Molecular identification of Candidatus Phytoplasma spp. associated with Sophora yellow stunt in Iran

• Autorzy: Allahverdi T. , Rahimian H. , Rastgou M.
• Języki publikacji: EN

Abstrakty

EN

In the spring of 2012, sophora (Sophora alopecuroides L.) plants showing symptoms of leaf yellowing, little leaves and stunting were observed in Firooz-kuh (Tehran province), Sari (Mazandaran province) and Urmia (West Azerbaijan province) in Iran. Symptomatic plants from the three locations were subjected to nested polymerase chain reaction (PCR) to amplify 16SrRNA using primer pair P1/P7 followed by primer pair R16F2n/R16R2. Th e amplicons were purifi ed, sequenced and the nucleotide sequences were analyzed by virtual restriction fragment length polymorphism (RFLP). Th e phytoplasmas associated with the yellows disease were identifi ed as members of the 16SrIX group (Candidatus Phytoplasma phoenicium) and the 16SrXII group (Candidatus Phytoplasma solani). Th e two phytoplasmas were placed in 16SrIX-C and 16SrXII-A subgroups, respectively, in constructed phylogenetic trees. Th is is the fi rst report on sophora yellows associated with Candidatus Phytoplasma phoenicium.

Słowa kluczowe

EN

molecular identification; phytoplasma symptom; sophora plant; Sophora alapecuroides; yellow stunt virus; leaf; yellowing; Iran

• Wydawca: -
• Czasopismo: Journal of Plant Protection Research
• Rocznik: 2017
• Tom: 57
• Numer: 2
• Opis fizyczny: p.167-172,fig.,ref.

Twórcy

autor

Allahverdi T.

• Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Mazandaran, Sari, Iran

autor

Rahimian H.

• Department of Plant Protection, Sari Agricultural Sciences and Natural Resources University, Mazandaran, Sari, Iran

autor

Rastgou M.

• Department of Plant Protection, College of Agriculture, Urmia University, Urmia, Iran

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Identyfikatory

DOI

10.1515/jppr-2017-0023