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2014 | 36 | 06 |

Tytuł artykułu

Copy number variations of functional genes influence contents of glycyrrhizic acid in Glycyrrhiza uralensis

Warianty tytułu

Języki publikacji

EN

Abstrakty

EN
Glycyrrhiza uralensis is a widely used Chinese herb and glycyrrhizic acid is believed to be its marker compound. Three key enzymes, 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), squalene synthase (SQS) and beta-amyrin synthase (β-AS), are involved in the glycyrrhizic acid biosynthetic pathway. In this paper, the relationship between copy number variations (CNVs) of HMGR, SQS and β-AS genes and the content levels of glycyrrhizic acid in G. uralensis were investigated. CNVs of the 62 G. uralensissamples from different origins were determined by real-time PCR and their glycyrrhizic acid contents were analyzed by HPLC. The real-time PCR results showed that the copy numbers of HMGR, SQS1 and β-AS in the 62 G. uralensis samples were either one copy or two copies. According to the copy number patterns of HMGR, SQS1 and β-AS, the 62 G. uralensis samples can be divided into six groups. Among the six groups, group B with two copies of HMGR, one copy of SQS1 and β-AS contained relatively higher contents of glycyrrhizic acid. The accumulation of glycyrrhizic acid was lower in the group C with two copies of β-AS, one copy of SQS1 and HMGR. The results of this work may provide a basis for enhancing the accumulation of glycyrrhizic acid in cultivars of G. uralensis.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

36

Numer

06

Opis fizyczny

p.1433-1440,fig.,ref.

Twórcy

autor
  • School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China
autor
  • School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China
autor
  • Department of Antibiotic, Beijing Institute for Drug Control, 100035 Beijing, China
autor
  • School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China
autor
  • School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China
autor
  • School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China
autor
  • School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China
autor
  • School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China

Bibliografia

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Typ dokumentu

Bibliografia

Identyfikatory

Identyfikator YADDA

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