Immunohistochemiczna identyfikacja antygenu bakteryjnego Aeromonas hydrophila w nerkach i śledzionie karpi oraz ocena aktywności fagocytów nerek po immunizacji ryb w iniekcji dootrzewnowej i w kąpieli

• Autorzy: Sopinska A. , Szubstarska D.

Warianty tytułu

EN

Immunohistochemical identification of the bacterial antigen of Aeromonas hydrophila in the kidneys and spleen of carps and evaluation of the activity of kidney phagocytes after intraperitoneal and immersion immunization

• Języki publikacji: PL

Abstrakty

EN

The aim of this study was to evaluate the activity of kidney phagocytes after immunization of fish with inactivated bacteria of Aeromonas hydrophila and to determine the location and permanence of the bacterial antigen in the lymphoid organs, the kidney and the spleen, of carps immunized intraperitoneally (group I) and by immersion (group II) at 12°C. We examined the absorption of bacteria by kidney leucocytes (PK%), the killing capacity of phagocytes (IB) and chemiluminescent activity (CL). Evaluations were performed 12 h, as well as 3, 7, 14 and 28 days after immunization. At all these time points, the values of the parameters examined were higher in fish immunized intraperitoneally than in those immunized by immersion. The absorption of bacterial cells by kidney phagocytes was particularly high after 14 days after immunization in fish group I whereas their killing and chemiluminescent activity was particularly high in 7 days after immunization in group I. Immunohistochemical examination revealed an extensive distribution and large amounts of the antigen in the interstitial part and melanomacrophage centers of the kidneys and spleen after the intraperitoneal administration of the antigen, By contrast, small amounts of the antigen were found in these organs after it had been administered by immersion.

Słowa kluczowe

PL

ryby; karpie; immunizacja; immunizacja doodtrzewnowa; immunizacja w kapieli; lokalizacja antygenu; nerki; sledziona; Aeromonas hydrophila; antygeny bakteryjne; identyfikacja; metody immunohistochemiczne; fagocyty; aktywnosc fagocytarna

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2019
• Tom: 75
• Numer: 10
• Opis fizyczny: s.599-604,tab.,fot.,bibliogr.

Twórcy

autor

Sopinska A.

• Zakład Chorób Ryb i Biologii, Wydział Medycyny Weterynaryjnej, Uniwersytet Przyrodniczy w Lublinie, ul.Akademicka 12, 20-033 Lublin

autor

Szubstarska D.

• SLW BIOLAB Weterynaryjne Laboratorium Diagnostyczne, ul.Grunwaldzka 62, 14-100 Ostróda

Bibliografia

• Adams A., de Mateo M. M.: Immunohistochemical detection of fish pathogens, [w:] Stolen J. S., Fletcher T. C., Rowley A. F., Zelikoff J. T., Kaattari S. L., Smith S. A.: Techniques in Fish Immunology – 3. SOS Publications, Fair Haven, USA 1994, s. 133-143.
• Ainsworth A. J., Dexiang C., Waterstrat P. R., Greenway T.: Effect of temperature on the immune system of channel catfish (Ictalurus punctatus) – I. Leucocyte distribution and phagocyte function in the anterior kidney at 10°C. Comp. Biochem. Physiol. Part A. Physiol. 1991, 100, 907-912.
• Angelidis P., Baudin-Laurencin F., Youinou P.: Effects of temperature on chemiluminescence of phagocytes from sea bass, Dicentrarchus labrax L. Fish Shellfish Immunol. 1988, 11, 281-288.
• Arnesen S. M., Schrøder M. B., Dalmo R. A., Bøgwald J.: Antigen uptake and immunoglobulin production in Atlantic cod (Gadus morhua L.) after intraperitoneal injection of Vibrio anguillarum. Fish Shellfish Immunol. 2002, 13, 159-170.
• Azad I. S., Shankar K. M., Mohan C. V., Kalita B.: Uptake and processing of biofilm and free-cell vaccines of Aeromonas hydrophila in indian major carps and common carp following oral vaccination-antigen localization by a monoclonal antibody. Dis. Aquat. Organ. 2000, 43, 103-108.
• Baba T., Imamura J., Izawa K., Ikeda K.: Immune protection in carp, Cyprinus carpio L., after immunization with Aeromonas hydrophila crude lipopolysaccharide. J. Fish Dis. 1988, 11, 237-244.
• Collazos M. E., Ortega E., Barriga C.: Effect of temperature on the immune system of a cyprinid fish (Tinca tinca, L.). Blood phagocyte function at low temperature. Fish Shellfish Immunol. 1994, 4, 231-238.
• Dalmo R. A., Seljelid R.: The immunomodulatory effect of LPS, laminaran and sulphated laminaran [β(1,3)-D-glucan] on Atlantic salmon, Salmo salar L., macrophages in vitro. J. Fish Dis. 1995, 18, 175-185.
• Ellis A. E., Munroe A. L. S., Roberts R. J.: Defence mechanisms in fish. 1. A study of the phagocytic system and the fate of intraperitoneally injected particulate material in the plaice (Pleuronectes platessa L.). J. Fish Biol. 1976, 8, 67-78.
• Espenes A., Press C. McL., Reitan L. J., Landsverk T.: The trapping of intravenously injected extracellular products from Aeromonas salmonicida in head kidney and spleen of vaccinated and nonvaccinated Atlantic salmon, Salmo salar L. Fish Shellfish Immunol. 1996, 6, 413-426.
• Grochoła A., Sopińska A., Puk K.: Wpływ immunostymulacji karpi LPS Aeromonas hydrophila na odporność nieswoistą i wrażliwość na zakażenie w teście challenge. Med. Weter. 2015, 71, 176-181.
• Gudding R., Lillehaug A., Evensen Ø.: Recent developments in fish vaccinology. Vet. Immunol. Immunopathol. 1999, 72, 203-212.
• Jansson E., Hongslo T., Lindberg R., Ljungberg O., Svensson B. M.: Detection of Renibacterium salmoninarum and Yersinia ruckeri by the peroxidase-antiperoxidase immunohistochemical technique in melanin-containing cells of fish tissues. J. Fish Dis. 1991, 14, 689-692.
• Kodama H., Tijiwa K., Moritomo T., Nakanishi T.: Granulocyte responses to experimental injection of live and formalin-killed bacteria in carp (Cyprinus carpio). Vet. Immunol. Immunopathol. 2002, 90, 101-105.
• Kozińska A., Antychowicz J.: Influence of an experimental Aeromonas hydrophila vaccine on selected haematological values and non-specific immunity in carp (Cyprinus carpio L.). Bull. Vet. Inst. Pulawy 2000, 44, 169-178.
• Kubala L., Lojek A., Čiž M., Vondráček J., Duškowá M., Slavíková H.: Determination of phagocyte activity in whole blood of carp (Cyprinus carpio) by luminol-enhanced chemiluminescence. Vet. Med. (Praha) 1996, 41, 323-327.
• Lamers C. H. J.: The reaction of the immune system of fish to vaccination. WAU disseration no 1029, 1985a.
• Lamers C. H. J., de Haas M. J. H.: Antigen localization in the lymphoid organs of carp (Cyprinus carpio). Cell Tissue Res. 1985b, 242, 491-498.
• Lamers C. H. J., de Haas M. J. H., Van Muiswinkel W. B.: The reaction of the immune system of fish to vaccination: development of immunological memory in carp, Cyprinus carpio L., following direct immersion in Aeromonas hydrophila bacterin. J. Fish Dis. 1985c, 8, 253-262.
• Mantur M., Wołosowicz N., Kemona H., Prokopowicz J.: Bactericidal activity of blood pelets: its determination and normal values. Acta. Med. Pol. 1986, 27, 3-4.
• Międzybrodzki J., Zemanek G., Baran J.: Chemiluminescencja – opis zjawiska, możliwości jego detekcji i wykorzystania. Mikrobiol. Med. 1999, 1, 41-46.
• Moore J. D., Ototake M., Nakanishi T.: Particulate antigen uptake during immersion immunisation of fish: The effectiveness of prolonged exposure and the roles of skin and gill. Fish Shellfish Immunol. 1998, 8, 393-408.
• Morvan C. le, Clerton P., Deschaux P., Troutaud D.: Effects of environmental temperature on macrophage activities in carp. Fish Shellfish Immunol. 1997, 7, 209-212.
• Muiswinkel W. B. van, Nakao M.: A short history of research on immunity to infectious diseases in fish. Dev. Comp. Immunol. 2014, 43, 130-150.
• O’Neil J. G.: An in vitro study of polymorphonuclear phagocytosis and the effect of temperature, [w:] Manning M. J., Tanner M. F. (eds.): Fish Immunology. New York Academic Press 1985, s. 47-55.
• Ototake M., Nakanishi T.: Kinetics of bovine serum albumin in fish plasma after hyperosmotic infiltration treatment: comparison between marine and freshwater fish. Aquaculture 1992, 103, 229-240.
• Press C. McL., Evensen Ø., Reitan L. J., Landsverk T.: Retention of furunculosis vaccine components in Atlantic salmon, Salmo salar L. following different routes of vaccine administration. J. Fish Dis. 1995, 19, 215-224.
• Roitt I., Brostoff J., Male D.: Immunologia. PZWL, Wydawnictwo medyczne Słotwiński Verlag 2000.
• Sakai M., Miyama K., Atsuta S., Kobayashi K.: The chemiluminescent responses of phagocytic cells isolated of coho salmon, rainbow trout and carp against Renibacterium salmoninarum. Fish Shellfish Immunol. 1996, 6, 71-73.
• Scott A. L., Klesius P. H.: Chemiluminescence: a novel analysis of phagocytosis in fish. Dev. Biol. Stand. 1981, 49, 243-254.
• Secombes C. J.: Enhancement of fish phagocyte activity. Fish Shellfish Immunol. 1994, 4, 421-426.
• Secombes C. J., Fletcher T. C.: The role of phagocytes in the protective mechanisms of fish. Annu. Rev. Fish Dis. 1992, 2, 53-71.
• Secombes C. J., Manning M. J., Ellis A. E.: The effect of primary and secondary immunization on the lymphoid tissues of carp, Cyprinus carpio L. J. Exp. Zool.1982, 220, 277-287.
• Sharp G. J. E., Secombes C. J.: The role of reactive oxygen species in the kiling of the bacterial fish pathogen Aeromonas salmonicida by rainbow trout macrophages. Fish Shellfish Immunol. 1993, 3, 119-129.
• Sopińska A., Szubstarska D.: Próba zastosowania metody immunohistochemicznej do wykrywania antygenu bakteryjnego ryb. Med. Weter. 2002, 1, 67-75.
• Stave J. W., Robertson B. S., Hetrick F. M.: Chemiluminescence of phagocytic cells isolated from the pronephros of striped bass. Dev. Comp. Immunol. 1983, 7, 269-276.
• Stensvåg K., Arnesen S. M., Bøgwald J., Jørgensen T. Ø.: Localization of purified surface molecules (lipopolysaccharide and A-protein) from Aeromonas salmonicida in the kidney and spleen Atlantic salmon, Salmo salar L. J. Fish Dis. 1999, 22, 337-349.
• Stevenson R. M. W.: Vaccination against Aeromonas hydrophila, [w:] Ellis A. E. (ed.): Fish vaccination. Academic Press 1988, s. 1-19.
• Tsujii T., Seno S.: Melano-macrophage centers in the aglomerular kidney of the sea horse (teleosts): morphologic studies on its formation and posssible function. Anat. Rec.1990, 226, 460-470.
• Yamada F., Kodama H., Mikami T., Izawa H.: Chemiluminescence of rainbow trout (Salmo gairdneri) phagocytes and factors affecting their response. Jpn. J. Vet. Sci. 1988, 50, 1092-1098.

Identyfikatory

DOI

10.21521/mw.6296