The effect of inoculating single cultures of plant growth-promoting rhizobacteria (PGPR) on development of white beans seedling (Phaseolus vulgaris) was analyzed. Five PGPR were isolated from the rhizosphere of Okra plants and were assessed for abilities to solubilise phosphate and produce indole acetic acid (IAA). The phosphate solubilising index ranged from 6-10 while the concentration of IAA ranged from 17.48mg/l to 27.43mg/l. Serratia sp. produced the highest concentration of IAA (27.43mg/l) and had the highest solubilisation index (10mm). Bacillus sp. produced the least amount of IAA (17.48mg/l) while Staphylococcus sp. had the least solubilisation index (6mm). The highest percentage germination of 83.3% was observed in the seedling inoculated with Bacillus sp. The effect of PGPR on root and shoot elongation was studied hydroponically for 7 days. Significant increases (P< 0.05) in root elongation were observed.. The highest seedling root length (18.47cm) and shoot length (19.17cm) were observed with inoculation of Staphylococcus sp. and Bacillus sp. respectively. The use of these bacteria as bio-inoculants could be a sustainable practice to facilitate nutrient supply to white beans seedlings.
Department of Microbiology, Federal University of Technology Owerri, Imo State, Nigeria
Bibliografia
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DOI References
[1] V.K. Kumar, M.S. Reddy, J.W. Kloepper, K.S. Lawrence, X.G. Zhou, D.E. Groth, S. Zhang, R.R. Sudhakara, Q. Wang, M.R.B. Raju, R. Krishnam, F.W.G. Dilantha, H. Sudini, B. Du and M.E. Miller, Commercial potential of microbial inoculants for sheath blight management and yield enhancement of rice. In: Maheshwari D.K. (ed) bacteria in agrobiology: crop ecosystems springer, Berlin pp.237-264. (2011). 10.1007/978-3-642-18357-7_9
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[3] M.V.B. Figueiredo, H.A. Burity, C.R. Martinez and C.P. Chanway, Plant growth-promoting rhizobacteria for improving nodulation and nitrogen fixation in the common bean (Phaseolus vulgaris L. ). World J. Microbiol. Biotech. 24, (2007)1187-1193. 10.1007/s11274-007-9591-4
[4] S.G. Dastager, C.K. Deepa and A. Pandey, Isolation and characterization of novel plant growth promoting rhizobacteria Micrococcus sp. N11-0909 and its interaction with cow-pea. Plant physiol. Biochem. 48(12), (2010)987-992. 10.1016/j.plaphy.2010.09.006
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[9] Y.P. Chen, P.D. Rekha, A. B Arun, F.T. Shen, W.A. Lai and C.C. Young,. Phosphate solubilizing bacteria from subtropical soil and their tricalcium phosphate solubilising abilities. App. Soil Ecol. 34, (2006) 33-41. 10.1016/j.apsoil.2005.12.002
[10] H. Antoun, Beneficial microorganisms for the sustainable use of phosphates in agriculture. Procedia Eng. 46, (2012) 62-67. 10.1016/j.proeng.2012.09.446
[11] B. Hameeda, O.P. Rupela, G. Reddy and K. Satyavani, Application of PRPB associated with composts and macrofauna for growth promotion of pearl millet (Pennisetum glaucum L. ). Biol. Fert. Soil 43 (2), (2006)221-227.10.1007/s00374-006-0098-1
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