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2002 | 07 | 2A |

Tytuł artykułu

Morphological and AFLP variation of Elymus repens (L.) Gould (Poaceae)

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Combined morphological and molecular techniques were used to characterize variation in Elymus repens. We studied the morphological variability of E. repens in relation to the degree of its genetic differentiation, in order to unravel the causes of conspicuous intraspecific morphological variation. Four populations of E. repens from different habitats were analyzed for 35 morphological characters, and their genetic differentiation was assessed by Amplified Fragment Length Polymorphism (AFLP). Four pairs of selective primers were used to detect a total of 279 AFLP bands, of which 104 (37.28%) were polymorphic between populations. Cluster analysis based on AFLP fingerprint data showed that individuals were arranged in population-specific groups. The analyses of variance (ANOVA and AMOVA) indicated significant morphological and genetic differentiation among populations (P<0.01). This study revealed low levels of AFLP variation, which suggests that conspicuous morphological variation of E. repens is caused by plasticity. E. repens is an evolutionarily young species, of hybrid origin, in which microevolutionary processes continue. This study showed that common analysis of genetic diversity and morphology is a powerful tool in low-level taxonomy.

Wydawca

-

Rocznik

Tom

07

Numer

2A

Opis fizyczny

p.547-558,fig.,ref.

Twórcy

  • Department of Plant Systematics, W.Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 42, 31–512 Krakow, Poland
autor
  • Department of Plant Systematics, W.Szafer Institute of Botany, Polish Academy of Sciences, Lubicz 42, 31–512 Krakow, Poland
  • The Botanical Garden – the Centre for the Conservation of Biological Diversity, Polish Academy of Sciences, Prawdziwka 2, 02–973 Warsaw, Poland

Bibliografia

  • 1. Szczepaniak, M. Morphological variability of Polish populations of Elymus repens from various habitats - preliminary report. Ecol. Questions 2 (2001) in press.
  • 2. Ellstrand, N. C. and Roose, M. L. Patterns of genotypic diversity in clonal plant species. Amer. J. Bot. 74 (1987) 123-131.
  • 3. Pappert, R. A., Hamrick, J. L. and Donovan, L. A. Genetic variation in Pueraria lobata (Fabaceae), an introduced, clonal, invasive plant of the southeastern United States. Amer. J. Bot. 87 (2000) 1240-1245.
  • 4. Xie, Z., Lu, Y. Ge, S., Hong, D. and Li, F. Clonality in wild rice (Oryza rufipogon, Poaceae) and its implications for conservation management. Amer. J. Bot. 88 (2001) 1058-1064.
  • 5. Harper, J. L. Population Biology Of Plants. Academic Press, London, 1977.
  • 6. Escaravage, N., Questiau, S., Pornon, A., Doche, B. and Taberlet, P. Clonal diversity in a Rhododendron ferrugineum L. (Ericaceae) population inferred from AFLP markers. Mol. Ecol. 7 (1998) 975-982.
  • 7. Brej, T. Population biology of Agropyron repens (L.) P. Beauv. around the copper smelter Legnica. Acta Soc. Bot. Pol. 4 (2000) 299-309.
  • 8. Brej, T. Ecological significance of assimilate distribution in Agropyron repens clones under influence of the copper smelter Legnica. Acta Soc. Bot. Pol. 2 (2001) 145-151.
  • 9. Brej, T. Heavy metal tolerance in Agropyron repens (L.) P. Beauv. populations from the Legnica copper smelter area, lower Silesia. Acta Soc. Bot. Pol. 3-4 (1998) 325-333.
  • 10. Tsvelev, N. N. Zlaki SSSR ("Grasses of the USSR"). 788 pp. Izdatel'stvo Nauka, Leningrad, 1976, (in Russian).
  • 11. Melderis, A. Taxonomic notes on the tribe Triticeae (Gramineae), with special reference to the genera Elymus L. sensu lato, and Agropyron Gaertner sensu lato. Bot. J. Linn. Soc. 76 (1978) 369-384.
  • 12. Szabó, A. T. Wheatgrass variability {Agropyron, sect. Elytrigia) in a native collection from Transyivania. II. Agropyron repens (L.) P. Beauv. Not. Bot. Hort. Agrobot. Cluj. 11. (1981) 61-68.
  • 13. Kosina, R. Comments on the variation of spike morphology in selected species of Elytrigia and Elymus (Triticeae). Acta Soc. Bot. Pol. 64 (1995) 385-392.
  • 14. Vershinin, A., Svitashev, S., Gummesson, P. O., Salomon, B., von Bothmer, R. and Bryngelsson, T. Characterization of a family of tandemly repeated DNA sequences in Triticeae. Theor. Appl. Genet. 89 (1994) 217-225.
  • 15. Assadi, M. and Runemark, H. Hybridization, genomic constitution and generic delimitation in Elymus s.l. (Poaceae: Triticeae). Pl. Syst. Evol. 194 (1995) 189-205.
  • 16. Vos, P., Hogers, R., Bleeker, M., Reijans, M., Van de Lee, T., Hornes, M., Frijters, A., Pot, J., Peleman, J., Kuiper, M. and Zabeau, M. AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res. 23 (1995) 4407- 4414.
  • 17. Sharma, S. K., Knox, M. R. and Ellis, T. H. N. AFLP analysis of the diversity and phylogeny of Lens and its comparison with RAPD analysis. Theor. Appl. Genet. 93 (1996) 751-758.
  • 18. Qi, X. and Lindhout, P. Development of AFLP markers in barley. Mol. Gener. Gen. 254 (1997) 330-336.
  • 19. Le Thierry d’Ennequin, M., Panaud, O., Toupance, B. and Sarr, A. Assessment of genetic relationships between Setaria italica and its wild relative S. viridis using AFLP markers. Theor. Appl. Genet. 100 (2000) 1061-1066.
  • 20. Roldán-Ruiz, I., van Eeuwijk, F. A., Gilliland, T. J., Dubreuil, P., Dillmann, C., Lallemand, J., De Loose, M. and Baril, C. P. A comparative study of molecular and morphological methods of describing relationships between perennial ryegrass (Lolium perenne L.) varieties. Theor. Appl. Genet. 103 (2001) 1138-1150.
  • 21. Negi, M. S., Singh, A. and Lakshmikumaran, M. Genetic variation and relationships among and within Withania species as revealed by AFLP markers. Genome 43 (2000) 975-980.
  • 22. O’Hanlon, P. C. O., Peakall, R., Briese, D. T. A review of new PCR-based genetic markers and their utility to weed ecology. Weed Res. 40 (2000) 239-254.
  • 23. Vaz Patto, M. C., Aardse, A., Buntjer, J., Rubiales, D., Martin, A. and Niks, R. E. Morphology and AFLP markers suggest three Hordeum chilense ecotypes that differ in avoidance to rust fungi. Can. J. Bot. 79 (2001) 204- 213.
  • 24. Rabotnov, T. A. On coenopopulations of perennial herbaceous plants in natural coenoses. Vegetatio 19 (1969) 87-95.
  • 25. Sneath, P. H. and Sokal, R. R. Numerical Taxonomy. W. H. Freemen, San Francisco, 1973.
  • 26. StatSoft, Inc., 1984-1995. STATISTICA for WINDOWS, StatSoft, Inc., Tulsa.
  • 27. Bednarek, P. T., Chwedorzewska, K., Króliczak, J., Puchalski, J. and Zawada M. AFLP molecular markers as a tool for genetic variability studies of rye inbred lines. Biul. Inst. Hod. Aklim. Rośl. 211 (1999) 219-227.
  • 28. Nei, M. and Li, W. H. Mathematical model for studying genetic variation in terms of restriction endonucleases. Proc. Nat. Acad. Sci. USA 76 (1979) 5269-5269.
  • 29. Excoffier, L., Smouse, P. E. and Quattro, J. M. Analysis of molecular variance inferred from metric distances among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131 (1992) 479-491.
  • 30. Stewart, C. N. and Excoffier, L. Assessing population genetic structure and variability with RAPD data: application to Vaccinium macrocarpon (American cranberry). J. Evol. Biol. 9 (1996) 153-171.
  • 31. Schneider, S., Roessli, D. and Excoffier, L. Arlequin ver. 2.000: A software for population genetic data analysis. Genetic and Biometry Laboratory, University of Geneva, Switzerland, 2000.
  • 32. Pielou, E. C. An Introduction To Mathematical Ecology. Wiley- Interscience, New York, 1969.
  • 33. Mantel, N. A. The detection of disease clustering and a generalized regression approach. Cancer Res. 27 (1967) 209-220.
  • 34. Mizianty, M., Frey, L. and Szczepaniak, M. The Agropyron-Elymus complex (Poaceae) in Poland: biosystematics. in: Studies On Grasses In Poland, (Frey, L. Ed.) W. Szafer Institute of Botany Polish Academy of Sciences, Kraków, 2001, 25-77.
  • 35. Kardolus, J. P., van Eck, H. J. and van den Berg, R. G. The potential of AFLPs in biosystematics: a first application in Solanum taxonomy (Solanaceae). Pl. Syst. Evol. 210 (1998) 87-103.
  • 36. Hansen, K. T., Elven, R. and Brochmann, C. Molecules and morphology in concert: tests of some hypotheses in arctic Potentilla (Rosaceae). Amer. J. Bot. 87 (2000) 1466-1479.
  • 37. Steiner, E. Incompatibility studies of Oenothera: the distribution of SI alleles in biennis I populations. Evolution 18 (1964) 370-378.
  • 38. Taylor, D. R. and Aarssen, L. W. An interpretation of phenotypic plasticity in Agropyron repens (Gramineae) Amer. J. Bot. 75 (1988) 401-413.
  • 39. Schlichting, C. D. and Levin, D. A. Phenotypic plasticity: an evolving plant character. Biol. J. Linn. Soc. 29 (1986) 37-47.
  • 40. Falkowski, M. (Ed.). Trawy polskie ("Polish grasses"). Państwowe Wydawnictwo Rolnicze i Leśne, Warszawa, 1982, (in Polish).
  • 41. Scholz, H. Elytrigia arenosa (Gramineae) - ein mitteleuropäischer Relikt-Endemit. Bot. Jahrb. Syst. 115 (1993) 351-366.
  • 42. Snyder, L. A. Cytology of inter-strain hybrids and the probable origin of variability in Elymus glaucus. Amer. J. Bot. 38 (1951) 195-202.
  • 43. Russel, J. R., Fuller, J. D., Macaulay, M., Hatz, B. G., Jahoor, A., Powell, W. and Waugh, R. Direct comparison of levels of genetic variation among barley accessions detected by RFLPs, AFLPs, SSRs and RAPDs. Theor. Appl. Genet. 95 (1997) 714-722.
  • 44. Krauss, S. L. and Peakall, R. An evaluation of the AFLP fingerprinting technique for the analysis of paternity in natural populations of Persoonia mollis (Proteaceae). Aust. J. Bot. 46 (1998) 533-546.
  • 45. Sun, G. L., Salomon, B. and von Bothmer, R. Analysis of tetraploid Elymus species using wheat microsatellite markers and RAPD markers. Genome 40 (1997) 806-814.
  • 46. Sun, G. L., Diaz, O., Salomon, B. and von Bothmer, R. Genetic diversity in Elymus caninus as revealed by isozyme, RAPD, and microsatellite markers. Genome 42 (1999) 420-131.
  • 47. Guadagnuolo, R., Bianchi, D. S. and Felber, F. Specific genetic markers for wheat, spelt, and four wild relatives: comparison of isozymes, RAPDs, and wheat microsatellites. Genome 44 (2001) 610-621.
  • 48. Max, K. N., Mouchaty, S. K. and Schwaegerle, K. E. Allozyme and morphological variation in two subspecies of Dryas octopetala (Rosaceae) in Alaska. Amer. J. Bot. 86 (1999) 1637-1644.
  • 49. Fjellheim, S., Elven, R. and Brochmann, C. Molecules and morphology in concert. II. The Festuca brachyphylla complex (Poaceae) in Svalbard. Amer. J. Bot. 88 (2001) 869-882.
  • 50. Tsvelev, N. N. On some species of Koeleria Pers. and the origin of Koeleria grandis Bess. ex Gorski (Poaceae). Bot. Zhurn. 64 (1979) 985-988.
  • 51. Prokudin, Yu. N. Itogi kompleksnovo biosystematicheskovo izucheniya Elytrigia repens (Poaceae) [The results of a composite biosystematical study of Elytrigia repens (Poaceae)]. Bot. Zhurn. 67 (1982) 129-139, (in Russian).

Typ dokumentu

Bibliografia

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