Brucellosis in Mirpur, Azad Kashmir Pakistan: a livestock threat for neighboring zones

• Autorzy: Mubeen H. , Khan I. , Saleem M.H. , Akhtar R. , Ali S. , Saqib M. , Khan A.U. , Umar S.
• Języki publikacji: EN

Abstrakty

EN

The objective of the present study was to determine the prevalence of brucellosis in household animals of Mirpur, Azad Kashmir due to its geographic importance. A total of 360 blood samples of cattle, buffaloes, sheep and goats were initially screened through Rose Bengal Plate test (RBPT) and then positive samples were subjected to Enzyme Linked Immunosorbent Assay (ELISA) for confirmation and quantification of antibody titers. Molecular confirmation of serologically positive samples was performed by real time polymerase chain reaction (PCR). RBPT and ELISA showed a total of 8.6% and 6.87% positive samples respectively. The species wise seropositivity by RBPT was greater in cattle followed by buffaloes, goats and sheep. Similarly ELISA showed more seropositivity in cattle than buffaloes, while sheep and goats were negative for brucellosis by ELISA. RT-PCR revealed 100% samples positive for Brucella abortus by species specific PCR. This study revealed the presence of Brucella abortus in Mirpur for the first time. Since brucellosis is listed in transboundary diseases, its presence in this geographically important region could be a potential threat for neighboring countries.

Słowa kluczowe

EN

animal disease; household animal; cattle; sheep; goat; Mirpur city; Azad Kashmir; Pakistan; brucellosis; occupational risk; Brucella abortus; ELISA test; polymerase chain reaction; RBPT test zob.rose bengal plate test; rose bengal plate test

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2018
• Tom: 74
• Numer: 07
• Opis fizyczny: p.449-451,ref.

Twórcy

autor

Mubeen H.

• Department of Clinical Medicine and Surgery, Unive University of Veterinary and Animal Sciences, Lahore, Pakistan

autor

Khan I.

• Section of Epidemiology and Public Health, College of Veterinary and Animal Sciences, Jhang, Pakistan

autor

Saleem M.H.

• Department of Clinical Medicine and Surgery, Unive University of Veterinary and Animal Sciences, Lahore, Pakistan

autor

Akhtar R.

• Department of Pathology, Unive University of Veterinary and Animal Sciences, Lahore, Pakistan

autor

Ali S.

• Department of Wildlife and Ecosystem, Unive University of Veterinary and Animal Sciences, Lahore, Pakistan

autor

Saqib M.

• Department of Clinical Medicine and Surgery, University of Agriculture, Faisalabad, Pakistan

autor

Khan A.U.

• Friedrich Loeffler Institute, Jena, Germany

autor

Umar S.

• Department of Pathobiology, Pir Mehr Ali Shah Arid Agriculture University Rawalpind, Pakistan

Bibliografia

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Identyfikatory

DOI

10.21521/mw.6004