Effects of Schistosoma mansoni infection on lutein and beta-carotene concentrations in Biomphalaria glabrata snails as determined by quantitative high performance reversed phase thin-layer chromatography

• Autorzy: Dieterich N. , Fried B. , Sherma J.
• Języki publikacji: EN

Abstrakty

EN

High performance thin-layer chromatography was used to determine the concentration of β-carotene and lutein in the whole body and digestive gland-gonad complex (DGG) of uninfected Biomphalaria glabrata snails and those infected with Schistosoma mansoni for 6 and 8 weeks. Pigments were extracted from the snails using acetone and separated on EMD Millipore reversed phase C-18 plates with concentration zone using petroleum ether-acetonitrile-methanol (1:1:2) mobile phase. After development, two yellow pigment zones, lutein and β-carotene, were identified with respective R f values of 0.55 and 0.13 and then quantified by densitometry. Statistical analysis of the weight percentages of each pigment showed a significant decrease (P < 0.05) in the concentration of β-carotene in the DGGs of infected B. glabrata at 6 and 8 weeks post-infection compared to the uninfected snails. No significant differences were seen in the concentrations of β-carotene in the whole body of the uninfected versus infected snail samples. Changes in the lutein concentration of the infected DGG and whole snail bodies were insignificant compared to the uninfected controls. In conclusion, larval S. mansoni infection caused a significant decrease in the β-carotene concentration of the DGG at 6 and 8 weeks post infection.

Słowa kluczowe

EN

Biomphalaria glabrata; lutein; Schistosoma mansoni; infection; snail; high performance thin-layer chromatography; trematode; beta-carotene; digestive gland; densitometry

• Wydawca: -
• Czasopismo: Acta Parasitologica
• Rocznik: 2013
• Tom: 58
• Numer: 3
• Opis fizyczny: p.259-262,ref.

Twórcy

autor

Dieterich N.

• Program in Neuroscience, Lafayette College, Easton, PA, USA

autor

Fried B.

• Department of Biology, Lafayette College, Easton, PA, USA

autor

Sherma J.

• Department of Chemistry, Lafayette College, Easton, PA, USA

Bibliografia

• Cazzonelli C.I. 2011. Carotenoids in nature: insights from plants and beyond. Functional Plant Biology, 38, 833–847. DOI: 10.1071/FP11192.
• Evans R.T., Fried B., Sherma J. 2004. Effects of diet and larval trematode parasitism on lutein and β-carotene concentrations in planorbid snails as determined by quantitative high performance reversed phase thin layer chromatography. Comparative (Accepted March 18, 2013) Biochemistry and Physiology, Part B, 137, 179–186. DOI: 10.1016/j.cbpc.2003.11.003.
• Francis G.W., Anderson O.M. 2003. Natural pigments. In: Sherma J., Fried B. (Eds.), Handbook of Thin-Layer Chromatography. 3rd ed. Marcel Dekker, NY, 708–714 pp.
• Fried B., Holender S., Shetty P.H., Sherma J. 1990. Effects of Echinostoma trivolvis (Trematoda) infection on neutral lipids, sterols, and carotenoids in Helisoma trivolvis (Gastropoda). Comparative Biochemistry and Physiology, Part B, 97, 601–604.
• Marsit C.J., Fried B., Sherma J. 2000. High-performance thin-layer chromatographic analysis of lutein and β-carotene in Cerithidea californica (Gastropoda) infected with two species of larval trematodes. Journal of Parasitology, 86, 635–636. DOI: 10.1007/s004360050061.
• Sherma J., O’Hea C.M., Fried B. 1992. Separation, identification, and quantification of chloroplast pigments by HPTLC with scanning densitometry. Journal of Planar Chromatography-Modern TLC, 5, 343–349.
• Zeb A., Murkovic M. 2010. Thin-layer chromatographic analysis of carotenoids in plant and animal samples. Journal of Planar Chromatography-Modern TLC, 23, 35–39. DOI: 10.1556/JPC.23.2010.2.1.
• Ulmer M.J. 1970. Notes on rearing snails in the laboratory. In: MacInnis A.J., Voge M. (Eds.), Experiments and Techniques in Parasitology. W.H. Freeman, San Francisco, 143–144 pp.