The effect of experimental low zearalenone intoxication on ovarian follicles in pre-pubertal bitches

• Autorzy: Gajecka M.
• Języki publikacji: EN

Abstrakty

EN

Companion animals, including bitches, may be exposed to zearalenone (ZEN) toxins that are often present in feed, and ZEN intoxication may lead to ovarian dysfunction. This study involved evaluation of the degree of ZEN-induced hypo stimulation of ovary by determination of proliferative and apoptotic indices and description of the ultra-structural organization of ovarian follicles in pre-pubertal bitches subjected to experimental, long-term exposure to low-dose ZEN mycotoxicosis. The experiment involved 30 clinically healthy, immature Beagle bitches aged approximately 70 days with initial average body weight of 8 kg, randomly divided into three groups of 10 animals each: two experimental groups (EI and EII) and a control group (C). Over a period of 42 days, ZEN was administered per os to EI animals at a dose of 50 μg/kg BW, and to EII bitches at a dose of 75 μg/kg BW. Control group animals were fed placebo containing no ZEN for 42 days. Analytical samples of the mycotoxin were administered daily in gelatin capsules before morning feeding. All the bitches were subjected to ovario-hysterectomy at the end of the experiment. Proliferation index (PCNA method) and apoptotic index (TUNEL test) values were determined by immune-histological analyses. The median for apoptotic index was determined at 13.45 for group EI, 17.84 for group EII, and 8.59 for group C. The median for proliferation index was determined at 35.25 for group EII, 42.44 for group EI, and 70.60 for group C. The results of ultra-structural examinations of the ovaries revealed that experimental, ZEN-induced hyperestrogenism enhanced apoptosis and lowered the proliferative ability of follicular cells what contributed to organelle destruction in pre-pubertal bitches. The changes observed were particularly advanced in EII animals, which were administered a higher dose of ZEN.

• Wydawca: -
• Czasopismo: Polish Journal of Veterinary Sciences
• Rocznik: 2013
• Tom: 16
• Numer: 1
• Opis fizyczny: p.45-54,fig.,ref.

Twórcy

autor

Gajecka M.

• Department of Veterinary Prevention and Feed Hygiene, Faculty of Veterinary Medicine, University of Warmia and Mazury in Olsztyn, Oczapowskiego 13, 10-718 Olsztyn, Poland

Bibliografia

• Boermans HJ, Leung MC (2007) Mycotoxins and the pet food industry: toxicological evidence and risk assessment. Int J Food Microbiol 119: 95-102.
• Chen JQ, Yager JD, Russo J (2005) Regulation of mitochondrial respiratory chain structure and function by estrogens/estrogen receptors and potential physiological/ pathophysiological implications. Biochim Biophys Acta 1746: 1-17.
• Concannon PW (2011) Reproductive cycles of the domestic bitch. Anim Reprod Sci 124: 200-210.
• De Bosschere H, Ducatelle R, Tshamala M, Coryn M (2002) Changes in sex hormone receptors during administration of progesterone to prevent estrus in the bitch. Theriogenology 58: 1209-1217.
• Doll S, Danicke S (2011) The Fusarium toxins deoxynivalenol (DON) and zearalenone (ZON) in animal feeding. Prev Vet Med 102: 132-145.
• Dworakowska D (2005) Apoptotic index and selected cell cycle regulators in non-small cell lung cancer. Ann Acad Med Gedanensis 35: 1-112.
• Felty Q, Roy D (2005) Estrogen, mitochondria and growth of cancer and non-cancer cells. J Carcinog 4: 1.
• Gajęcka M (2012) The effect of low-dose experimental zearalenone intoxication on the immunoexpression of estrogen receptors in the ovaries of pre-pubertal bitches. Pol J Vet Sci 15: 685-691.
• Gajęcka M, Jakimiuk E, Polak M, Otrocka-Domagała I, Janowski T, Zwierzchowski W Obremski K, Zielonka Ł, Apoznański J, Gajęcki M (2004) Zearalenone applied per os provides adverse effects in structure of chosen parts of bitch reproductive system. Pol J Vet Sci 7: 59-66.
• Gajęcka M, Jakimiuk E, Zielonka Ł, Obremski K, Gajęcki M (2009) The biotransformation of chosen mycotoxins. Pol J Vet Sci 12: 293-303.
• Gajęcka M, Obremski K, Jakimiuk E, Skorska-Wyszyńska E, Zielonka Ł, Gajęcki M (2008) Histopathological examination of ovaries in bitches after experimental zearalenone micotoxicosis. Pol J Vet Sci 11: 363-366.
• Gajęcka M, Przybylska-Gornowicz B (2012) The low doses effect of experimental zearalenone (ZEN) intoxication on the presence of Ca2+ in selected ovarian cells from pre-pubertal bitches. Pol J Vet Sci 15: 711-720.
• Gajęcka M, Rybarczyk L, Zwierzchowski W, Jakimiuk E, Zielonka Ł, Obremski K, Gajęcki M (2011) The effect of experimental, long-term exposure to low-dose zearalenone mycotoxicosis on the histological condition of ovaries in sexually immature gilts. Theriogenology 75: 1085-1094.
• Gajęcki M, Gajęcka M, Jakimiuk E, Zielonka Ł, Obremski K (2010) Zearalenone – undesirable substance. In: Mahendra R, Ajit V (eds) Mycotoxins in food, feed and bioweapons. Springer-Verlag Berlin Heidelberg, pp. 131-144.
• Jiang Z, Fletcher NM, Ali-Fehmi R, Diamond MP, Abu-Soud HM, Munkarah AR, Saed GM (2011) Modulation of redox signaling promotes apoptosis in epithelial ovarian cancer cells. Gynecol Oncol 122: 418-423.
• Moreira PI, Custódio JB, Nunes E, Oliveira PJ, Moreno A, Seica R, Oliveira CR, Santos MS (2011) Mitochondria from distinct tissues are differently affected by 17β-estradiol and tamoxifen. J Steroid Biochem Mol Biol1 23: 8-16.
• Motea EA, Berdis AJ (2010) Terminal deoxynucleotidyl transferase: the story of a misguided DNA polymerase. Biochim Biophys Acta 1804: 1151-1166.
• Nadal A, Alonso-Magdalena P, Ripoll C, Fuentes E (2005) Disentangling the molecular mechanisms of action of endogenous and environmental estrogens. Pflug Arch Eur J Phy 449: 335-343.
• Obremski K, Gajęcki M, Zwierzchowski W, Bakuła T, Apoznański J, Wojciechowski J (2003) The level of zearalenone and alpha-zearalenol in the blood of gilts with clinical symptoms of toxicosis, fed diets with a low zearalenone content. J Anim Feed Sci 12: 529-538.
• Ranzenigo G, Caloni F, Cremonesi F, Aad PY, Spicer LJ (2008) Effects of Fusarium mycotoxins on steroid production by porcine granulosa cells. Anim Reprod Sci 107: 115-130.
• Serafim MKB, Araujo VR, Silva GM, Duarte ABG, Almeida AP, Chaves RN, Campello CC, Lopes CAP, de Figueiredo JR, Machado da Silva LD (2010) Canine preantral follicles cultured with various concentrations of follicle-stimulating hormone (FSH). Theriogenology 74: 749-755.
• Skierski J (2008) Cytotoxic assays of chemical substances. Post Biol Kom 35: 147-163.
• Songsasen N, Wildt DE (2007) Oocyte biology and challenges in developing in vitro maturation systems in the domestic dog. Anim Reprod Sci 98: 2-22.
• Takemura H, Shim JY, Sayama K, Tsubura A, Zhu BT, Shimoi K (2007) Characterization of the estrogenic activities of zearalenone and zeranol in vivo and in vitro. J Steroid Biochem Mol Biol 103: 170-177.
• Tilly JL (2001) Commuting the death sentence: how oocytes strive to survive. Nat Rev Mol Cell Biol 2: 838-848.
• Van Cruchten S, Van den Broeck W, D’haeseleer M, Simoens P (2004) Proliferation patterns in the canine endometrium during the estrous cycle. Theriogenology 62: 631-641.
• Vlata Z, Porichis F, Tzanakakis G, Tsatsakis A, Krambovitis E (2006) A study of zearalenone cytotoxicity on human peripheral blood mononuclear cells. Toxicol Lett 165: 274-281.
• Watson CS, Alyea RA, Jeng Y-J, Kochukov MY (2007) Nongenomic actions of low concentration estrogens and xenoestrogens on multiple tissues. Mol Cell Endocrinol 274: 1-7.
• Warzych E, Lechniak D (2005) Apoptosis in oogenesis and preimplantation embryo development of mammals. Biotechnologia 1: 172-182.
• Zeuner A, Muller K, Reguszynski K, Jewgenow K (2003) Apoptosis within bovine follicular cells and its effect on oocyte development during in vitro maturation. Theriogenology 59: 1421-1433.
• Zwierzchowski W, Gajęcki M, Obremski K, Zielonka Ł, Baranowski M (2004) The occurrence of zearalenone and its derivatives in standard and therapeutic feeds for companion animals. Pol J Vet Sci 7: 289-293.

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