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2007 | 29 | 2 |
Tytuł artykułu

The transformation of pea (Pisum sativum L.): applicable methods of Agrobacterium tumefaciens - mediated gene transfer

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
Three methods of transformation of pea (Pisum sativum ssp. sativum L. var. medullare) were tested. The most efficient Agrobacterium tumefaciens-mediated T-DNA transfer was obtained using embryonic segments from mature pea seeds as initial explants. The transformation procedure was based on the transfer of the T-DNA region with the reporter gene uidA and selection gene bar. The expression of b-glucuronidase (GUS) in the regenerated shoots was tested using the histochemical method and the shoots were selected on a medium containing phosphinothricin (PPT). The shoots of putative transformants were rooted and transferred to non-sterile conditions. Transient expression of the uidA gene in the tissues after co-cultivation and in the course of short-term shoot cultivation (confirmed by histochemical analysis of GUS and by RT-PCR of mRNA) was achieved; however, we have not yet succeeded in proving stable incorporation of the transgene in the analysed plants.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
29
Numer
2
Opis fizyczny
p.157-163,fig.,ref.
Twórcy
autor
  • Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
autor
  • Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
autor
  • Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
autor
  • Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
autor
  • Department of Plant Biology, Mendel University of Agriculture and Forestry in Brno, Zemedelska 1, 613 00 Brno, Czech Republic
Bibliografia
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Typ dokumentu
Bibliografia
Identyfikatory
Identyfikator YADDA
bwmeta1.element.agro-bdd8cc79-49e9-4fe8-a2a8-ac2dfed78b7f
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