Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2007 | 29 | 2 |
Tytuł artykułu

Influence of waterlogging on some anti-oxidative enzymatic activities of two barley genotypes differing in anoxia tolerance

Treść / Zawartość
Warianty tytułu
Języki publikacji
The experiment was conducted to investigate the formation of oxidative stress and the development of anti-oxidative enzymes in two barley genotypes differing in anoxia tolerance. Waterlogging led to significant reduction in root and shoot weight, green leaf area and tillers per plant, but tolerant Xiumai 3 was much less reduced than sensitive Gerdner. Malondialdehyde (MDA) content, an indicator of membrane lipid peroxidation, significantly increased in Gerdner when the plants were subjected to waterlogging, but remained little changed in Xiumai 3. Superoxide dismutase (SOD) activity was increased with waterlogging treatment and the sensitive cultivar had higher activity than the tolerant one during the experimental duration. At early stage of waterlogging treatment, both peroxidase (POD) and catalase (CAT) activities significantly increased in Xuimai 3, while obviously decreased in Gerdner. Moreover, both cultivars showed substantial increase in both POD and CAT with the progress of waterlogging exposure. Glutathione reductase (GR) activity was increased in both tolerant- and sensitive cultivars under waterlogging. It may be assumed from the current results that SOD activity appears to be not a constraining factor limiting the scavenging of ROS, and it is the change of POD and CAT activity under waterlogging that determine the status of oxidative stress. The difference between genotypes in waterlogging tolerance could be distinguished from the changed patterns of these enzymatic activities.
Słowa kluczowe
Opis fizyczny
  • Agronomy Department, Zhejiang University, Huajiashi Campus, Hangzhou 310029, China
  • Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
  • Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
  • Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan
  • Ahmed S, Nawata E, Hosokawa M, Domae Y, Sakuratani T (2002) Alterations in photosynthesis and some antioxidant enzymatic activities of mungbean subjected to waterlogging. Plant Sci 163:117–123
  • Armstrong W (1979) Aeration in higher plants. In: Woolhouse HW (ed) Advances in botanical research, vol 7. Academic, New York, pp 225–232
  • Asada K (1999) The water–water cycle in chloroplasts: scavenging of active oxygen and dissipation of excess photons. Annu Rev Plant Physiol Plant Mol Biol 50:601–639
  • Asada K, Takahashi M (1987) Production and scavenging of active oxygen in photosynthesis. In: Kyle DJ et al (eds) Photoinhibition. Elsevier, pp 227–287
  • Beyer WF, Fridovich I (1987) Assaying for superoxide dismutase activity: some large consequence of minor changes in conditions. Annal Biochem 161:559–566
  • Bowler C, Slooten L, Vandenbranden S, Derycke R, Botterman J, Sybesma M, Van Montagu M, Inze D (1991) Manganese superoxide dismutase can reduce cellular damage mediated by oxygen radicals in transgenic plant. EMBO J 10:1723–1732
  • Broadbent P, Creissen GP, Kular B, Wellburn AR, Mullineaux P (1995) Oxidative stress responses in transgenic tobacco containing altered levels of glutathione reductase activity. Plant J 8:247–255
  • Crawford RMM (1978) Metabolic adaptation to anoxia. In: Hook DD, Crawford RMM (eds) Plant life in anaerobic environments. Ann Arbor Sci Publ, Ann Arbor, Michigan, pp 119–136
  • Creissen G, Firmin J, Fryer M, Kular B, Leyland N, Reynolds H, Pastori G, Wellburn F, Baker N, Wellburn A, Mullineaux P (1999) Elevated glutathione biosynthetic capacity in the chloroplasts of transgenic tobacco plants paradoxically causes increased oxidative stress. Plant Cell 11:1277–1292
  • Foyer C, Lelandais M, Kunert JJ (1994) Photooxidative stress in plants. Plant Physiol 92:696–717
  • Gossett DR, Millhollon EP, Lucas MC (1994) Antioxidant response to NaCl stress in salt-tolerant and salt-sensitive cultivars of cotton. Crop Sci 34:706–714
  • Hendry GAF, Thorpe PC, Merzlyak MN (1993) Stress indicators-lipid peroxidation. In: Hendry GAF, Grime JP (eds) Methods in comparative plant ecology. Chapman & Hall, London, pp 154–156
  • Hwang SY, Lin HW, Chem RH, Lo HF, Li F (1999) Reduction susceptibility to waterlogging together with highlight stress is related to increases in superoxide dismutase and catalase activities in sweet potato. Plant Growth Regul 27:167–172
  • Maehly PC, Chance M (1954) The assay of catalase and peroxidases. In: Gluck D (ed) Methods of biochemical analysis. Intersciences Publishers, New York, pp 357–424
  • Meloni DA, Oliva MO, Martinez CA, Cambraia J (2003) Photosynthesis and activity of superoxide dismutase, peroxidase and glutathione reductase in cotton under salt stress. Environ Exp Bot 49:69–76
  • Noctor G, Foyer C (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279
  • Peters JL, Castillo FJ, Heath RH (1989) Alteration of extracellular enzymes in pinto bean leaves upon exposure to air pollution, ozone and sulfur dioxide. Plant Physiol 89:159–164
  • Richter C, Schweizer M (1997) Oxidative stress in mitochondria, in: Scandalios JG (ed) Oxidative stress and the molecular biology of antioxidant defense, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, Monograph Series, vol 34. pp 169–200
  • Schaedle M, Bassham JA (1977) Chloroplast glutathione reductase. Plant Physiol 59:1011–1012
  • Scandalias JG (1993) Oxygen stress and superoxide dismutase. Plant Physiol 101:7–12
  • Sgherri CLM, Pinzio C, Navari-Izzo F (1993) Chemical changes and O₂ production in thylakoid membranes under water stress. Physiol Plant 87:211–216
  • Smirnoff J, Colombe SV (1988) Drought influences the activity if enzymes of the chloroplast hydrogen peroxide scavenging system. J Exp Bot 39:1097–1109
  • Wu FB, Zhang GP, Dominy P (2003) Four barley genotypes respond differently to cadmium: lipid peroxidation and activities of antioxidant capacity. Environ Exp Bot 50:67–77
  • Zhang J, Kirkham MB (1994) Drought-stress-induced changes in activities of superoxide dismutase, catalase, and peroxidase in wheat species. Plant Cell Physiol 35:1259–1266
  • Zhang GP, Tanakamaru K, Abe J, Morita S (2006) Genotypic difference in the response of photosynthesis and soluble sugars to waterlogging in barley, Acta Physiologiae Plantarum (submitted for publication)
Typ dokumentu
Identyfikator YADDA
JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.