Chemical compounds of extracts from Sarcodon imbricatus at optimized growth conditions

• Autorzy: Sulkowska-Ziaja K. , Szewczyk A. , Gdula-Argasinska J. , Ekiert H. , Jaskiewicz J. , Muszynska B.
• Języki publikacji: EN

Abstrakty

EN

The effect of carbon and nitrogen sources and initial pH and temperature of the medium on the mycelial growth of Sarcodon imbricatus (L.) P. Karst. in axenic liquid culture was investigated. The optimal composition of the medium was found to be: 5% fructose, 1% hydrolysate of casein, 1% yeast extract, and 0.3% KH2PO4 at pH = 6 and incubation temperature of 20°C. In this condition the maximum biomass growth was observed, yielding 10.2 g L−1 of dry weight after 3-week of growth. The medium regarded as optimal for growth of S. imbricatus mycelium was used for the production of the biomass and further chemical analysis. The quantitative and qualitative composition of phenolic acids, fatty acids, and sterols were determined using chromatographic methods. The total content of phenolic acids was 1.86 mg × 100 g−1 DW, with the largest amount of protocatechuic acid (1.27 mg × 100 g−1 DW). Nineteen fatty acids were estimated, including five unsaturated fatty acids, e.g., oleic and α-linolenic acid. The analysis of sterols composition revealed the presence of ergosterol and ergosterol peroxide (197.7 and 200.47 mg × 100 g−1 DW, respectively). These compounds were isolated and confirmed by 1H-NMR. Presented study constitutes the first report on the accumulation of substances (phenolic acids, fatty acids, and sterols) with multidirectional biological activity in the mycelial axenic culture of Sarcodon imbricatus.

• Wydawca: -
• Czasopismo: Acta Mycologica
• Rocznik: 2016
• Tom: 51
• Numer: 2
• Opis fizyczny: Article 1086 [11p.], fig.,ref.

Twórcy

autor

Sulkowska-Ziaja K.

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland

autor

Szewczyk A.

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland

autor

Gdula-Argasinska J.

• Department of Radioligands, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland

autor

Ekiert H.

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland

autor

Jaskiewicz J.

• Faculty of Health and Medical Science, Andrzej Frycz Modrzewski Krakow University, Gustawa Herlinga-Grudzinskiego 1, 30-705 Krakow, Poland

autor

Muszynska B.

• Department of Pharmaceutical Botany, Faculty of Pharmacy, Jagiellonian University Medical College, Medyczna 9, 30-688 Krakow, Poland

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Identyfikatory

DOI

10.5586/am.1086