Copy number variations of functional genes influence contents of glycyrrhizic acid in Glycyrrhiza uralensis

• Autorzy: Liu Y. , Zhan X.-J. , Li W.-D. , Gao Y. , Wen H. , Chen H.-H. , Wang L.-Q. , Liu C.-S.
• Języki publikacji: EN

Abstrakty

EN

Glycyrrhiza uralensis is a widely used Chinese herb and glycyrrhizic acid is believed to be its marker compound. Three key enzymes, 3-hydroxy-3-methylglutaryl CoA reductase (HMGR), squalene synthase (SQS) and beta-amyrin synthase (β-AS), are involved in the glycyrrhizic acid biosynthetic pathway. In this paper, the relationship between copy number variations (CNVs) of HMGR, SQS and β-AS genes and the content levels of glycyrrhizic acid in G. uralensis were investigated. CNVs of the 62 G. uralensissamples from different origins were determined by real-time PCR and their glycyrrhizic acid contents were analyzed by HPLC. The real-time PCR results showed that the copy numbers of HMGR, SQS1 and β-AS in the 62 G. uralensis samples were either one copy or two copies. According to the copy number patterns of HMGR, SQS1 and β-AS, the 62 G. uralensis samples can be divided into six groups. Among the six groups, group B with two copies of HMGR, one copy of SQS1 and β-AS contained relatively higher contents of glycyrrhizic acid. The accumulation of glycyrrhizic acid was lower in the group C with two copies of β-AS, one copy of SQS1 and HMGR. The results of this work may provide a basis for enhancing the accumulation of glycyrrhizic acid in cultivars of G. uralensis.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2014
• Tom: 36
• Numer: 06
• Opis fizyczny: p.1433-1440,fig.,ref.

Twórcy

autor

Liu Y.

• School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China

autor

Zhan X.-J.

• School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China

autor

Li W.-D.

• Department of Antibiotic, Beijing Institute for Drug Control, 100035 Beijing, China

autor

Gao Y.

• School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China

autor

Wen H.

• School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China

autor

Chen H.-H.

• School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China

autor

Wang L.-Q.

• School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China

autor

Liu C.-S.

• School of Chinese Pharmacy, Beijing University of Chinese Medicine, 100102 Beijing, China

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Identyfikatory

DOI

10.1007/s11738-014-1521-0