Cadherin and vimentin immunoexpression in the testis of normal and induced infertility models of albino rats

• Autorzy: ElGhamrawy T.A. , Helmy D. , Elall H.F.A.
• Języki publikacji: EN

Abstrakty

EN

Background: Sertoli cells are important in determining the fate of spermatogenic cells by providing nutrition and structural support via cell junctions. Adhesion between Sertoli and germ cells is important for spermatogenesis. Cadherin are transmembrane proteins that mediate cell-cell adhesion, while, vimentin, the cytoskeletal intermediate filament plays an important role in spermatogenesis. The aim of the present study was to investigate cadherin and vimentin immunoexpression in the normal testis and in two types of altered spermatogenic states: the cyclophosphamide (CP) treatment and the cryptorchidism (Cx) models. Materials and methods: Twenty four male albino rats were divided into control group: 6 rats receiving saline orally and the other 6 were sham-operated. CP group (n = 6): given 6 mg/kg/day of CP orally for 4 weeks. Cx group (n = 6): the left testis was surgically freed from the scrotum and fixed in the abdomen. Animals were sacrificed and the left testis dissected and prepared to be stained with haematoxylin and eosin stain and immunohistochemical stain against cadherin and vimentin. Morphometric measurements and statistical analysis were done. Results: In CP-treated group there was degeneration of spermatocytes, vacuolations of Sertoli cells and absence of spermatozoa. These changes were more prominent in Cx group, in addition to interstitial hypercellularity. There was also a significant decrease in cadherin and vimentin immunostaining in CP-treated group that was more marked in the cryptorchidism group. Conclusions: A downregulation of both cadherin and vimentin was associated with both models of impaired spermatogenesis. This impairment could be attributed to disruption of the junctions between Sertoli and germ cells. (Folia Morphol 2014; 73, 3: 339–346)

• Wydawca: -
• Czasopismo: Folia Morphologica
• Rocznik: 2014
• Tom: 73
• Numer: 3
• Opis fizyczny: p.339-346,fig.,ref.

Twórcy

autor

ElGhamrawy T.A.

• Department of Anatomy, Faculty of Medicine, Cairo University, Cairo, Egypt

autor

Helmy D.

• Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt

autor

Elall H.F.A.

• Department of Histology, Faculty of Medicine, Cairo University, Cairo, Egypt

Bibliografia

• 1. Absalan F, Movahedin M, Mowla SJ (2009) Germ cell apoptosis induced by experimental cryptorchidism is mediated by molecular pathways in mouse testis. Andrologia, 42: 5–12.
• 2. Alam M, Ohsako T, Tay T, Tsunekawa N, Kanai Y, Kurohmaru M (2010) Di (n-butyl) phthalate induces vimentin filaments disruption in rat Sertoli cells: a possible relation with spermatogenic cell apoptosis. Anat Histol Embryol, 39: 186–193.
• 3. Amlani S, Vogl AW (1988) Changes in the distribution of microtubules and intermediate filaments in mammalian Sertoli cells during spermatogenesis. Anat Rec, 220: 143–160.
• 4. Bancroft JD, Gamble M (2002) Theory and practice of histological techniques. 5th Ed. Churchill Livingstone, London, UK, p. 130.
• 5. Chen M, Cai H, Yang JL, Lu CL, Liu T, Yang W, Guo J, Hu XQ, Fan CH, Hu ZY, Gao F, Liu YX (2008) Effect of heat stress on expression of junction-associated molecules and upstream factors androgen receptor and Wilms’ tumor1 in monkey Sertoli cells. Endocrinology, 149: 4871–4882.
• 6. Cheng C, Mruk D (2002) Cell junction dynamics in the testis: Sertoli-germ cell interactions and male contraceptive development. Physiological Rev, 82: 825–874.
• 7. Codrington AM, Hales BF, Robaire B (2007) Chronic cyclophosphamide exposure alters the profile of rat sperm nuclear matrix proteins. Biol Reproduction, 77: 303–311.
• 8. Devkota B, Sasaki M, Matsui M, Amaya Montoya C, Miyake Y (2006) Alterations in the immunohistochemical localization patterns of alpha-smooth muscle actin (SMA) and vimentin in the postnatally developing bovine cryptorchid testis. J Reprod Dev, 52: 329–334.
• 9. Drumond AL, Weng CC, Wang G, Chiarini-Garcia H, Eras-Garcia L, Meistrich ML (2011) Effects of multiple doses of cyclophosphamide on mouse testes: accessing the germ cells lost, and the functional damage of stem cells. Reproductive Toxicol, 32: 395–406.
• 10. Hejmej A, Kopera I, Kotula-Balak M, Lydka M, Lenartowicz M, Bilinska B (2012) Are expression and localization of tight and adherens junction proteins in testes of adult boar affected by foetal and neonatal exposure to flutamide? Int J Andrology, 35: 340–352.
• 11. Hoorweg-Nijman JJ, Delemarre-van de Waal HA, de Waal FC, Behrendt H (1992) Cyclophosphamide-induced disturbance of gonadotropin secretion manifesting testicular damage. Acta Endocrinol, 126: 143–148.
• 12. Jiang J, Dean D, Burghardt R, Parrish A (2004) Disruption of cadherin/catenin expression, localization, and interactions during HgCl2-induced nephrotoxicity. Toxicol Scien, 80: 170–182.
• 13. Johnson KJ, Boekelheide K (2002) Dynamic testicular adhesion junctions are immunologically unique. I. Localization of p120 catenin in rat testis. Biol Reproduction, 66: 983–991.
• 14. Johnson KJ, Zecevic A, Kwon EJ (2004) Protocadherin a3 acts at sites distinct from classic cadherins in rat testis and sperm. Biol Reproduction, 70: 303–312.
• 15. Kolasa A, Marchlewicz M, Wenda-Różewicka L, Wiszniewska B (2011) DHT deficiency perturbs the integrity of the rat seminiferous epithelium by disrupting tight and adherens junctions. Folia Histoch Cytobiol, 49: 62–71.
• 16. Kopecky M, Semecky V, Nachtigal P (2005) Vimentin expression during altered spermatogenesis in rats. Acta Histochemica, 107: 279–289.
• 17. Liu F, Li XL, Lin T, He DW, Wei GH, Liu JH, Li LS (2012) The cyclophosphamide metabolite, acrolein, induces cytoskeletal changes and oxidative stress in Sertoli cells. Mol Biol Reprod, 39: 493–500.
• 18. Manda K, Bhatia A (2003) Prophylactic action of melatonin against cyclophosphamide-induced oxidative stress in mice. Cell Biol Toxicol, 19: 367–372.
• 19. Petrie A, Sabin C (2005) Medical statistics at a glance. In: Sugden M, Moore K eds. 2nd Ed. Blackwell Publishing Ltd., USA, p. 55.
• 20. Pospechova K, Kopecky M, Nachtigal P, Pospisilova N, Jamborova G, Semecky V (2007) Changes in the expression of P-cadherin in the normal, cryptorchid and busulphan-treated rat testis. Int J Andrology, 30: 430–438.
• 21. Ren L, Medan MS, Ozu M, Li C, Watanabe G, Taya K (2006) Effects of experimental cryptorchidism on sperm motility and testicular endocrinology in adult male rats. J Reproduction Development, 52: 219–228.
• 22. Rezvanfar AM, Sadrkhanlou RA, Ahmadi A, Shojaei-Sadee H, Rezvanfar M, Mohammadirad A, Salehnia A, Abdollahi M (2008) Protection of cyclophosphamide-induced toxicity in reproductive tract histology, sperm characteristics, and DNA damage by an herbal source; evidence for role of free-radical toxic stress. Human Experimental Toxicol, 27: 901–910.
• 23. Selvakumar E, Prahalathan C, Sudharsan PT, Varalakshmi P (2006) Protective effect of lipoic acid on cyclophosphamide-induced testicular toxicity. Clinica Chimica Acta, 367: 114–119.
• 24. Tripathi D, Jena G (2008) Astaxanthin inhibits cytotoxic and genotoxic effects of cyclophosphamide in mice germ cells. Toxicology, 248: 96–103.
• 25. Vigueras-Villaseñor RM, Ojeda I, Gutierrez-Pérez O, Chavez-Saldaña M, Cuevas O, Maria DS, Rojas-Castañeda JC (2011) Protective effect of a-tocopherol on damage to rat testes by experimental cryptorchidism. Int J Exp Pathol, 92: 131–139.
• 26. Vogl A, Vaid K, Guttman J (2008) The Sertoli cell cytoskeleton. Advances Experimental Med Biol, 636: 186–211.
• 27. Wu JC, Gregory CW, Dephilip RM (1993) Expression of E-cadherin in immature rat and mouse testis and in rat sertoli cell cultures. Biol Reproduction, 49: 1353–1361.
• 28. Zhu LJ, Zong SD, Phillips DM, Moo-Young AJ, Bardin CW (1997) Changes in the distribution of intermediate filaments in rat sertoli cells during the seminiferous epithelium cycle and postnatal development. Anatom Rec, 248: 391–405.

Identyfikatory

DOI

10.5603/FM.2014.0050