Partial characterization of bacteriocin produced by halotolerant Pediococcus acidilactici strain QC38 isolated from traditional Cotija cheese

• Autorzy: Morales-Estrada A.I. , Lopez-Merino A. , Gutierrez-Mendez N. , Ruiz E.A. , Contreras-Rodriguez A.
• Języki publikacji: EN

Abstrakty

EN

During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source), Staphylococcus aureus, Vibrio vulnificus, Vibrio cholerae O1 Ogawa, Vibrio cholerae NO 01and Salmonella enterica subsp. Enterica serovar Typhimurium. Bacteriocin-like substance,after heating at 121°C for 15 min it remained stable and its antimicrobial activity was observed at pH ranging from 1.0 to 10.0 butinactivated by α-chymotrypsin and proteinase K. Strain QC38 was able to grow in 1–9% NaCl concentration. The plate overlay assayshowed an approximate size of bacteriocin-like substance between 3.4 and 6.5 kDa. P.acidilactici QC38 harboured a plasmid that contains a gene for a pediocin (PA-1).

• Wydawca: -
• Czasopismo: Polish Journal of Microbiology
• Rocznik: 2016
• Tom: 65
• Numer: 3
• Opis fizyczny: p.279-285,fig.,ref.

Twórcy

autor

Morales-Estrada A.I.

• Department of Microbiology, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico

autor

Lopez-Merino A.

• Department of Microbiology, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico

autor

Gutierrez-Mendez N.

• Chemistry Faculty, Universidad Autonoma de Chihuahua, Chihuahua, Mexico

autor

Ruiz E.A.

• Department of Zoology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico

autor

Contreras-Rodriguez A.

• Department of Microbiology, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico

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Identyfikatory

DOI

10.5604/17331331.1215607