First detection and molecular characterization of kobuvirus from a dog in Turkey

• Autorzy: Akkutay-Yoldar Z. , Koc B.T. , Oguzoglu T.C.
• Języki publikacji: EN

Abstrakty

EN

Canine kobuvirus (CaKVs) is a newly emerging virus detected in dogs in several countries. However, kobuvirus infection has not yet been described in domestic carnivores in Turkey. In this study, we tested blood and rectal swab samples to determine the presence of kobuvirus in a dog with clinical symptoms by reverse transcription-polymerase chain reaction (RT-PCR), using 3D (RNA polymerase) region primers of canine kobuviruses. To provide molecular characterization data, the Maximum Likelihood (ML) method was used for the phylogenetic analyses. The PCR product of the partial protein-coding region of the 3D protein gene from the rectal swab was amplified, purified, and sequenced. Phylogenetic analysis of amino acid sequences suggests that our CaKV strain was closely related to US-CaKVs,and placed on a monophyletic clade as a sister branch localized in the CaKV cluster. These results indicate that CaKV exists in dogs in Turkey. With a similarity of 94.2–96.1%, it is like other CaKVs. To our knowledge, this is the first report of CaKV infection of a dog by in Turkey. Further studies are needed to determine its role in dog gastrointestinal infections.

• Wydawca: -
• Czasopismo: Medycyna Weterynaryjna
• Rocznik: 2019
• Tom: 75
• Numer: 11
• Opis fizyczny: p.673-677,fig.,ref.

Twórcy

autor

Akkutay-Yoldar Z.

• Department of Virology, Faculty of Veterinary Medicine, Ankara University, Diskapi 06110, Ankara, Turkey

autor

Koc B.T.

• Department of Virology, Faculty of Veterinary Medicine, Aydın Adnan Menderes University, 09016, Aydın, Turkey

autor

Oguzoglu T.C.

• Department of Virology, Faculty of Veterinary Medicine, Ankara University, Diskapi 06110, Ankara, Turkey

Bibliografia

• Amimo J. O., Okoth E., Junga J. O., Ogara W. O., Njahira M. N., Wang Q., Vlasova A. N., Saif L. J., Djikeng A.: Molecular detection and genetic characterization of kobuviruses and astroviruses in asymptomatic local pigs in East Africa. Arch. Virol. 2014, 159, 1313-1319.
• Carmona-Vicente N., Buesa J., Brown P. A., Merga J. Y., Darby A. C., Stavisky J., Sadler R. M., Gaskell S., Dawson A. D.: Phylogeny and prevalence of kobuviruses in dogs and cats in the UK. Vet. Microbiol. 2013, 164, 246-252.
• Chomczynski P., Sacchi N.: Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 1987, 162, 156-159.
• Chung J. Y., Kim S. H., Kim Y. H., Lee M. H., Lee K. K., Oem J. K.: Detection and genetic characterization of feline kobuviruses. Virus Genes 2013, 47, 559-562.
• Di Martino B., Di Felice E., Ceci C., Di Profio F., Marsilio F.: Canine kobuviruses in diarrhoeic dogs in Italy. Vet. Microbiol. 2013, 166, 246-249.
• Greninger A. L., Knudsen G. M., Betegon M., Burlingame A. L., Derisi J. L.: The 3A protein from multiple picornaviruses utilizes the golgi adaptor protein ACBD3 to recruit PI4KIIIβ. J. Virol. 2012, 86, 3605-3616.
• Kapoor A., Simmonds P., Dubovi E. J., Qaisar N., Henriquez J. A., Medina J., Shelly S., Lipkin W. I.: Characterization of a canine homolog of human Aichivirus. J. Virol. 2011, 85, 11520-11525.
• Kong N., Zuo Y., Wang Z., Yu H., Zhou E. M., Shan T., Tong G.: Molecular characterization of new described kobuvirus in dogs with diarrhea in China. Springerplus 2016, 5, 2047.
• Lee M. H., Jeoung H. Y., Lim J. A., Song J. Y., Song D. S., An D. J.: Kobuvirus in South Korean black goats. Virus Genes 2012, 45, 186-189.
• Li L., Pesavento P. A., Shan T., Leutenegger C. M., Wang C., Delwart E.: Viruses in diarrhoeic dogs include novel kobuviruses and sapoviruses. J. Gen. Virol. 2011, 92, 2534.
• Muhire B. M., Varsani A., Martin D. P.: SDT: a virus classification tool based on pairwise sequence alignment and identity calculation. PlOS One 2014, 9, e108277.
• Oem J. K., Choi J. W., Lee M. H., Lee K. K., Choi K. S.: Canine kobuvirus infections in Korean dogs. Arch. Virol. 2014, 159, 2751-2755.
• Oh D. Y., Silva P. A., Hauroeder B., Diedrich S., Cardoso D. D., Schreier E.: Molecular characterization of the first Aichi viruses isolated in Europe and in South America. Arch. Virol. 2006, 151, 1199-1206.
• Pankovics P., Boros Á., Kiss T., Reuter G.: Identification and complete genome analysis of kobuvirus in faecal samples of European roller (Coracias garrulus): for the first time in a bird. Arch. Virol. 2015, 160, 345-351.
• Phan T., Kapusinszky B., Wang C., Rose P. K., Lipton H. L., Delwart E.: The fecal viral flora of wild rodents. PLOS Pathog. 2011, 7, p. e1002218.
• Reuter G., Boldizsár Á., Pankovics P.: Complete nucleotide and amino acid sequences and genetic organization of porcine kobuvirus, a member of a new species in the genus Kobuvirus, family Picornaviridae. Arch. Virol. 2009, 154, 101.
• Smits S. L., Raj V. S., Oduber M. D., Schapendonk C. M., Bodewes R., Provacia L., Stittelaar K. J., Osterhaus A. D., Haagmans B. L.: Metagenomic analysis of the ferret fecal viral flora. PLOS One 2013, 8, p. e71595.
• Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.: MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Mol. Biol. Evol. 2013, 30, 2725-2729.
• Yamashita T., Shinichi K., Kcnji S., Shuji N., Shunzo C., Yuichi I., Shin I.: Isolation of cytopathic small round viruses with BS-C-1 cells from patients with gastroenteritis. J. Infect. Dis. 1991, 164, 954-957.
• Yamashita T., Ito M., Kabashima Y., Tsuzuki H., Fujiura A., Sakae K.: Isolation and characterization of a new species of kobuvirus associated with cattle. J. Gen. Virol. 2003, 84, 3069-3077.

Identyfikatory

DOI

10.21521/mw.6248