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2013 | 35 | 07 |

Tytuł artykułu

Lipids in hairy roots and non-Agrobacterium induced roots of Crambe abyssinica

Warianty tytułu

Języki publikacji

EN

Abstrakty

Hairy root cultures of Crambe abyssinica were obtained through infection of leaves with two wild-type agropine strains of Agrobacterium rhizogenes. The efficiency of transformation was about 16 %. The presence of T-DNA from A. rhizogenes in the hairy roots genome was confirmed by PCR using specific primers for rolB and rolC genes. Selected clones of hairy roots and non-Agrobacterium induced roots from sterile cultures were used for analyses of acyl-lipids. The total amount of acyl-lipids per mg of dry weight was similar in both the non-Agrobacterium induced roots and the hairy roots in good physiological condition, and ranged from 38 to 53 nmol. However, in the clones which showed symptoms of ageing, the lipid content was severely reduced. Also the lipid composition of hairy roots appears to be similar to the composition of non-transformed roots. Polar lipids were the dominant class of lipids in both types of roots (about 75 %). Furthermore, we found diacylglycerols, free fatty acids (FFA), triacylglycerols, sterol esters, and an unidentified lipid class. The dominant fatty acids in the lipids of both types of roots were a-linolenic acid, palmitic acid, and linoleic acid (over 12 % of total FA). Among the lipids of both hairy roots and non-Agrobacterium induced roots of C. abyssinica, an unidentified FA was found (over 16 % of total FAs). The present study is the first example of establishment of hairy roots cultures of C. abyssinica. It also includes the first analysis of the lipids in hairy roots and non-Agrobacterium induced roots of this species.

Słowa kluczowe

Wydawca

-

Rocznik

Tom

35

Numer

07

Opis fizyczny

p.2137-2145,fig.,ref.

Twórcy

autor
  • Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdan´sk and Medical University of Gdan´sk, Kładki 24, 80-822 Gdan´sk, Poland
autor
  • Institute of Biology and Environmental Protection, Pomeranian University in Słupsk, Arciszewskiego 22b, 76-200 Słupsk, Poland
  • Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdan´sk and Medical University of Gdan´sk, Kładki 24, 80-822 Gdan´sk, Poland
autor
  • Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdan´sk and Medical University of Gdan´sk, Kładki 24, 80-822 Gdan´sk, Poland
autor
  • Department of Biotechnology, Intercollegiate Faculty of Biotechnology, University of Gdan´sk and Medical University of Gdan´sk, Kładki 24, 80-822 Gdan´sk, Poland

Bibliografia

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Bibliografia

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