Dynamics of ginsenoside biosynthesis in suspension culture of Panax quinquefolium

• Autorzy: Kochan E. , Chmiel A.
• Języki publikacji: EN

Abstrakty

EN

Biosynthesis of six saponins (ginsenosides) in suspension culture of P. quinquefolium Z₅ was investigated. Ginsenoside content in biomass reached the highest level, nearly 30 mg g⁻¹ d.w., between 25 and 30 days of the culture. Saponins were synthesized simultaneously with cell growth but their synthesis rate was not proportional to the growth rate. During the phase of rapid biomass multiplication, after which biomass reached 90% of its maximum yield, only half examined ginsenosides was produced. The second half of the final saponins yield was produced during the slow growth phase, in which only 10% of biomass was grown. During the intensive growth phase the productivity of six saponins examined per biomass (dry weight) unit was 3.4 µg mg⁻¹ d.w. Day⁻¹, however, this parameter calculated for slow growth phase reached nearly 30 µg mg⁻¹ d.w. day⁻¹. There were differences in increase of the contents of six saponins determined in biomass, and it was the highest for saponins Re (20(S)-protopanaxatriol-6-[O-α-L-rhamnopyranosyl(1 → 2)-β-D-glucopyranoside]-20-O-β-D-glucopyranoside) and Rg₁ (20(S)-protopanaxatriol-6,20-di-O-β-D-glucoside).

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2011
• Tom: 33
• Numer: 3
• Opis fizyczny: p.911-915,fig.,ref.

Twórcy

autor

Kochan E.

• Department of Biosynthesis of Drugs, Chair of Biology and Pharmaceutical Biotechnology, Medical University, Muszynskiego 1, 90-151 Lodz, Poland

autor

Chmiel A.

• Department of Biosynthesis of Drugs, Chair of Biology and Pharmaceutical Biotechnology, Medical University, Muszynskiego 1, 90-151 Lodz, Poland

Bibliografia

• Akalezi CO, Liu S, Li QS, Yu JT, Zhong JJ (1999) Combined effects of initial sucrose concentration and inoculum size on cell growth and ginseng saponin production by suspension cultures of Panax ginseng. Process Biochem 34:639–642
• Belegortsera N, Yoon JY, Kim KH (2000) Inhibition of Helicobacter pylori hemagglutination by polysaccharide fractions from roots of Panax ginseng. Planta Med 66:217–220
• Keith I, Block MD (2003) Immune system effects of echinacea, ginseng and astralagus: a review. Integr Cancer Ther 2(3):247–267
• Khodakovsaya MV, Bulgakov VP, Chernoded GK, Zhuravlev YN (1998) The approaches for the productivity improvement of the cultured ginseng cells. In: Ginseng in Europe. Proceedings of the 1st European ginseng congress. Magburg, pp 217–220
• Kochan E, Kołodziej B, Gadomska G, Chmiel A (2008) Ginsenoside contents in Panax quinquefolium organs from field cultivation. Z Naturforsch 63c:91–95
• Lee SH, Jung BH, Kim SY, Lee EH, Chung BCh (2006) The antistress effect of ginseng total saponin and ginsenoside Rg3 and Rb1 evaluated by brain polyamine level under immobilization stress. Pharmacol Res 54:46–49
• Lian XY, Zhang Z, Stringer JL (2005) Protective effect of ginseng components in a rodent model of neurodegeneration. Ann Neurol 57(5):642–648
• Liu S, Zhong JJ (1996) Effects of potassium ion on cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax ginseng. J Biotechnol 52:121–126
• Liu S, Zhong JJ (1997) Simultaneous production of ginseng saponin and polysaccharide by suspension cultures of Panax ginseng: nitrogen effects. Enzym Microb Technol 21:518–524
• Mathur A, Shukla YN, Pal M, Ahuja PS, Uniyal GC (1994) Saponin production in callus and cell suspension cultures of Panax quinquiefolium. Phytochemistry 35(5):1221–1225
• Naval MV, Gómez-Serranillos MP, Carretero ME, Villar AM (2007) Neuroprotective effect of a ginseng (Panax ginseng) root extract on astrocytes primary culture. J Ethnopharmacol 112:262–270
• Predy Gn, Goel V, Lovlin R, Donner A, Stitt L, Basu TK (2005) Efficacy of an extract of North American ginseng containing poly-furanosyl-saccharides for preventing upper respiratory tract infections: a randomized controlled trial. Can Med Assoc J 173(9):1043–1048
• Rai D, Bhatia G, Sen T, Palit G (2003) Anti-stress effect of Ginko biloba and Panax ginseng: a comparative study. J Pharmacol Sci 93:458–464
• Zhang YH, Zhong JJ, Yu JT (1996) Enhancement of ginseng saponin production in suspension cultures of Panax notoginseng: manipulation of medium sucrose. J Biotechnol 51:49–56
• Zhong JJ, Wang DJ (1996) Improvement of cell growth and production of ginseng saponin and polysaccharide in suspension cultures of Panax notoginseng Ca²⁺ effect. J Biotechnol 46:69–72
• Zhong JJ, Wang SJ (1998) Effects of nitrogen source on the production of ginseng saponin and polysaccharide by cell cultures of Panax quinquefolium. Process Biochem 33:671–675
• Zhong JJ, Chen F, Hu WW (1999) High density cultivation of Panax notoginseng cells in stirred bioreactors for the production of ginseng biomass and ginseng saponins. Process Biochem 35:491–496

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