Use of SCAR-PCR in diagnostics of stem base pathogens of the Rhizoctonia and Oculimacula genus

• Autorzy: Lemanczyk G

Warianty tytułu

PL

Zastosowanie SCAR-PCR w diagnostyce patogenów podstawy źdźbła zbóż z rodzajów Rhizoctonia i Oculimacula

• Języki publikacji: EN

Abstrakty

EN

The aim of the paper is to compare the efficacy of SCAR-PCR assay and conventional diagnostic technique (visual assessment, isolation on PDA medium) in the identification of fungi from the genera Rhizoctonia and Oculimacula from winter triticale, rye, and barley during the shooting stage. The usefulness of molecular diagnosis of fungal pathogens in crop plants has been demonstrated. The application of SCAR- -PCR assay allowed early detection of the following pathogens: O. yallundae, O. acuformis, R. cerealis and R. solani, in plant tissues. This method was particularly effective in detection of R. solani. The research showed the usefulness of PCR markers for early detection of fungal pathogens, even if symptoms were not visible. Using the PCR technique, especially in combination with conventional methods, substantially increases the precision and effectiveness of disease diagnostics.

PL

Porównywano zastosowanie metody SCAR- -PCR i konwencjonalnej (wizualna ocena, izolacja na pożywce PDA) w identyfikacji grzybów z rodzajów Rhizoctonia i Oculimacula na formach ozimych pszenżyta, żyta i jęczmienia, pobieranych w fazie strzelania w źdźbło. Wykazano przydatność diagnostyki molekularnej patogenów grzybowych roślin w uprawach polowych. Zastosowanie metody SCAR-PCR umożliwiło wczesne wykrycie patogenów: O. yallundae, O. acuformis, R. cerealis i R. solani w tkankach roślinnych. Detekcja molekularna okazała się szczególnie skuteczna w wykrywaniu obecności R. solani. Badania wskazują na użyteczność stosowania markerów PCR do wczesnego wykrywania patogenów grzybowych, również w przypadku braku objawów chorobowych. Wykorzystanie techniki PCR zwłaszcza w połączeniu z klasycznymi metodami znacząco podnosi precyzję i skuteczność diagnostyki chorób roślin.

• Wydawca: -
• Czasopismo: Acta Agrobotanica
• Rocznik: 2011
• Tom: 64
• Numer: 4
• Opis fizyczny: p.197-206,ref.

Twórcy

autor

Lemanczyk G

• Department of Phytopathology and Molecular Mycology, University of Technology and Life Sciences in Bydgoszcz, Kordeckiego 20, 85-225 Bydgoszcz, Poland

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