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2011 | 33 | 6 |
Tytuł artykułu

Profiling the differentially expressed genes in two rice varieties during rapid grain-filling stages

Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Grain filling is an important agronomic trait, which directly affects the final yield of rice. Partially filled and empty rice grains are among the factors that limit the yield of MR219, one of the highest yielding rice varieties in Malaysia. In this study, the NSF 20 K rice oligonucleotide array, which contains 20,000 70-mer oligonucleotide probes, was used for direct comparison of the transcriptomes of MR219 and MR84 (a rice variety that has higher percentage of filled grains compared to MR219), during rapid grain-filling period at 5 and 10 days after fertilization (DAF). A total of 155 and 233 genes were differentially expressed in MR219 compared to MR84 at 5 and 10 DAF, respectively; and 9 of these expression ratios were tested using quantitative real-time RT PCR. Among the differentially expressed genes identified were those encoding hexokinase, various sugar transporters, GSDL-like lipase/ acylhydrolase, brassinosteroid-insensitive 1-associated receptor kinase 1 precursor and homeobox protein GLABRA2, which were analyzed by real-time RT PCR in this study. The differences demonstrated by these genes in their transcript levels and profiles, between the two rice varieties understudied at different stages of grain filling may contribute to the formulation of hypotheses toward the understanding of poor percentage of filled grains in MR219.
Słowa kluczowe
EN
Wydawca
-
Rocznik
Tom
33
Numer
6
Opis fizyczny
p.2259-2268,fig.,ref.
Twórcy
autor
  • Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selengor Darul Eshan, Malaysia
autor
  • Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selengor Darul Eshan, Malaysia
autor
  • Institute of Tropical Agriculture, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia
autor
  • Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selengor Darul Eshan, Malaysia
  • Department of Cell and Molecular Biology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selengor Darul Eshan, Malaysia
  • Institute of Tropical Agriculture, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia
autor
  • Institute of Tropical Agriculture, Universiti Putra Malaysia, Selangor Darul Ehsan, Malaysia
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Bibliografia
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