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2008 | 30 | 1 |
Tytuł artykułu

Oxidative events during in vitro regeneration of sunflower

Treść / Zawartość
Warianty tytułu
Języki publikacji
EN
Abstrakty
EN
The changes in the activity of some antioxidant enzymes and endogenous H2O2 level in zygotic sunflower embryos during organogenesis and somatic embryogenesis were monitored. Pathways of regeneration were induced on media differing with sucrose concentration 87 mmol dm3 for shoot [shoot induction medium (SIM) medium] and 350 mmol dm3 [embryo induction medium (EIM) medium] for somatic embryo induction. Water potential of the explants cultured on SIM increased, while the embryos maintained on EIM showed middle water deficit stress. The pattern of superoxide dismutase (SOD) isoforms was similar in organogenic and embryogenic culture; however, the intensity of MnSOD bands was higher on SIM than on EIM. Differences in catalase activity were observed: high activity on SIM predominated, whereas on EIM it was reduced. The activity of guaiacol peroxidase in the explantsproducing shoots and somatic embryos differed at the beginning of culture, but became comparable at the time of shoot and somatic embryo formation (day 5). H2O2 content was unchanged in organogenic culture, but on EIM it increased on day 1 followed by significant decrease. The results indicate that sugar concentration per se, or via induction of different developmental pathways influences the activity of antioxidant enzymes and also H2O2 level in cultured sunflower embryos.
Słowa kluczowe
Wydawca
-
Rocznik
Tom
30
Numer
1
Opis fizyczny
p.71-79,fig.,ref.
Twórcy
autor
  • Department of Plant Cytology and Embryology Jagiellonian University, Grodzka 52, 31-044 Krakow, Poland
autor
  • Department of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland
autor
  • Department of Plant Cytology and Embryology Jagiellonian University, Grodzka 52, 31-044 Krakow, Poland
  • Department of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland
autor
  • Department of Plant Physiology, Polish Academy of Sciences, Niezapominajek 21, 30-239 Krakow, Poland
  • Institute of Biology, Pedagogical Academy, Podbrzezie 3, 31-054 Krakow, Poland
Bibliografia
  • Aebi H (1984) Catalase in vitro. Methods Enzymol 105:121–126 Alscher RG, Erturk N, Heath L (2002) Role of superoxide dismutases (SODs) in controlling oxidative stress in plants. J Exp Bot 53:1331–1341
  • Bacon MA, Thompson DS, Davies WJ (1997) Can cell wall peroxidase activity explain the leaf growth response of Lolium temulentum during growth? J Exp Bot 48:2075–2085
  • Bailly C, Audigier F, Ladonne MH, Wagner F, Coste F, Corbineau D, Come D (2001) Changes in oligosaccharide content and antioxidant enzyme activities in developing bean seeds as related to acquisition of drying tolerance and seed quality. J Exp Bot 52:701–708
  • Bailly C, Leymarie J, Rousseau S, Come D, Feutry A, Corbineau F (2003) Sunflower seed development as related to antioxidant enzyme activities. In: Nicolas G, Bradford D, Come D, Pritchard H (eds) The biology of seeds: recent research advances. CAB International, Oxon, pp 69–75
  • Beachamp C, Fridovich I (1971) Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44:276–287
  • Borsani O, Diaz P, Agius MF, Valpuesta V, Monza J (2001) Water stress generates an oxidative stress through the induction of specific Cu/Zn superoxide dismutase in Lotus corniculatus leaves. Plant Sci 161:757–763
  • Bradford MM (1976) A rapid and sensitive method for quantitation of microgram quantities of protein utilizing the principle of proteindye binding. Anal Biochem 72:248–254
  • Brennan T, Frenkel C (1977) Involvement of hydrogen peroxide in the regulation of senescence in pear. Plant Physiol 59:411–416
  • Bronner R, Jeanin G, Hahne G (1994) Early cellular events during organogenesis and somatic embryogenesis induced on immature zygotic embryos of sunflower (Helianthus annuus L.). Can J Bot 72:239–248
  • Cassells AC, Curry RF (2001) Oxidative stress and physiological, epigenetic and genetic variability in plant tissue culture: implications for micropropagators and genetic engineers. Plant Cell Tissue Org Cult 64:145–157
  • Chen J, Ziv M (2001) The effect of ancymidol on hyperhydricity, regeneration, starch and antioxidant enzymatic activities in liquid-cultured Narcissus. Plant Cell Rep 20:22–27
  • Cui K, Xing G, Liu X, Xing G, Wang Y (1999) Effect of hydrogen peroxide on somatic embryogenesis of Lycium barbarum L. Plant Sci 146:9–16
  • de Klerk GJ, Arnholdt-Schmitt B, Lieberei R, Neumann KH (1997) Regeneration of roots, shoots and embryos: physiological, biochemical and molecular aspects. Biol Plant 39:53–66
  • de Marco A, Roubelakis-Angelakis KA (1996) The complexity of enzymic control of hydrogen peroxide concentration may affect the regeneration potential of plant protoplasts. Plant Physiol 110:137–145
  • Earnshaw BA, Johnson MA (1987) Control of wild carrot somatic embryo development by antioxidants. Plant Physiol 85:273–286
  • Franck T, Kevers C, Gaspar T (1995) Protective enzymatic systems against activated oxygen species compared in normal and hyperhydric shoots of Prunus avium L. raised in vitro. Plant Growth Regul 16:253–256
  • Fu J, Huang B (2001) Involvement of antioxidants and lipid peroxidation in the adaptation of two cool-season grasses to localized drought stress. Environ Exp Bot 45:105–114
  • Gamborg OL, Miller RA, Ojima K (1968) Plant cell cultures 1. Nutrient requirements of suspension cultures of soybean root cells. Exp Cell Res 50:151–158
  • Gupta SD, Datta S (2003/2004) Antioxidant enzyme activities during in vitro morphogenesis of gladiolus and the effect of application of antioxidant on plant regeneration. Biol Plant 47:179–183
  • Hiraga S, Sasaki K, Ito H, Ohasi Y, Matsui H (2001) A large family of class III plant peroxidases. Plant Cell Physiol 42:462–468
  • Hohl M, Greiner H, Schopfer P (1995) The cryptic growth response of maize coleoptiles and its relationship to H2O2 dependent cell wall stiffening. Physiol Plant 94:491–498
  • Hsiao TC (1973) Plant responses to water stress. Annu Rev Plant Physiol 24:519–570
  • Hung SH, Yu CW, Lin CH (2005) Hydrogen peroxide functions as a stress signal in plants. Bot Bull Acad Sin 46:1–10
  • Inze´ D, van Montagu M (1995) Oxidative stress in plants. Curr Opin Biotechnol 6:153–158
  • Jeanin G, Bronner R, Hahne G (1995) Somatic embryogenesis and organogenesis induced on the immature zygotic embryo of sunflower (Helianthus annuus L.) cultivated in vitro: role of the sugar. Plant Cell Rep 15:200–204
  • Jeanin G, Charriere F, Bronner R, Hahne G (1998) Is predetermined cellular competence required for alternative embryo or shoot induction on sunflower zygotic embryos? Botanica Acta 111:280–286
  • Joersbo M, Anderson JM, Okkels FT, Rajagopal R (1989) Isoperoxidases as markers of somatic embryogenesis in carrot cell suspension cultures. Physiol Plant 76:10–16
  • Kaminaka H, Morita S, Tokumoto M, Masamara T, Tanka K (1999) Differentia gene expression of rice superoxide dismutase isoforms to oxidative and environmental stresses. Free Radic Res 31:219–225
  • Kawano T (2003) Roles of the reactive oxygen species-generating peroxidase reactions in plant defence and growth induction. Plant Cell Rep 21:829–837
  • Laemmli UK (1970) Cleavage of structural proteins during assembly of the head of bacteriophage T4. Nature 227:680–685
  • Libik M, Konieczny R, Pater B, Ślesak I, Miszalski Z (2005) Differences in the activities of some antioxidant enzymes and in H2O2 content during rhizogenesis and somatic embryogenesis in callus cultures of the ice plant. Plant Cell Rep 23:834–841
  • Lin YC, Kao CH (2005) Nickel toxicity of rice seedlings: cell wall peroxidase, lignin, and NiSO4 inhibied root growth. Crop Environ Bioinfo 2:131:136
  • Maheswaran G, Williams EG (1985) Origin and development of somatic embryoids fromed directly on immature embryos of Trifolium repens in vitro. Ann Bot 56:619–630
  • Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
  • Noctor G, Foyer CH (1997) Ascorbate and glutathione: keeping active oxygen species under control. Annu Rev Plant Physiol Plant Mol Biol 49:249–279
  • Papadakis AK, Siminis CI, Roubelakis-Angelakis KA (2001) Reduced activity of antioxidant machinery is correlated with suppression of totipotency in plant protoplasts. Plant Physiol 126:434–444
  • Price AH, Atherton NM, Hendry GAF (1989) Plants under droughtstress generate activated oxygen. Free Radic Res Commun 8:61–66
  • Scandalios JG (1990) Response of plant antioxidant defence genes. In: Scandalios JG, Wright T (eds) Genomic responses to environmental stress. Academic, New York, pp 1–41
  • Scandalios JG, Guan L, Polidoros AN (1997) Catalase in plants: gene structure, properties, regulation and expression. In: Scandalios JG (eds) Oxidative Stress and Molecular Biology of Antioxidant Defenses. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 343–406
  • Siminis CI, Kanellis AK, Roubelakis-Angelakis KA (1994) Catalase is differentially expressed in dividing and nondividing protoplasts. Plant Physiol 105:1375–1383
  • van Breusegem F, Vranová E, Dat JF, Inze D (2001) The role of active oxygen species in plant signal transduction. Plant Sci 161:405–414
  • Zhou X, Han Y, Yang W, Xi T (1992) Somatic embryogenesis and analysis of peroxidase in cultured lerruce (Laruca sativa L.) cotyledons. Ann Bot 69:97–100
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