Screening proteins interacting with VpPR10.1 of Chinese wild grapevine using the yeast two-hybrid system

• Autorzy: Xu T-F , Xiang J. , Li F.-J. , Li T.-M. , Yu Y.-H. , Wang Y.-J. , Xu Y.
• Języki publikacji: EN

Abstrakty

EN

Among the 17 plant pathogenesis-related (PR) protein families, only PR10 family is intracellular and cytosolic. PR proteins are expressed in response to pathogen challenge and abiotic stresses in higher plants. However, the molecular mechanisms of their actions remain poorly understood. In a previous work, we isolated a PR10 gene from Erysiphe necator-resistant Chinese wild Vitis sp. (Baihe-35-1) and it was designated as VpPR10.1. In this study, yeast two-hybrid method was used to screen proteins interacting with VpPR10.1 proteins. Twenty-one ESTs were isolated and sequenced. All sequences were compared using BLASTx to identify presumptive orthologs. Several proteins associated with VpPR10.1 protein were screened, including CNR8, UFGT6, HSP, DEAD-box, Trx h2, Grx C9 and GLOX. These proteins are closely related to defensive action of plants against pathogens and abiotic stresses. Our results reveal that VpPR10.1 gene may be involved in hormone signaling, programmed cell death and defense responses of grapevine.

• Wydawca: -
• Czasopismo: Acta Physiologiae Plantarum
• Rocznik: 2013
• Tom: 35
• Numer: 08
• Opis fizyczny: p.2355-2364,fig.,ref.

Twórcy

autor

Xu T-F

• State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China

autor

Xiang J.

• State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China

autor

Li F.-J.

• State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China

autor

Li T.-M.

• State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China

autor

Yu Y.-H.

• State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China

autor

Wang Y.-J.

• State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China

autor

Xu Y.

• State Key Laboratory of Crop Stress Biology in Arid Areas, Northwest A&F University, Yangling 712100, Shaanxi, China

Bibliografia

• AbuQarn M, Allmeling C, Amshoff I, Menger B, Nasser I, Vogt PM, Reimers K (2011) The yeast two hybrid system in a screen for proteins interacting with axolotl (Ambystoma mexicanum) Msx1 during early limb regeneration. Biochim Biophys Acta Proteins Proteom 1814:843–849
• Aon-Bertolino ML, Romero JI, Galeano P, Holubiec M, Badorrey MS, Saraceno GE, Hanschmann EM, Lillig CH, Capani F (2011) Thioredoxin and glutaredoxin system proteins-immunolocalization in the rat central nervous system. Biochim Biophys Acta Gen Subj 1810:93–110
• Asif MH, Dhawan P, Nath P (2000) A simple procedure for the isolation of high quality RNA from ripening banana fruit. Plant Mol Biol Rep 18:105–119
• Barnett T, Altschuler M, McDaniel CN, Mascarenhas JP (1980) Heat shock induced proteins in plant cells. Dev Genet 1:331–340
• Bell JN, Ryder TB, Wingate VPM, Bailey JA, Lamb CJ (1986) Differential accumulation of plant defense gene transcripts in a compatible and an incompatible plant-pathogen interaction. Mol Cell Biol 6:1615–1623
• Boch J, Verbsky ML, Robertson TL, Larkin JC, Kunkel BN (1998) Analysis of resistance gene-mediated defense responses in Arabidopsis thaliana plants carrying a mutation in CPR5. Mol Plant Microbe Interact 11:1196–1206
• Castro AJ, Carapito C, Zorn N, Magne C, Leize E, Dorsselaer AV, Clement C(2005) Proteomic analysis of grapevine (Vitis vinifera L.) tissues subjected to herbicide stress. J Exp Bot 56:2783–2795
• Choi DS, Hwang IS, Hwang BK (2012) Requirement of the cytosolic interaction between pathogenesis-related protein 10 and leucinerich repeat protein 1 for cell death and defense signaling in pepper. Plant Cell 2:1675–1690
• Fields S, Song O (1989) A novel genetic system to detect protein–protein interactions. Nature 340:245–246
• Ford CM, Boss PK, Hoj PB (1998) Cloning and characterization of Vitis vinifera UDP-glucose: flavonoid 3-O-glucosyltransferase, a homologue of the enzyme encoded by the maize Bronze-1 locus that may primarily serve to glucosylate anthocyanidins in vivo. J Biol Chem 273:9224–9233
• Guan X, Zhao H, Xu Y, Wang Y (2011) Transient expression of glyoxal oxidase from the Chinese wild grape Vitis pseudoreticulata can suppress powdery mildew in a susceptible genotype. Protoplasma 248:415–423
• Hammond-Kosack KE, Jones JD (1996) Resistance gene-dependent plant defense responses. Plant Cell 8:1773–1791
• Hammond-Kosack KE, Parker JE (2003) Deciphering plant-pathogen communication: fresh perspectives for molecular resistance breeding. Curr Opin Biotech 14:177–193
• Holmgren A (1978) Glutathione-dependent enzyme reactions of the phage T4 ribonucleotide reductase system. J Biol Chem 253:7424–7430
• Jarmoskaite I, Russell R (2011) DEAD-box proteins as RNA helicases and chaperones. Wiley Interdiscip Rev RNA 2:135–152
• Jellouli N, Jouira BH, Skouri H, Ghorbel A, Gourgouri A, Mliki A (2008) Proteomic analysis of Tunisian grapevine cultivar Razegui under salt stress. J Plant Physiol 165:471–481
• Kang K, Guo K, Tang Q, Zhang Y, Wu J, Li W, Lin Z (2012) Interactive cellular proteins related to classical swine fever virus non-structure protein 2 by yeast two-hybrid analysis. Mol Biol Rep 39:10515–10524
• Karlsson AL, Alm R, Ekstrand B, Fjelkner-Modig S, Schiött A, Bengtsson U, Björk L, Hjernø K, Roepstorff P, Emanuelsson CS
• (2004) Bet v 1 homologues in strawberry identified as IgE-binding proteins and presumptive allergens. Allergy 59:1277–1284
• Kersten PJ, Cullen D (1993) Cloning and characterization of a cDNA encoding glyoxal oxidase, a hydrogen peroxide-producing enzyme from the lignin-degrading basidiomycete Phanerochaete chrysosporium. Proc Natl Acad Sci USA 90:7411–7413
• Key JL, Lin CY, Chen YM (1981) Heat shock proteins of higher plants. Proc Natl Acad Sci USA 78:3526–3530
• Kim YY, Jung KW, Jeung JU, Shin JS (2012) A novel F-box protein represses endothecial secondary wall thickening for anther dehiscence in Arabidopsis thaliana. J Plant Physiol 169:212–216
• Kirik V, Bouyer D, Schobinger U, Bechtold N, Herzog M, Bonneville JM, Hulskamp M (2001) CPR5 is involved in cell proliferation and cell death control and encodes a novel transmembrane protein. Curr Biol 11:1891–1895
• Leuthner B, Aichinger C, Oehmen E, Koopmann E, Muller O, Muller P, Kahmann R, Bolker M, Schreier PH (2005) A H2O2-producing glyoxal oxidase is required for filamentous growth and pathogenicity in Ustilago maydis. Mol Genet Genomics 272:639–650
• Lillig CH, Holmgren A (2007) Thioredoxin and related molecules—from biology to health and disease. Antioxid Redox Signal 9:25–47
• Linder P (2006) Dead-box proteins: a family affair-active and passive players in RNP-remodeling. Nucleic Acids Res 34:4168–4180
• Linder P, Jankowsky E (2011) From unwinding to clamping—the DEAD box RNA helicase family. Mol Cell Biol 12:505–516
• Liu JJ, Ekramoddoullah AKM (2006) The family 10 of plant pathogenesis-related proteins: their structure, regulation, and function in response to biotic and abiotic stresses. Physiol Mol Plant Pathol 68:3–13
• Lo SSC, Nicholson RL (1998) Reduction of light-induced anthocyanin accumulation in inoculated sorghum mesocotyls. Plant Physiol 116:979–989
• Malladi A, Hirst PM (2010) Increase in fruit size of a spontaneous mutant of ‘Gala’ apple (Malus 9 domestica Borkh.) is facilitated by altered cell production and enhanced cell size. J Exp Bot 61:3003–3013
• Markovic-Housely Z, Degano M, Lamba D, Roepenack-Lahaye E, Clemens S, Susani M, Ferreira F, Scheiner O, Breiteneder H (2003) Crystal structure of a hypoallergenic isoforms of the major birch pollen allergen Bet v 1 and its likely biological function as a plant steroid carrier. J Mol Biol 325:123–133
• Mehlen P, Schulze-Osthoff K, Arrigo AP (1996) Small stress proteins as novel regulators of apoptosis. Heat shock protein 27 blocks Fas/APO-1- and staurosporine-induced cell death. J Biol Chem 271:16510–16514
• Mehlen P, Mehlen A, Godet J, Arrigo AP (1997) hsp27 as a switch between differentiation and apoptosis in murine embryonic stem cells. J Biol Chem 272:31657–31665
• Musidlowska-Persson A, Alm R, Emanuelsson C (2007) Cloning and sequencing of the Bet v 1-homologous allergen Fra a 1 in strawberry (Fragaria ananassa) shows the presence of an intron and little variability in amino acid sequence. Mol Immunol 44:1245–1252
• Schenk MF, Gilissen LJ, Esselink GD, Smulders MJ (2006) Seven different genes encode a diverse mixture of isoforms of Bet v1, the major birch pollen allergen. BMC Genomics 7:168
• Somssich IE, Schmelzer E, Bollmann J, Hahlbrock K (1986) Rapid activation by fungal elicitor of genes encoding pathogenesisrelated proteins in cultured parsley cells. Proc Natl Acad Sci USA 83:2427–2430
• Stamp N (2003) Out of the quagmire of plant defense hypotheses. Q Rev Biol 78:23–55
• Torres MA, Jones JDG, Dangl JL (2006) Reactive oxygen species signaling in response to pathogens. Plant Physiol 141:373–378
• Van Loon LC, Van Strien EA (1999) The families of pathogenesisrelated proteins, their activities, and comparative analysis of PR- 1 type proteins. Physiol Mol Plant Pathol 55:85–97
• Wang YJ, Liu Y, He P, Chen J, Lamicanra O, Lu J (1995) Evaluation of foliar resistance to Uncinula necator in Chinese wild Vitis species. Vitis 34:159–164
• Xu Y, Yu H, He M, Yang Y, Wang Y (2010) Isolation and expression analysis of a novel pathogenesis-related protein 10 gene from Chinese wild Vitis pseudoreticulata induced by Uncinula necator. Biologia 65:653–659

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