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Partial characterization of bacteriocin produced by halotolerant Pediococcus acidilactici strain QC38 isolated from traditional Cotija cheese

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During a screening of lactic acid bacteria producing bacteriocin from Cotija cheese, the strain QC38 was isolated. Based on the 16S rRNA gene nucleotide sequencing (516 pb accession no KJ210322) and phylogenetic analysis, the isolate was identified as Pediococcus acidilactici. Neutralized cell-free supernatant was tested for antimicrobial activity against 17 Gram-negative and Gram-positive pathogens. Growth inhibition was achieved against Listeria monocytogenes (supplier or indication or source), Staphylococcus aureus, Vibrio vulnificus, Vibrio cholerae O1 Ogawa, Vibrio cholerae NO 01and Salmonella enterica subsp. Enterica serovar Typhimurium. Bacteriocin-like substance,after heating at 121°C for 15 min it remained stable and its antimicrobial activity was observed at pH ranging from 1.0 to 10.0 butinactivated by α-chymotrypsin and proteinase K. Strain QC38 was able to grow in 1–9% NaCl concentration. The plate overlay assayshowed an approximate size of bacteriocin-like substance between 3.4 and 6.5 kDa. P.acidilactici QC38 harboured a plasmid that contains a gene for a pediocin (PA-1).
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  • Department of Microbiology, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico
  • Department of Microbiology, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico
  • Chemistry Faculty, Universidad Autonoma de Chihuahua, Chihuahua, Mexico
  • Department of Zoology, Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional, Mexico City, Mexico
  • Department of Microbiology, Escuela Nacional de Ciencias Biologicas, Instituto Politecnico Nacional, Mexico City, Mexico
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