High-frequency adventitious shoots regeneration from leaf of non-heading Chinese cabbage (Brassica campestris ssp. chinensis) cultured in vitro

• Autorzy: Memon S.A. , Hou X. , Zhu B. , Wolukau J. N.
• Języki publikacji: EN

Abstrakty

EN

The objective of the present work was selection of cultivar and suitable medium for regenerating shoots from leaf segments of non-heading Chinese cabbage. We evaluated six types of supplemented media with 2.0, 5.0 and 10.0 mg l⁻¹ 6-BA; 1.0 and 2.0 mg l⁻¹ TDZ; 0.1, 0.3, 0.5, 0.8 and 1.0 mg l⁻¹NAA; 3.0, 5.0 and 7.5 mg l⁻¹AgNO₃; 0.01 mg l⁻¹ 2–4, D and 4.0 mg l⁻¹ KT for shoot regeneration and six cultivars ‘‘Sanchidaye’’, ‘‘Liuchuandasuomian’’, ‘Qingyou 4’’, ‘‘Liangbaiye’’, ‘‘AiKang 5’’ and ‘‘Hanxiao F3’’, furthermore for root formation three types of supplemented media with 0.2, 0.3, 0.5 mg l⁻¹ NAA, and for survival rate two types of base media: turf + vermiculite + manure (1:2:0.2) and soil + vermiculite (1:2). Culturing leaf segments on MS medium supplemented with 2 mg l⁻¹ TDZ; 0.5 mg l⁻¹ NAA and 7.5 mg l⁻¹ AgNO₃ gave the highest number of shoots per leaf segment (66) while roots were best formed on the medium supplemented with 0.2 mg l⁻¹ NAA. Survival rate was highest (61.6%) in the turf: vermiculite: manure (1:2:0.2) medium. The highest percentage of responding leaf segments, number of shoots per leaf segment, rooting percentage and survival rate were observed in ‘‘Liuchuandasuomian’’. The plantlets were transferred to the soil and grown into mature plants in pots. These results could be used for preliminary selections of cultivars to transfer disease resistance (Bt) gene through agrobacterium in non-heading Chinese cabbage.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2009
• Tom: 31
• Numer: 6
• Opis fizyczny: p.1191-1196,fig.,ref.

Twórcy

autor

Memon S.A.

• State Key Loboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, 210095 Nanjing, China

autor

Hou X.

• State Key Loboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, 210095 Nanjing, China

autor

Zhu B.

• State Key Loboratory of Crop Genetics and Germplasm Enhancement, College of Horticulture, Nanjing Agriculture University, 210095 Nanjing, China

autor

Wolukau J. N.

• Egerton University, Nijoro, Kenya

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