brain ion microenvironment. Progress in Neurobiol. 1989, 33, 309-333.
  • 24. Wu Y., Zhang A.-Q., Yew D. T.: Age-related changes of various markers of astrocytes in senescence-accelerated mice hippocampus. Neurochem. Inter. 2005, 46, 565-574.

Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

bwmeta1.element.agro-c5b79bf7-bfdd-44ed-8ab1-85677af674e6
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.