Enhancement of antioxidant enzymes activity and expression of CAT and PAL genes in hazel (Corylus avellana L.) cells in response to low-intensity ultrasound

• Autorzy: Safari M. , Ghanati F. , Behmanesh M. , Hajnorouzi A. , Nahidian B. , Mina G.
• Języki publikacji: EN

Abstrakty

EN

Induction of antioxidant systems of hazel cells by low-energy ultrasound, the potential role of hydrogen peroxide (H2O2) as a signaling molecule in regulation of activity of stress-related enzymes, and expression of catalase (CAT) and phenylalanine ammonialyase (PAL) genes were investigated. Suspension-cultured Corylus avellana L. cells were agitated by an ultrasonic device at 29 kHz with the power of 4 mW/cm2, for 8–40 min. The activities of CAT, superoxide dismutase (SOD), and ascorbate peroxidase (APX) of treated cells increased by 4, 1.7 and 7 times of the control ones, respectively. Induction of increase in the expression of CAT gene started 24 h after the treatment with ultrasound. Significant increase also was observed in the expression of PAL gene, 6 h after exposure to ultrasound, which resulted in turn to increase of total contents of soluble phenolics, 24 h of the treatment. Exposure to ultrasound up to 20 min had no adverse effects on cell viability although it slightly increased the accumulation of H2O2. However, it is likely that this level of increased H2O2 was not deteriorative for hazel cells, but rather triggered antioxidant system and provided hazel cells a sustainable growth after ultrasound treatment.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 09
• Opis fizyczny: p.2847-2855,fig.,ref.

Twórcy

autor

Safari M.

• Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran

autor

Ghanati F.

• Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran

autor

Behmanesh M.

• Department of Genetics, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran

autor

Hajnorouzi A.

• Department of Physics, Faculty of Science, K.N.Toosi University of Technology (KNTU), POB 15418-49611, Tehran, Iran

autor

Nahidian B.

• Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran

autor

Mina G.

• Department of Plant Biology, Faculty of Biological Science, Tarbiat Modares University (TMU), POB 14115-154, Tehran, Iran

Bibliografia

• Ashraf M, Ali Q (2008) Relative membrane permeability and activities of some antioxidant enzymes as the key determinants of salt tolerance in canola (Brassica napus L). Environ Exp Bot 63:266–273
• Assis JS, Maldonado R, Munoz T, Escribano MI, Merodio C (2001) Effect of high carbon dioxide concentration on PAL activity and phenolic contents in ripening cherimoya fruit. Postharvest Biol Tec 23:33–39
• Barber BP, Hiller RA, Lofstedt R, Putterman SJ, Weninger KR (1997) Defining the unknowns of sonoluminescence. Phys Rep 28: 143–165
• Breusegem FV, Vranova E, Dat JF, Inze D (2001) The role of active oxygen species in plant signal transduction. Plant Sci 161:405–414
• Chen B, Huang J, Wang J, Huang L (2008) Ultrasound effects on the antioxidative defense systems of Porphyridium cruentum. Colloid Surf B Biointerfaces 16:88–92
• Gill SS, Tuteja N (2010) Reactive oxygen species and antioxidant machinery in abiotic stress tolerance in crop plants. Plant Physiol Biochem 48:909–930
• Hajnorouzi A, Vaezzadeh M, Ghanati F, jamnezhad H, Nahidian B (2011) Growth promotion and a decrease of oxidative stress in maize seedlings by a combination of geomagnetic and weak electromagnetic fields. J Plant Physiol 168:1123–1128
• Hano C, Addi M, Bensaddek D, Cronier S, Laine E (2006) Differential accumulation of monolignol-drived compounds in elicited flax (Linum usitatissimum) cell suspension cultures. Planta 223:975–989
• Jin H, Heller DA, Kalbacova M, Kim J, Zhang J, Boghossian AA, Maheshri N, Strano MS (2010) Detection of single-molecule H2O2 signaling from epidermal growth factor receptor using fluorescent single-walled carbon nanotubes. Nat Nanotechnol 5:302–309
• Kumar RR, Sharma SK, Gadpayle KA, Singh K, Sivaranjani R, Goswami S, Rai RD (2012) Mechanism of action of hydrogen peroxide in wheat thermotolerance–interaction between antioxidant isoenzymes, proline, and cell membrane. Afr J Biotechnol 11:14368–14379
• Lin LD, Wu JY, Ho KP, Qi SY (2001) Ultrasound-induced physiological effects and secondary metabolite (saponin) production in Panax ginseng cell cultures. Ultrasound Med Biol 27:1147–1152
• Liu Y, Takatsuki H, Yoshikoshi A, Wang B, Sakanishi A (2003a) Effects of ultrasound on the growth and vacuolar H+ -ATPase activity of Aloe arborescens callus cells. Colloid Surf B 32:105–116
• Liu Y, Yoshikoshi A, Wang B, Sakanish A (2003b) Influence of ultrasonic stimulation on the growth and proliferation of Oryza sativa Nipponbare callus cells. Colloids Surface B 27:287–293
• Milowska K, Gabryelak T (2007) Reactive oxygen species and DNA damage after ultrasound exposure. Biomol Eng 24:263–267
• Oliveira I, Sousa A, Samorais J, Ferreira I, Bento A, Estevinho L, Pereira JA (2008) Chemical composition, and antioxidant and antimicrobial activities of three hazelnut (Corylus avellana L.) cultivars. Food Chem Toxicol 46:1801–1807
• Otaggio L, Bestoso F, Armirotti A, Balbi A, Damonte G, Mazzei M, Sancandi M, Miele M (2008) Taxanes from shells and leaves of Corylus avellana. J Nat Prod 7:58–60
• Qader SW, Abdulla MA, Chua LS, Najim N, Zain MM, Hamdan S (2011) Antioxidant, total phenolic content and cytotoxicity evaluation of selected Malaysian plants. Molecules 16:3433–3443
• Rezaei A, Ghanati F, Behmanesh M, Mokhtari dizaji M (2011) Ultrasound-potentiated salicylic acid-induced physiological effects and production of taxol in hazel (Corylus avellana L.) cell culture. Ultrasound Med Biol 37:1938–1947
• Safari M, Ghanati F, Hajnoruzi A, Rezaei A, Abdolmaleki P, Mokhtari-Dizaji M (2012) Maintenance of membrane integrity and increase of taxanes production in hazel (Corylus avellana L.) cells induced by low intensity ultrasound. Biotechnol Lett 34:1137–1141
• Smith MAL, Palta JP, McCown BH (1984) The measurement of isotonicity and maintenance of osmotic balance in plant protoplast manipulations. Plant Sci. Lett 33:249–258
• Sreenivasulu N, Grimm B, Wobus U, Weschke W (2000) Differential response of antioxidant compounds to salinity stress in salttolerant and salt-sensitive seedlings of foxtail millet (Setaria italica). Physiol Plant 109:435–442
• Topaz M, Shuster V, Assia E-I, Meyerstein D, Meyerstein N, Mazor D, Gedanken A (2005) Acoustic cavitation in phaco-emulsification and the role of antioxidants. Ultrasound Med Biol 31:1123–1129
• Velikova V, Yordanov I, Edreva A (2000) Oxidative stress and some antioxidant systems in acid rain-treated been plants protective role of exogenous polyamines. Plant Sci 151:59–66
• Wang JW, Zheng LP, Wu JY, Tan RX (2006) Involvement of nitric oxide in oxidative burst, phenylalanine ammonia-lyase activation and taxol production induced by low-energy ultrasound in Taxus yunnanensis cell suspension cultures. Nitric Oxide 15:351–358
• Wang P, Du Y, Li Y, Ren D, Song CP (2010) Hydrogen peroxide–mediated activation of MAP kinase 6 modulates nitric oxide biosynthesis and signal transduction in Arabidopsis. Plant Cell 22:2981–2998
• Wu J, Ge X (2004) Oxidative burst, jasmonic acid biosynthesis, and taxol production induced by low-energy ultrasound in Taxus chinensis cell suspension cultures. Biotechnol Bioeng 85: 714–721
• Zaka R, Chanal C, Misset MT (2002) Study of external low irradiation dose effects on induction of chromosome aberrations in Pisum sativum root tip meristem. Mutat Res 517:87–99

Uwagi

rekord w opracowaniu