EN
The effect of harvest time, blanchnig, freezing and long term storage of fro-zen broccoli on flavonoids content in broccoli florets was studied. Two broccoli cultivars Naxos F1 and Chronos F1 grown conventionally were used in the exper-iments. The small broccoli florets of 2 cm diameter were blanched in water of temperature 96-98 °C for 2,5 minutes, cooled in cold water and next packaged into nonperforated PE bags of 250 g capacity. Samples of broccoli were frozen at temperature -25 °C and next stored for 9 months in cold room at temperature -25 °C. Before flavonoid analysis of fresh and frozen broccoli were lyophilized and homogenized to receive a homogenous material. Analyses of flavonoids were performed for fresh broccoli, blanched and frozen material, as well as after 9 months of storage at -25 °C. Quercetin and kaempferol were found in fresh broccoli florets after harvest of both cultivars Naxos F1 and Chronos F1. Total flavonoids content in fresh broccoli ranged between 205,0 mg·kg-1 d.m. and 406,6 mg·kg-1 d.m. After blanching quercetin content in broccoli has decreased by 46-91% and kaempferol by 58-75%. After storage of frozen broccoli for 9 months there was found 8,0-17,1 mg·kg-1 d.m. of quercetin and 42,0-188,3 mg·kg-1 d.m. of kaempferol.