In vitro selection for lead tolerance in shoot culture of Daphne species

• Autorzy: Wiszniewska A. , Hanus-Fajerska E. , Smolen S. , Muszynska E.

Warianty tytułu

PL

Selekcja in vitro pędów wawrzynka w kierunku tolerancji na ołów

• Języki publikacji: EN

Abstrakty

EN

In vitro culture may provide a suitable environment for selection of heavy-metal tolerant plantlets. Such clones of woody plants could be valuable material applicable to soil remediation. In in vitro culture conditions shoots of Daphne jasminea Sibth. & Sm. and Daphne tangutica Maxim. (Thymelaeaceae) were grown on media supplemented with 0.1, 0.5 and 1.0 mM lead nitrate. Level of lead bioaccumulation, growth parameters, content of photosynthetic pigments, and mineral status of cultured shoots were investigated. D. jasminea has grown vigorously on Pb2+-containing media, with growth tolerance index reaching 73–89%, depending on concentration applied, and the highest growth value was obtained in the presence of 1.0 mM lead nitrate. In vitro propagation of D. tangutica shoots was slightly inhibited by lead ions, however the growth tolerance index has increased up to 152% on medium with 1.0 mM Pb(NO3)2. In both studied species the highest content of accumulated lead, as well as the value of bioconcentration factor, were found in shoots grown on 0.1 mM lead nitrate. D. tangutica accumulated over two times as much lead in comparison with D. jasminea. Chlorophyll content in D. jasminea was not affected by applied lead nitrate doses, while in D. tangutica stimulation of chlorophyll, as well as carotenoid, synthesis occurred. In tested concentrations lead nitrate had no toxic effect on the level of shoot nutrition. Detected levels of essential and trace elements were still high enough to maintain undisturbed growth and development of cultured shoots. This is first report confirming the suitability of in vitro selection for obtaining of vigorous, proliferative, tolerant to elevated lead concentration shoots of ornamental Daphne species.

PL

W kulturze in vitro można stworzyć odpowiednie środowisko do selekcji tolerancyjnych względem podwyższonych stężeń metali ciężkich roślin drzewiastych, mogących znaleźć zastosowanie w remediacji gleby. W takich warunkach mnożono pędy wawrzynków Daphne jasminea Sibth. & Sm. i Daphne tangutica Maxim. (Thymelaeaceae), na pożywkach zawierających 0,1, 0,5 lub 1,0 mM azotanu ołowiu. Oceniano poziom bioakumulacji ołowiu, parametry wzrostu, zawartość barwników fotosyntetycznych oraz stan odżywienia mineralnego pędów. Na pożywkach zawierających jony ołowiu zanotowano intensywny wzrost gatunku D. jasminea, którego pędy charakteryzowały się dużą witalnością, a współczynnik tolerancji względem ołowiu wyniósł 73–89%, największą wartość osiągając na pożywce z dodatkiem 1,0 mM azotanu ołowiu. Obecność jonów ołowiu w pożywce ograniczyła namnażanie pędów D. tangutica, jednakże współczynnik tolerancji na pożywce zawierającej 1,0 mM Pb(NO3)2 osiągnął 152%. Dla obydwu badanych gatunków największy współczynnik bioakumulacji stwierdzono na pożywce z dodatkiem 0.1 mM azotanu ołowiu. Pędy D. tangutica akumulowały ponad dwukrotnie więcej ołowiu w porównaniu z pędami D. jasminea. Zawartość chlorofilu w pędach D. jasminea nie zmieniała się znacząco pod wpływem jonów ołowiu, natomiast w kulturach D. tangutica stwierdzono stymulację syntezy chlorofilu i karotenoidów na pożywkach zawierających Pb2+. Nie obserwowano toksycznego wpływu jonów ołowiu na poziom odżywienia mineralnego pędów. Jest to pierwsza praca dotycząca perspektywicznych gatunków ozdobnych z rodzaju wawrzynek, przedstawiająca uzyskanie żywotnych, proliferujących kultur in vitro pędów, które wykazują tolerancję na podwyższoną zawartość jonów ołowiu w pożywce.

Słowa kluczowe

EN

in vitro culture; selection; lead tolerance; bioaccumulation; heavy metal; shoot culture; Daphne; Daphne jasminea; Daphne tangutica; mineral nutrition; Thymelaeaceae

• Wydawca: -
• Czasopismo: Acta Scientiarum Polonorum. Hortorum Cultus
• Rocznik: 2015
• Tom: 14
• Numer: 1
• Opis fizyczny: p.129-142,fig.,ref.

Twórcy

autor

Wiszniewska A.

• Department of Botany and Plant Physiology, Faculty of Horticulture, University of Agriculture in Krakow, Krakow, Poland

autor

Hanus-Fajerska E.

• Department of Botany and Plant Physiology, Faculty of Horticulture, University of Agriculture in Krakow, Krakow, Poland

autor

Smolen S.

• Department of Botany and Plant Physiology, Faculty of Horticulture, University of Agriculture in Krakow, Krakow, Poland

autor

Muszynska E.

• Department of Botany and Plant Physiology, Faculty of Horticulture, University of Agriculture in Krakow, Krakow, Poland

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