Preferencje help
Widoczny [Schowaj] Abstrakt
Liczba wyników
2014 | 23 | 2 |

Tytuł artykułu

A rapid HPLC method for the determination of sulphonamides and trimethoprim in feed premixes

Warianty tytułu

Języki publikacji



A simple HPLC method for the simultaneous determination of sulphadimidine, sulphadiazine, sulphamethoxazole and trimethoprim antibiotics in medicated feeds has been developed, validated, and applied to commercial feed premixes. Ultrasound-assisted extraction was used for the recovery of the studied antibiotics without the need for a further clean-up step. The sulphonamides and trimethoprim were separated on a Symmetry C18 (5 μm, 4.6 × 250 mm) column using 10-min isocratic elution and UV detection at 254 nm. The results showed that the method is robust with adequate recovery (minimum 95.19%), satisfactory repeatability (1.39%–2.69%) and intermediate precision (1.36%–4.16%), as shown by the validation assessment herein. The advantages of the proposed method are the speed, the ease of the clean-up protocol, and the low cost of the needed instrumentation, rendering it highly applicable to the feed industry.

Słowa kluczowe








Opis fizyczny



  • Department of Agricultural Sciences, Biotechnology and Food Science, University of Technology, Limassol, P.O Box 50329, Cyprus
  • VitaTrace Nutrition Ltd, P.O Box 23886, Nicosia, Cyprus
  • VitaTrace Nutrition Ltd, P.O Box 23886, Nicosia, Cyprus
  • Department of Agricultural Sciences, Biotechnology and Food Science, University of Technology, Limassol, P.O Box 50329, Cyprus


  • Boscher A., Guignard C., Pellet T., Hoffmann L., Bohn T., 2010. Development of a multi-class method for the quantification of veterinary drug residues in feedingstuffs by liquid chromatography-tandem mass spectrometry. J. Chromatogr. A 1217, 6394–6404
  • Cahill J.D., Furlong E.T., Burkhardt M.R., Kolpin D., Anderson L.G., 2004. Determination of pharmaceutical compounds in surfaceand ground-water samples by solid-phase extraction and highperformance liquid chromatography-electrospray ionization mass spectrometry. J. Chromatogr. A 1041, 171–180
  • Croubels S., Wassink P., De Backer P., 2002. Simultaneous determination of sulfadiazine and trimethoprim in animal feed by liquid chromatography with UV and tandem mass spectrometric detection. Anal. Chim. Acta 473, 183–194
  • de Paula F.C.C.R., de Pietro A.C., Cass Q.B., 2008. Simultaneous quantification of sulfamethoxazole and trimethoprim in whole egg samples by column-switching high-performance liquid chromatography using restricted access media column for on-line sample clean-up. J. Chromatogr. A 1189, 221–226
  • DeAngelis D.V., Wooley J.L., Sigel C.W., 1990. High-performance liquid chromatographic assay for the simultaneous measurement of trimethoprim and sulfamethoxazole in plasma or urine. Ther. Drug Monit. 12, 382–392
  • European Commission, 2010. Evaluation of the EU Legislative Framework in the Field of Medicated Feed. Food Chain Evaluation Consortium (Editors). Final Report, Brussels, pp. 188
  • Farooq M.U., Su P., Yang Y., 2009. Applications of a novel sample preparation method for the determination of sulfonamides in edible meat by CZE. Chromatographia 69, 1107–1111
  • Grashorn M.A., 2010. Use of phytobiotics in broiler nutrition – An alternative to infeed antibiotics? J. Anim. Feed Sci. 19, 338–347
  • Jiménez V., Companyó R., Guiteras J., 2009. Preparation of quality control materials for the determination of sulfonamides in animal feed. Food Addit. Contam. A 26, 969–977
  • Kumar P., Companyo R., 2011. Development and validation of an LCUV method for the determination of sulfonamides in animal feeds. Drug Test. Anal. 4, 368–375
  • Link R., Kovác G., Pistl J., 2005. A note on probiotics as an alternative for antibiotics in pigs. J. Anim. Feed Sci. 14, 513–519
  • Liu R., He P., Li Z., Li R., 2011. Simultaneous determination of 16 sulfonamides in animal feeds by UHPLC–MS–MS. J. Chromatogr. Sci. 49, 640–646
  • Lopes R.P., De Freitas Passos T.E., De Alkimim Filho J.F., Vargas E.A., Augusti D.V., Augusti R., 2012. Development and validation of a method for the determination of sulfonamides in animal feed by modified QuEChERS and LC-MS/MS analysis. Food Control 28, 192–198
  • McClure E.L., Wong C.S., 2007. Solid phase microextraction of macrolide, trimethoprim, and sulfonamide antibiotics in wastewaters. J. Chromatogr. A 1169, 53–62
  • Renew J.E., Huang C.-H., 2004. Simultaneous determination of fluoroquinolone, sulfonamide, and trimethoprim antibiotics in wastewater using tandem solid phase extraction and liquid chromatography-electrospray mass spectrometry. J. Chromatogr. A 1042, 113–121
  • Samanidou V.F., Evaggelopoulou E.N., 2008. Chromatographic analysis of banned antibacterial growth promoters in animal feed. J. Sep. Sci. 31, 2091–2112
  • Silva F.E.B., Ferrão M.F., Parisotto G., Müller E.I., Flores E.M.M., 2009. Simultaneous determination of sulphamethoxazole and trimethoprim in powder mixtures by attenuated total reflection-Fourier transform infrared and multivariate calibration. J. Pharmaceut. Biomed. Anal. 49, 800–805
  • Sun H., Ai L., Wang F., 2007. Quantitative analysis of sulfonamide residues in natural animal casings by HPLC. Chromatographia 66, 333–337
  • Teshima D., Otsubo K., Makino K., Itoh Y., Oishi, R., 2004. Simultaneous determination of sulfamethoxazole and trimethoprim in human plasma by capillary zone electrophoresis. Biomed. Chromatogr. 18, 51–54
  • Turnidge J., 2004. Antibiotic use in animals - Prejudices, perceptions and realities. J. Antimicrob. Chemother. 53, 26–27

Typ dokumentu



Identyfikator YADDA

JavaScript jest wyłączony w Twojej przeglądarce internetowej. Włącz go, a następnie odśwież stronę, aby móc w pełni z niej korzystać.