Isolation and characterization of heavy metal - resistant bacterias capable of removing Cr(VI)

• Autorzy: Wang P. , Ma Y. , Wang C. , Zhang S. , Cheng S.
• Języki publikacji: EN

Abstrakty

EN

A total of 53 strains of chromium-resistant bacteria were isolated from Meiliang Bay of Taihu Lake, China and were tested for Cr(VI) resistance. The strain THKCS311 with the maximum growth value under Cr(VI) stress was regarded as the optimal strain for further study. The partial sequences were amplified from the strain and the BLAST query revealed that it was closely related to Bacillus sp., and it had 98% homologous to Bacillus mycoides strain 273 and Bacillus anthracis strain ATCC 14578. Batch experiments were conducted to remove Cr(VI) using THKCS311, and the effects of the initial Cr(VI) concentration, pH, and temperature condition on Cr(VI) removal efficiency were investigated. The results showed that Bacillus sp. can mediate reduction of Cr(VI)-Cr(III), and the removal efficiency decreased with the increase of initial Cr(VI) concentration. The removal efficiency of Cr(VI) was highest at pH 6.5 and 35ºC, and removal efficiencies were 59.2% and 60.7%, respectively. SEM micrographs indicated that THKCS311 cells were irregular and cracked with the appearance of wrinkles on the surface after Cr(VI) stress.

• Wydawca: -
• Czasopismo: Polish Journal of Environmental Studies
• Rocznik: 2015
• Tom: 24
• Numer: 1
• Opis fizyczny: p.339-345,fig.,ref.

Twórcy

autor

Wang P.

• Key Laboratory of Integrated Regulation and Resource, Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Xikang Road No.1, Gulou District, Nanjing 210098, China

autor

Ma Y.

• Key Laboratory of Integrated Regulation and Resource, Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Xikang Road No.1, Gulou District, Nanjing 210098, China

autor

Wang C.

• Key Laboratory of Integrated Regulation and Resource, Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Xikang Road No.1, Gulou District, Nanjing 210098, China

autor

Zhang S.

• Key Laboratory of Integrated Regulation and Resource, Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Xikang Road No.1, Gulou District, Nanjing 210098, China

autor

Cheng S.

• Key Laboratory of Integrated Regulation and Resource, Development on Shallow Lakes of Ministry of Education, College of Environment, Hohai University, Xikang Road No.1, Gulou District, Nanjing 210098, China

Bibliografia

• 1. DIELS L., GEETS J., DEJONGHE W., VAN R. S., VANBROEKHOVEN K., SZEWCZYK A., MALINA G. Heavy metal immobilization in groundwater by in situ bio-precipitation: comments and questions about efficiency and sustainability the process. Proceedings of the Annual International Conference on Soils, Sediments, Water and Energy. 11, 100, 2010.
• 2. PATTANAPIPITPAISAL P., BROWN N., MACASKIE L. Chromate reduction and 16S rRNA identification of bacteria isolated from a Cr(VI)-contaminated site. Appl. Microbiol. Biotechnol. 57, 257, 2001.
• 3. PALMER C. D., PULS R. W. Natural Attenuation of Hexavalent Chromium in Ground Water and Soils. EPA. Washington, D C.,USA. 1994.
• 4. CERVANTES C., CAMPOS-GARC A. J., DEVARS S., GUTI RREZ-CORONA F., LOZA-TAVERA H., TORRES-GUZM N. J. C., MORENO-S NCHEZ R. Interactions of chromium with microorganisms and plants. FEMS Microbiol. Rev. 25, 335, 2001.
• 5. CODD R., DILLON C. T., LEVINA A., LAY P. A. Studies on the genotoxicity of chromium: from the test tube to the cell. Coord. Chem. Rev. 216, 537, 2001.
• 6. OWLAD M., AROUA M., DAUD W., BAROUTIAN S. Removal of Hexavalent Chromium Contaminated Water and Wastewater: A Review. Water, Air, Soil Pollut. 200, 59, 2009.
• 7. HAN X., WONG Y. S., WONG M. H., TAM N. F. Y. Biosorption and bioreduction of Cr(VI) by a microalgal isolate, Chlorella miniata. J. Hazard. Mater. 146, 65, 2007.
• 8. CHAND R., NARIMURA K., KAWAKITA H., OHTO K., WATARI T., INOUE K. Grape waste as a biosorbent for removing Cr(VI) from aqueous solution. J. Hazard. Mater. 163, 245, 2009.
• 9. PARK D., LIM S-R., YUN Y-S., PARK J. M. Reliable evidences that the removal mechanism of hexavalent chromium by natural biomaterials is adsorption-coupled reduction. Chemosphere. 70, 298, 2007.
• 10. KRATOCHVIL D., PIMENTEL P., VOLESKY B. Removal of Trivalent and Hexavalent Chromium by Seaweed Biosorbent. Environ. Sci. Technol. 32, 2693, 1998.
• 11. FRANCISCO R., ALPOIM M. C., MORAIS P. V. Diversity of chromium-resistant and reducing bacteria in a chromium-contaminated activated sludge. J. Appl. Microbiol. 92, 837, 2002.
• 12. MEGHARAJ M., AVUDAINAYAGAM S., NAIDU R. Toxicity of Hexavalent Chromium and Its Reduction by Bacteria Isolated from Soil Contaminated with Tannery Waste. Curr. Microbiol. 47, 0051, 2003.
• 13. THACKER U., MADAMWAR D. Reduction of Toxic Chromium and Partial Localization of Chromium Reductase Activity in Bacterial Isolate DM1. World Journal of Microbiology and Biotechnology. 21, 891, 2005.
• 14. ELANGOVAN R., ABHIPSA S., ROHIT B., LIGY P., CHANDRARAJ K. Reduction of Cr(VI) by a Bacillus sp. Biotechnol. Lett. 28, 247, 2006.
• 15. VIAMAJALA S., SMITH W. A., SANI R K., APEL W. A., PETERSEN J. N., NEAL A. L., ROBERTO F. F., NEWBY D T., PEYTON B. M. Isolation and characterization of Cr(VI) reducing Cellulomonas spp. from subsurface soils: Implications for long-term chromate reduction. Bioresource Technol. 98, 612, 2007.
• 16. ZAHOOR A., REHMAN A. Isolation of Cr(VI) reducing bacteria from industrial effluents and their potential use in bioremediation of chromium containing wastewater. J. Environ. Sc. 21, 814, 2009.
• 17. LI M., CHENG X., GUO H. Heavy metal removal by biomineralization of urease producing bacteria isolated from soil. Int. Biodeterior. Biodegrad. 76, 81, 2013.
• 18. DAS S., MISHRA J., DAS S. K., PANDEY S., RAO D. S., CHAKRABORTY A., SUDARSHAN M., DAS N., THATOI H. Investigation on mechanism of Cr(VI) reduction and removal by Bacillus amyloliquefaciens, a novel chromate tolerant bacterium isolated from chromite mine soil. Chemosphere. 96, 112, 2014.
• 19. CHEN W. X., TAN Z. Y., GAO J. L., LI Y., WANG E. T. Rhizobium hainanense sp. nov., Isolated from Tropical Legumes. Int. J. Syst. Evol. Microbiol. 47, 4, 1997.
• 20. ADRIANO D. C. Trace Elements in Terrestrial Environments: Biogeochemistry, Bioavailability, and Risks of Metals. Springer, pp. 316-348, 2001.
• 21. KATHIRAVAN M., KARTHICK R., MUTHU N., MUTHUKUMAR K., VELAN M. Sonoassisted Microbial Reduction of Chromium. Appl. Biochem. Biotechnol. 160, 2000, 2010.
• 22. NARAYANI M., SHETTY K. V. Chromium-resistant bacteria and their environmental condition for hexavalent chromium removal: a review. Crit. Rev. Environ. Sci. Technol. 43, 15, 2013.
• 23. LI B., PAN D., ZHENG J., CHENG Y., MA X., HUANG F., LIN Z. Microscopic Investigations of the Cr(VI) Uptake Mechanism of Living Ochrobactrum anthropi. Langmuir. 24, 9630, 2008.
• 24. WU J., ZHANG H., HE P-J., YAO Q., SHAO L-M. Cr(VI) removal from aqueous solution by dried activated sludge biomass. J. Hazard. Mater. 176, 697, 2010.