Metabolism of red blood cells after short-term exercise in rats

• Autorzy: Spodaryk K. , Miszta H. , Dabrowski Z. , Gawronski W.
• Języki publikacji: EN

Abstrakty

EN

Spodaryk, K., Miszta, H. Dąbrowski, Z., and Gawroński, W.: Metabolism of red blood cells after short-term exercise in rats. Acta physiol, pol., 1989, 40 (4): 381-386. The aim of this study was to determine the changes of the basic parameters of the erythrocyte system and the activity of some red blood cell (RBC) enzymes prior to and after a single physical effort leading to exhaustion. The study, was carried out on male Wistar rats subjected to running on an electric rotating drum at a speed of 25 m/min. A single exercise caused a decrease in the RBC count, haemoglobin concentration (Hb) and haematocrit (Hct) by 21.9, 16.7 and 16.1%, respectively, and an increase in the reticulocyte count (Ret) by 661.5%. The exercise triggered also changes in the activities of some erythrocytic enzymes: pyruvate kinase (PK) activity increased by 12,4%, glucose-6-phosphałe dehydrogenase (G6PD) by 37.8%, glutathione reductase (GR) by 30.8% and acetylocholin- esterase (AChE) by 248,7%, These increases in the activities of RBC enzymes can be explained by an increase in the red cells turn-over.

• Wydawca: -
• Czasopismo: Acta Physiologica Polonica
• Rocznik: 1989
• Tom: 40
• Numer: 4
• Opis fizyczny: p.381-386,fig.,ref.

Twórcy

autor

Spodaryk K.

• Department of Sport Medicine, School of Physical Education, Krakow, Poland

autor

Miszta H.

• Laboratory of Animal Haematology and Toxicology, Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Krakow, Poland

autor

Dabrowski Z.

• Laboratory of Animal Haematology and Toxicology, Department of Animal Physiology, Institute of Zoology, Jagiellonian University, Krakow, Poland

autor

Gawronski W.

• Department of Sport Medicine, School of Physical Education, Krakow, Poland

Bibliografia

• 1. Beutler, E., (1975) The Red Cell - A Mannual of Biochemical Methods. Grune and Stratton, New York.
• 2. Brodthagen, U. A., Hansen, K. N., Knudsen, J. B., Jordal, R., Kristensen, O., Pauler, P. E. (1985) Red cell 2,3-DPG, ATP, and mean cell volume in highly trained athletes. Eur. J. App. Physiol. 53. 334-338.
• 3. Dąbrowski, Z., Szyguła, Z., Miszta, H. (1981) Do changes in bone marrow pressure contribute (to the egress of cells in bone marrow. Acta Physiol. Pol. 8. 729-736.
• 4. Cohen, S. N., Ekholm, J. E., Luthra, M. G., Hanahan, D. J. (/1976) Biochemical characterization of density-separated human erythrocytes. Biochim. Biophys. Acta 419. 229-242.
• 5. Ellman, G. L., Coutney, K. D., Valentino, A., Featherstone, R. M. (1961) A new and rapid colorimetric determination of acetylcholinesterase activity. Biochem. Pharmacol. 7. 88-95.
• 6. Fornaini, G., Dacha, M., Accorsi, A., Faci, A., Piatti, E. (1981) Glucose utilization in human erythrocytes during physical exerdise. Med. Sci. Sport Exercise 13, 322-324.
• 7. Haidas, S., Labie, D., Kaplan, J. C. (1971) 2,3-diphosphoglycerate content and oxygen affinity as a function of red cell age in normal individuals. Blood 38. 463-467.
• 8. Kaplan, J. C. (1971) Metabolisme erythrocytaire et transport d’oxygene. Schweiz. Med. Wschr. 101. 1751-1755.
• 9. Klein, J. P., Porster, H. V., Stewart, R. D., Wu, A. (1980) Hemoglobin affinity for oxygen during short-term exhaustive exercise. J. App. Physiol. 48. 236-242.
• 10. Krzymowski, T., Krzymowska, H. (1963) Fizjologia układu krwiotwórczego, PWN, Warszawa, 26-36.
• 11. Lenfant, C., Torrance, J., Woodron, R., Jacobs, P., Finch, C. (1970) Role of organie phosphates in (the adaptation of man to hypoxia. Fed. Proc. 29. ,1115-1118.
• 12. Mairbaurl, H., Schobersberger, W., Hasibeder, W., Schwaberger, G., Gaesser, G., Tanaka, K. R. (1986) Regulation of red cell 2,3-DPG sand Hb-O₂ -ufflnity during acute exercise. Eur. J. Appl. Physiol. 55. 174-180.
• 13. Puchl. J.L. Runyan, W. S. (1980) Hematological variations during aerobic training of college women. Res. Q. Exerc. Sports 51. 533-541.
• 14. Refsum, H. E., Jordfald, G., Stromme, S. B. (1979) Hematological changes following prolonged heavy exercise. In Advances in Exercise Physiology, 9. Kargel, Basel, pp. 91-99.
• 15. Salvo, G., Caprari, P., Samoggia, P., Mariami, G., Salvati, A. M. (1982) Human erythrocyte separation according to age on a discontinuous „Реrсоll" density gradient. Clin. Chim. Acta 122. 293-300.
• 16. Seaman, C., Wyss, S., Piomelli, S. (1980) The decline in energetic metabolism with aging of the erythrocyte and its relationship to cell death. Amer. J. Hematol. 8. 31-42.
• 17. Spodaryk, K., Szyguła, Z., Dąbrowski, Z., Miszta, H. (1985) The activity of erythrocyte enzymes in ralts subjected to running exercises. Eur. J. Appl. Physiol. 54. 533-537.
• 18. Szyguła, Z., Spodaryk, K., Dąbrowski, Z., Miszta, H. (1985) Post-efford anemia and hemolytic phenomena during physical exercises. Biol. Sport 2. 61-73.
• 19. Wiesman, U., Tonz. O., Richterach, R., Verger, P. (1965) Die Erythrocyfeh pyruvate Kinase bei gesunden und bei nichfspharocytarer hemelytischer pyruvate Kinase. Klin. Wschr. 43. 1311-1318.