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2011 | 55 | 4 |

Tytuł artykułu

Resistance to quinolones and tetracycline and its molecular background among Campylobacter strains isolated in Poland

Autorzy

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
The aim of this study was to investigate the resistance to quinolones and tetracycline of 200 C. jejuni and C. coli strains isolated from poultry and poultry meat. Among the strains tested, a high-level of resistance, ranging from 78% to 82% for nalidixid acid and ciprofloxacin, as well as from 44%-56% for tetracycline was observed. Moreover, all Campylobacter strains were examined for the molecular background of the appearing resistance. Almost all resistant strains tested by МАМА-PCR carried the Thr-86-to-Ile mutation in the gyrA gene. This mutation was not found in the sensitive strains. These results were confirmed by DNA sequencing performed on a group of selected strains. Moreover, all tetracycline-resistant, but none of the sensitive Campylobacter strains possessed the tet(O) gene. These data revealed that the antibiotic resistance of Campylobacter strains represent a considerable risk for the consumption of poultry meat and should be monitored in the poultry production chain.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

55

Numer

4

Opis fizyczny

p.613-618,fig.,ref.

Twórcy

autor
  • Department of Hygiene of Food of Animal Origin, National Veterinary Research Institute, 24-100 Pulawy, Poland

Bibliografia

  • 1. Acke E., McGill K., Quinn T., Jones B.R., Fanning S., Whyte P.: Antimicrobial resistance profiles and mechanisms of resistance in Campylobacter jejuni isolates from pets. Foodborne Pathog Dis 2009, 6, 705-710.
  • 2. Bardoň J., Kolář M., Cekanova L., Hejnar P., Koukalova D.: Prevalence of Campylobacter jejuni and its resistance to antibiotics in poultry in the Czech Republic. Zoonoses Public Health 2009, 56, 111-116.
  • 3. Bardoň J., Kolář M., Karpíšková R., Hricová K.: Prevalence of thermotolerant Campylobacter spp. in broilers at retail in the Czech Republic and their antibiotic resistance. Food Control 2011, 22, 328-332.
  • 4. Chen X., Naren G.W., Wu C.M., Wang Y., Dai L., Xia L.N., Luo P.J., Zhang Q., Shen J.Z.: Prevalence and antimicrobial resistance of Campylobacter isolates in broilers from China. Vet Microbiol 2010, 144, 133-139.
  • 5. Commission Decision 2007/516/EC of 19 July concerning a financial contribution from the Community towards a survey on the prevalence and antimicrobial resistance of Campylobacter spp. in broiler flocks and on the prevalence of Campylobacter spp. and Salmonella spp. in broiler carcasses to be carried out in the Member States. Official J Eur Union Legislation 50, 25-37.
  • 6. Connell S.R., Trieber C.A., Dinos G.P., Einfeldt E., Taylor D.E., Nierhaus K.H.: Mechanism of Tet(O)-mediated tetracycline resistance. EMBO J 2003, 22, 945-953.
  • 7. Ekdahl K., Andersson Y.: Regional risks and seasonality in travel-associated campylobacteriosis. BMC Infect Dis 2004, 29, 54.
  • 8. European Food Safety Authority (EFSA): The Community Summary Report on antimicrobial resistance in zoonotic and indicator bacteria from animals and food in the European Union in 2004-2007. EFSA J 2010.
  • 9. European Food Safety Authority (EFSA): The Community Summary Report on antimicrobial resistance in zoonotic and indicator bacteria from animals and food in the European Union in 2008. EFSA J 2010.
  • 10. Gibreel A., Tracz D.M., Nonaka L., Ngo T.M., Connell S.R., Taylor D.E.: Incidence of antibiotic resistance in Campylobacter jejuni isolated in Alberta, Canada, from 1999 to 2002, with special reference to tet(O)-mediated tetracycline resistance. Antimicrob Agents Chemother 2004, 48, 3442-3450.
  • 11. Guévremont E., Nadeau E., Sirois M., Quessy S.: Antimicrobial susceptibilities of thermophilic Campylobacter from humans, swine, and chicken broilers. Can J Vet Res 2006, 70, 81-86.
  • 12. Možina S.S., Kurinčič M., Klančnik A., Mavri A.: Campylobacter and its multi-resistance in the food chain. Trends Food Sci Technol 2011, 22, 91-98.
  • 13. Rozynek E., Dzierzanowska-Fangrat K., Korsak D., Konieczny P., Wardak S., Szych J., Jarosz M., Dzierzanowska D.: Comparison of antimicrobial resistance of Campylobacter jejuni and Campylobacter coli isolated from humans and chicken carcasses in Poland. J Food Prot 2008, 71, 602-607.
  • 14. Said M.M., El-Mohamady H., El-Beih F.M., Rockabrand D.M., Ismail T.F., Monteville M.R., Ahmed S.F., Klena J.D., Salama M.S.: Detection of gyrA mutation among clinical isolates of Campylobacter jejuni isolated in Egypt by МАМА-PCR. J Infect Dev Ctries 2010, 9, 546-554.
  • 15. Smith J.L., Fratamico P.M.: Fluoroquinolone resistance in campylobacter. J Food Prot. 2010, 73, 1141-1152.
  • 16. Sonnevend A., Rotimi V.O., Kolodziejek J., Usmani A., Nowotny N., Pál T.: High level of ciprofloxacin resistance and its molecular background among Campylobacter jejuni strains isolated in the United Arab Emirates. J Med Microbiol 2006, 55, 1533-1538.
  • 17. Wardak S., Szych J., Zasada A.A., Gierczynski R.: Antibiotic resistance of Campylobacter jejuni and Campylobacter coli clinical isolates from Poland. Antimicrob Agents Chemother 2007, 51, 1123-1125.
  • 18. Wieczorek K., Kania L, Osek J.: Antimicrobial resistance of Campylobacter - epidemiological aspects and public health threat, Medycyna Wet 2011, 67, 233- 239.
  • 19. Wieczorek K., Osek J.: Multiplex PCR assays for simultaneous identification of Campylobacter jejuni and Campylobacter coli. Medycyna Wet 2005, 61, 797-799.
  • 20. Wieczorek K.: Animicrobial resistance and virulence markers of Campylobacter jejuni and C. coli isolated from retail poultry meat in Poland. Bull Vet Inst Pulawy 2010, 54, 563-569.
  • 21. Wozniak A., Wieliczko A.: Tetracycline, erythromycin and gentamycin resistance of C. jejuni and C. coli isolated from poultry in Poland. Bull Vet Inst Pulawy 2011, 55, 51-54.
  • 22. Wozniak A.: Fluoroquinolones resistance of Campylobacter jejuni and Campylobacter coli isolated from poultry in 1994-1996 and 2005-2008 in Poland. Bull Vet Inst Pulawy 2011, 55, 15-20.
  • 23. Zirnstein G., Helsel L., Li Y., Swaminathan В., Besser J.: Characterization of gyrA mutations associated with fluoroquinolone resistance in Campylobacter coli by DNA sequence analysis and MAMA PCR. FEMS Microbiol Lett 2000, 190, 1-7.
  • 24. Zirnstein G., Li Y., Swaminathan В., Angulo F.: Ciprofloxacin resistance in Campylobacter jejuni isolates: detection of gyrA resistance mutations by mismatch amplification mutation assay PCR and DNA sequence analysis. J Clin Microbiol 1999, 37, 3276-3280.

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Bibliografia

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