Hepatic stellate cells in the liver of dogs with steroid-induced hepatopathy

• Autorzy: Sobczak-Filipiak M. , Szarek J. , Czopowicz M. , Mieczkowska J. , Lechowski R.
• Języki publikacji: EN

Abstrakty

EN

Morphological lesions in hepatic stellate cells caused by the immunosuppressive doses of dexamethasone were investigated in dogs. The archival samples of liver collected during a surgical biopsy were examined. The samples were fixed in 10% buffered formalin or Carnoy's solution and then stained with routine histochemical methods. The lesions were also investigated under electron microscope. It was demonstrated that the number of stellate cells significantly increased (P = 0.0277), yet the size of cytoplasmic lipid droplets significantly decreased (P = 0.0001). Even though steroid-induced hepatopathy is considered to be a reversible pathology, and the lesions in hepatocytes under the influence of dexamethasone occur in a short period, it was found that hepatic stellate cells proliferated and underwent activation. This resulted in collagen accumulation in the hepatic sinuses. The functional and morphological disturbances in the canine liver in the course of steroid-induced hepatopathy are initially subclinical, but the changes in the structure and function of hepatic stellate cells may become a cause of lesions in the wall of hepatic sinusoidal vessels, which may induce additional functional pathologies unrelated to the damage to hepatocytes.

• Wydawca: -
• Czasopismo: Bulletin of the Veterinary Institute in Puławy
• Rocznik: 2014
• Tom: 58
• Numer: 2
• Opis fizyczny: p.273-276,fig.,ref.

Twórcy

autor

Sobczak-Filipiak M.

• Department of Pathology and Veterinary Diagnostics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-776 Warsaw, Poland

autor

Szarek J.

• Department of Pathophysiology, Forensic Veterinary Medicine and Administration, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, 10-719 Olsztyn, Poland

autor

Czopowicz M.

• Laboratory of Veterinary Epidemiology and Economics, Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-776 Warsaw, Poland

autor

Mieczkowska J.

• Division of Small Animal Internal Medicine, Department of Small Animal Diseases with Clinic; Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-776 Warsaw, Poland

autor

Lechowski R.

• Division of Small Animal Internal Medicine, Department of Small Animal Diseases with Clinic; Faculty of Veterinary Medicine, Warsaw University of Life Sciences, 02-776 Warsaw, Poland

Bibliografia

• 1. Atzori L., Poli G., Perra A.: Hepatic stellate cells: a star cell in the liver. Int J Biochem Cell Biol 2009, 41, 1639-1642.
• 2. Bancroft J.D., Cook H.C.: Manual of histological techniques and their diagnostic application. Churchill Livingstone, Edinburgh, London, Madrid, Melbourne, New York, Tokyo, 2000.
• 3. Budny T., Palmers D., Stratman U., Minin E., Herbst H., Spiegel H.U.: Morphologic features in the regenerating liver - a comparative intravital, light microscopical and ultrastructural analysis with focus on hepatic stellate cells. Virchows Arch 2007, 451, 781-791.
• 4. Carpino G., Franchito A., Morini S., Corradini S.G., Merli M., Gaudio E.: Activated hepatic stellate cells in liver cirrhosis. A morphologic and morphometrical study. Ital J Anat Embryol 2004, 109, 225-238.
• 5. Flisiak R.: Role of Ito cells in the liver function. Pol J Pathol 1997, 48, 139-145.
• 6. Friedman S.: Hepatic stellate cells: protean, multifunctional and enigmatic cells of the liver. Physiol Rev 2008, 88, 125-172.
• 7. Hellerbrand C.: Hepatic stellate cells the pericites in the liver. Pflugers Arch 2013, 465, 775-778.
• 8. Kmieć Z.: The role of hepatic stellate cells in the regulation of the liver functions. II. Cooperation with other cell types in the development of liver fibrosis. Advances in Cell Biol 2002, 30, 61-74.
• 9. Maxie M.: Jubb Kennedy and Palmer's Pathology of Domestic Animals, Vol. 2, Chapter 2, Liver and biliary system, Elsevier Saunders, Philadephia, 2007, pp. 301 -304.
• 10. Ostrowski K.: Histologia, PZWL, Warszawa, 1995.
• 11. Plewka K., Szuster-Ciesielska A., Kandefer-Szerszeń M.: Role of stellate cells in alcoholic liver fibrosis. Postępy Hig Med Dośw 2009, 63, 303-317.
• 12. Rothuizen J., Bunch S., Charles J., Cullen J., Desmet V., Szatmari V., Twedt D.. van den Ingli T.S.G.A.M., Van Winkle T., Washabau R.: WSAVA standards for clinical and histological diagnosis of canine and feline liver diseases, WSAVA Liver Standardization Group, Saunders Elsevier, Spain, 2006, pp. 41-59.
• 13. Tomanovic N.R., Boricic I.V., Brasanac D.C., Stojsic Z.M., Delic D.S., Brmbolic B.J.: Activated liver stellate cells in chronic viral C hepatitis: histopathological and immunohistochemical study. J Gastrointestin Liver Dis 2009, 18, 163-167.