A saponin isolated from Agapanthus africanus differentially induces apoplastic peroxidase activity in wheat and displays fungicidal properties

• Autorzy: Cawood M.E. , Pretorius J.C. , van der Westhuizer J.H. , van Heerden F.R.
• Języki publikacji: EN

Abstrakty

EN

A spirostane with an attached trisaccharide, (25R)-5α-spirostane-2α,3β,5α-triol 3-O-(O-α-L-rhamnopyranosyl-( 1 + 2)-O-(β-D-galactopyranosyl-(1 + 3))-β-Dglucopyranoside), was isolated and identified from the aerial parts of Agapanthus africanus by activity-guided fractionation. Fungicidal properties of the crude extract, semi-purified fractions as well as the purified active saponin from A. africanus were screened in vitro against Fusarium oxysporum. At a concentration of 1 mg mL-1, the crude extract and semi-purified ethyl acetate and dichloromethane fractions showed significant antifungal activity. The purified saponin inhibited the in vitro mycelial growth of F. oxysporum completely (100 %) at a concentration of 125 lg mL-1. Furthermore, to verify previously observed induced resistance by crude extracts of A. africanus towards leaf rust, intercellular PR-protein activity was determined in wheat seedlings following foliar application of the purified saponin at 100 lg mL-1. In vitro peroxidase enzyme activity increased significantly (60 %) in wheat seedlings 48 h after treatment with the purified saponin, demonstrating its role as an elicitor to activate a defence reaction in wheat.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2015
• Tom: 37
• Numer: 11
• Opis fizyczny: Article: 246 [8 p.], fig.,ref.

Twórcy

autor

Cawood M.E.

• Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein, 9300, South Africa

autor

Pretorius J.C.

• Department of Plant Sciences, University of the Free State, PO Box 339, Bloemfontein, 9300, South Africa

autor

van der Westhuizer J.H.

• Directorate: Research Development, University of the Free State, PO Box 339, Bloemfontein, 9300, South Africa

autor

van Heerden F.R.

• School of Chemistry and Physics, University of KwaZulu-Natal, Private Bag X01, Scottsville, Pietermaritzburg, 3209, South Africa

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Identyfikatory

DOI

10.1007/s11738-015-1992-7