Identyfication of differentially expressed genes preferably related to drought response in pigeon pea (Cajanus cajan) inoculated by arbuscular mycorrhizae fungi (AMF)

• Autorzy: Qiao G. , Wen X. , Yu L. , Ji X.
• Języki publikacji: EN

Abstrakty

EN

Pigeon pea is an ideal crop for sustainable agriculture systems in Karst areas of southwest China, which frequently suffers from the formidable water deficit. Physiologically, arbuscular mycorrhizae (AM)-colonized pigeon pea (Cajanus cajan) demonstrated a further enhanced tolerance to drought stress. To elucidate the molecular mechanism underlying the elevated tolerance, suppression subtractive hybridization (SSH) were employed to dig up the differentially expressed genes using mixed cDNAs prepared from drought-stressed and unstressed pigeon pea seedlings inoculated by AM fungi (AMF) in the present work. Both forward and reverse SSH cDNA library were constructed and a total of 768 clones were obtained. Dot-blotting expression analysis identified that 142 clones were upregulated, and 49 were downregulated during water stress. After sequencing, 182 unique expressed sequence tags (ESTs) were obtained via blast analysis, among which 142 (78%) exhibited high homology to previously identified or putative proteins, however, 40 (22%) showed no homology in the database. The upregulated (102) and downregulated (40) ESTs with significant protein homology might be sorted into 16 and 12 functional categories respectively, which involved in a broad spectrum of biological pathways. Furthermore, semi-quantitative reverse transcription (RT)-PCR was carried out for the 35 differentially expressed genes whose putative functions implicated in abiotic stress tolerances in other species, and it was verified these differentially expressed genes highly involved in drought stress tolerance of AM-colonized pigeon pea.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2012
• Tom: 34
• Numer: 5
• Opis fizyczny: p.1711-1721,fig.,ref.

Twórcy

autor

Qiao G.

• Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, Xiahui Street, Huaxi, Guiyang 550025, Guizhou, People's Republic of China

autor

Wen X.

• Guizhou Key Laboratory of Agricultural Bioengineering, Guizhou University, Xiahui Street, Huaxi, Guiyang 550025, Guizhou, People's Republic of China

autor

Yu L.

• School of Forestry Science, Guizhou University, Guiyang 550025, Guizhou, People's Republic of China

autor

Ji X.

• School of Life Science, Guizhou University, Guiyang 550025, Gizhou, People's Republic of China

Bibliografia

• Amir Hossain M, Lee Y, Cho JI, Ahn CH, Lee SK, Jeon JS, Kang H, Lee CH, An G, Park PB (2009) The bZIP transcription factor OsABF1 is an ABA responsive element binding factor that enhances abiotic stress signaling in rice. Plant Mol Biol 72:557–566
• Aslam M, Sinha VB, Singh RK, Anandhan S, Pande V, Ahmed Z (2010) Isolation of cold stress-responsive genes from Lepidium latifolium by suppressive subtraction hybridization. Acta Physiol Plant 32:205–210
• Augé RM (2001) Water relations, drought and vesicular-arbuscular mycorrhizal symbiosis. Mycorrhiza 11:3–42
• Augé RM (2004) Arbuscular mycorrhizae and soil/plant water relations. Can J Soil Sci 84:373–381
• Baisakh N, Subudhi PK, Varadwaj P (2008) Primary responses to salt stress in a halophyte, smooth cordgrass (Spartina alterniflora Loisel.). Funct Integr Genomic 8:287–300
• Bartels D, Sunkar R (2005) Drought and salt tolerance in plants. Crit Rev Plant Sci 24:23–58
• Dai YL, Qin QL, Dai DL, Kong LS, Li W, Zha XJ, Jin YJ, Tang KX (2009) Isolation and characterization of a novel cDNA encoding methyl jasmonate-responsive transcription factor TcAP2 from Taxus cuspidate. Biotechnol Lett 31:1801–1809
• Ding W, Song L, Wang X, Bi Y (2010) Effect of abscisic acid on heat stress tolerance in the calli from two ecotypes of Phragmites communis. Biol Plantarum 54:607–613
• Domoguen RL, Saxena KB, Mula MG, Sugui F, Dar WD (2010) The multiple uses of pigeonpea. http://www.sunstar.com.ph/baguio/multiple-uses-pigeon pea. Accessed 27 January 2010
• Downes CP, Gray A, Lucocq JM (2005) Probing phosphoinositide functions in signaling and membrane trafficking. Trends Cell Biol 15:259–268
• Fu XY, Zhang Z, Peng RH, Xiong AS, Liu JG, Wu LJ, Gao F, Zhu H, Guo ZK, Yao QH (2007) Isolation and characterization of a novel cDNA encoding ERF/AP2-type transcription factor OsAP25 from Oryza Sativa L. Biotechnol Lett 29:1293–1299
• Glickman MH, Ciechanover A (2002) The ubiquitin-protcasome proteolytic pathway: destruction for the sake of construction. Physiol Rev 82:373–428
• Golldack D, Lu¨king I, Yang O (2011) Plant tolerance to drought and salinity: stress regulating transcription factors and their functional significance in the cellular transcriptional network. Plant Cell Rep 30:1383–1391
• Gomathi R, Vasantha S, Thandapani V (2010) Mechanism of osmo regulation in response to salinity stress in sugarcane. Sugar Tech 12(3–4):305–311
• Gong DY, Zuo DC, Liu QG, Ban XW (2005) The application of pigeon pea in the subtropical regions of Guizhou Province. Subtrop Agric Res 1:24–26
• Gorantla M, Babu PR, Lachagari VBR, Feltus FA, Paterson AH, Reddy AR (2005) Functional genomics of drought stress response in rice: transcript mapping of annotated unigenes of an indica rice (Oryza sativa L. cv. Nagina 22). Curr Sci India 89:496–514
• Gosal SS, Wani SH, Kang MS (2009) Biotechnology and drought tolerance. J Crop Improv 23:19–54
• Guo Z, Huang MY, Lu SY, Zhao YQ, Qiao Z (2007) Differential response to paraquat induced oxidative stress in two rice cultivars on antioxidants and chlorophyll a fluorescence. Acta Physiol Plant 29:39–46
• Hsieh TH, Li CW, Su RC, Cheng CP, Sanjaya, Tsai YC, Chan MT (2010) A tomato bZIP transcription factor, SlAREB, is involved in water deficit and salt stress response. Planta 231:1459–1473
• ICRISAT (International Crop Resreach Institute for the Semi-Arid Tropics) (1998) Improvement of pigeon pea in eastern and southern Africa: Project completion report, PO Box 39063. Nairobi, Kenya
• Jin HC, Sun Y, Yang QC, Chao YH, Kang JM, Jin H, Li Y, Margaret G (2010) Screening of genes induced by salt stress from Alfalfa. Mol Biol Rep 37:745–753
• Joshi A, Dang HQ, Vaid N, Tuteja N (2010) Pea lectin receptor-like kinase promotes high salinity stress tolerance in bacteria and expresses in response to stress in planta. Glycoconjugate J 27:133–150
• Li ZH, Zhou CH, Gu Y, Zhang JY (2001) The present status of study and utilization of pigeon pea in China and its prospects. For Res 14:674–681
• Lin FY, Wang SQ, Hu YG, He BR (2008) Cloning of a s-adenosylmethionine synthetase gene from broomcorn millet (Panicum miliaceum L.) and its expression during drought and re-watering. Acta Agron Sin 34:777–782
• Liu Q, Zhu AD, Chai LJ, Zhou WJ, Yu KQ, Ding J, Xu J, Deng XX (2009) Transcriptome analysis of a spontaneous mutant in sweet orange [Citrus sinensis (L.) Osbeck] during fruit development. J Exp Bot 60:801–813
• McGonigle TP, Miller MH, Evans DG, Fairchild GL, Swan JA (1990) A new method which gives an objective measure of colonization of roots by vesicular-arbuscular mycorrhizal fungi. New Phytol 115:495–502
• Nakashima K, Ito Y, Yamaguchi-Shinozaki K (2009) Transcriptional regulatory networks in response to abiotic stresses in Arabidopsis and grasses. Plant Physiol 149:88–95
• Pandey GK, Gran JJ, Cheong YH, Kim BG, Li L, Luan S (2005) ABR₁, an APETALA₂-domain transcription factor that functions as a repressor of ABA response in Arabidopsis. Plant Physiol 139:1185–1193
• Porcel R, Aroca R, Azcón R, Ruiz-Lozano MJ (2006) PIP aquaporin gene expression in arbuscular mycorrhizal Glycine max and Lactuca sativa plants in relation to drought stress tolerance. Plant Mol Biol 60:389–404
• Qiao G, Wen XP (2010) Cloning and sequence analysis of actin gene fragment from cajanus cajan. Guizhou Agric Sci 38:5–7
• Qiao G, Wen XP, Yu LF, Ji XB (2011) The enhancement of drought tolerance for pigeon pea inoculated by arbuscular mycorrhizae fungi. Plant Soil Environ 57:541–546
• Rahaie M, Xue GP, Naghavi MR, Alizadeh H, Schenk PM (2010) A MYB gene from wheat (Triticum aestivum L.) is upregulated during salt and drought stresses and differentially regulated between salt-tolerant and sensitive genotypes. Plant Cell Rep 29:835–844
• Ramanjulu S, Bartels D (2002) Drought- and desiccation-induced modulation of gene expression in plants. Plant, Cell Environ 25:141–151
• Reddy AR, Chaitanya KV, Vivekanandan M (2004) Drought-induced responses of photosynthesis and antioxidant metabolism in higher plants. J Plant Physiol 161:1189–1202
• Riechmann JL, Meyerowitz EM (1998) The AP2/EREBP family of plant transcription factors. Biol Chem 379:633–646
• Ritchie JM, Polaszek A, Abeyasekera S, Minja E, Mviha P (2000) Pod pests and yield losses in smallholder pigeon pea in Blantyre/Shire Highlands. In: Ritchie JM (ed) Integrated crop management research in Malawi: developing technologies with farmers. Proceedings of the Final Project Workshop, Club Makokola, Mangochi, Malawi, 29 Nov–3 Dec 1999. Natural Resources Institute, Chatham UK
• Rodriguez PL (1998) Protein phosphatase 2C (PP2C) function in higher plants. Plant Mol Biol 38:919–927
• Saibo NJM, Lourenc T, Oliveira MM (2009) Transcription factors and regulation of photosynthetic and related metabolism under environmental stresses. Ann Bot-London 103:609–623
• Shinozaki K, Yamaguchi-Shinozaki K (2007) Gene networks involved in drought stress response and tolerance. J Exp Bot 58:221–227
• Shinozaki K, Yamaguchi-Shinozaki K, Seki M (2003) Regulatory network of gene expression in the drought and cold stress responses. Curr Opin Plant Biol 6:410–417
• Song YQ, Yang Q (2006) Phospholipase in plant defence signaling transduction and development of high plants. J Harbin Inst Technol 38:243–246
• Sturn A, Quackenbush J, Trajanoski Z (2002) Genesis: cluster analysis of microarray data. Bioinformatics 18:207–208
• Suzuki M, Tanaka K, Kuwano M, Yoshida KT (2007) Expression pattern of inositol phosphate-related enzymes in rice (Oryza sativa L.): implications for the phytic acid biosynthetic pathway. Gene 405:55–64
• Varshney RK, Chen WB, Li YP, Bharti AK, Saxena RK, Schlueter JA, Donoghue MTA, Azam S, Fan GY, Whaley AM, Farmer AD, Sheridan J, Iwata A, Tuteja R, Penmetsa RV, Wu W, Upadhyaya HD, Yang SP, Shah T, Saxena KB, Michael T, McCombie WR, Yang BC, Zhang GY, Yang HM, Wang J, Spillane C, Cook DR, May GD, Xu X, Jackson SA (2011) Draft genome sequence of pigeonpea (Cajanus cajan), an orphan legume crop of resource-poor farmers. Nat Biotechnol 30:83–89
• Wang HG, Zhang HL, Gao FH, Li JX, Li ZC (2007) Comparison of gene expression between upland and lowland rice cultivars under water stress using cDNA microarray. Theor Appl Genet 115:1109–1126
• Wu QS, Xia RX (2006) Arbuscular mycorrhizal fungi influence growth, osmotic adjustment and photosynthesis of citrus under well-watered and water stress conditions. J Plant Physiol 163:417–425
• Wu N, Fu K, Fu YJ, Zu YG, Chang FR, Chen YH, Liu XL, Kong Y, Liu W, Gu CB (2009) Antioxidant activities of extracts and main components of pigeon pea [Cajanus cajan (L.) Millsp.] leaves. Molecules 14:1032–1043
• Xiao BZ, Huang YM, Tang N, Xiong LZ (2007) Over-expression of a LEA gene in rice improves drought resistance under the field conditions. Theor Appl Genet 115:35–46
• Xiong LM, Schumaker KS, Zhu JK (2002) Cell signal during cold, drought, and salt stress. Plant Cell 14:S165–S183
• Zheng J, Zhao JF, Tao YZ, Wang JH, Liu YJ, Fu JJ, Jin Y, Gao P, Zhang JP, Bai YF, Wang GY (2004) Isolation and analysis of water stress-induced genes in maize seedlings by subtractive PCR and cDNA macroarray. Plant Mol Biol 55:807–823

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