Inhibition of mGluR5 but not mGluR1 disrupts memory retrieval in the one-trial passive avoidance task in one-day old chicks

• Autorzy: Gieros K. , Sobczuk A. , Salinska E.
• Języki publikacji: EN

Abstrakty

EN

It is known, that group I metabotropic glutamate receptors (mGluRs) are involved in memory consolidation and reconsolidation. The Ca2+ signal derived from IP3 receptors (IP3R) stimulation via mGluRs activation, initiates protein synthesis that is necessary for complete memory consolidation, whereas it is suggested that during retrieval and reconsolidation of memory other mGluR1/5 triggered mechanisms may be involved. Recently mGluRs have received considerable attention as a potential drugs target for many neurological disorders, but their influence on learning and memory is still unclear. With the increasing number of people suffering memory dysfunction of different origin, unravelling of the mGluRs role in memory processes may be very important. The aim of this study was to detect differences in the role for mGluR1 and mGluR5 in memory retrieval at different times after initial training and remainder of the task. One-day old chicks were trained to avoid pecking metal bead covered with bitter-tasting substance methylanthranilate. Two experimental groups were given a reminder by presenting similar metal bead 2 or 24 h after initial training. Bilateral injections of mGluR1 antagonist LY367385 (7.5 nmol/hemisphere), mGluR5 antagonist MPEP (20 nmol/hemisphere) or IP3R antagonist 2-APB (2.5 nmol/ hemisphere) were made directly into the IMM region of chick brain. Injections were made immediately after initial training or reminder, and at 2, 3 and 24 hours later. Our results demonstrate, that in the one-trial passive avoidance task in chicks, injection of the mGluR1 and mGluR5 antagonists into chick brain region IMM shortly after training resulted in permanent amnesia. Injection of MPEP at other times (2, 3 and 24 h after initial training) resulted in transient amnesia observed 1-2 h after injection and lasting up to 4 hours. The same effect was observed for 2-APB. Blocking mGluR1 and mGluR5 immediately after reminder resulted in similar transient amnesia, same as blocking IP3R. Injections of MPEP or 2-APB at later times after reminder also resulted in transient amnesia, however the effect was weaker when reminder was given 24 h after training. mGluR1 antagonist applied later than in a short time after training or reminder had no effect on memory recall. Presented data suggest that at least in the chick model, activation of mGluR1 and mGluR5 is necessary for complete memory consolidation and reconsolidation, whereas mGluR5 are additionally involved in retrieval processes which is dependent on Ca2+ release from IP3 activated intracellular calcium stores.

• Wydawca: -
• Czasopismo: Acta Neurobiologiae Experimentalis
• Rocznik: 2011
• Tom: 71
• Numer: 1
• Opis fizyczny: p.146-147

Twórcy

autor

Gieros K.

• Mossakowski Medical Research Centre, Department of Neurochemistry, Polish Academy of Sciences, Warsaw, Poland

autor

Sobczuk A.

• Mossakowski Medical Research Centre, Department of Neurochemistry, Polish Academy of Sciences, Warsaw, Poland

autor

Salinska E.

• Mossakowski Medical Research Centre, Department of Neurochemistry, Polish Academy of Sciences, Warsaw, Poland